Optimisation of glycogen quantification in mixed microbial cultures

Bioresource Technology

Volumn 118, Issue , 2012, Pages 518-525

  Lanham, Ana B ^a   Ricardo, Ana R ^a   Coma, Marta ^b   Fradinho, Joana ^a   Carvalheira, Mónica ^a   Oehmen, Adrian ^a   Carvalho, Gilda ^a,c   Reis, Maria A M ^a  

^a DEPARTAMENTO DE QUÍMICA   (Portugal)

^b UNIVERSITY OF GIRONA   (Spain)

^c INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

Author keywords

Carbohydrate analysis; Design of experiments; Floccular and granular sludge; Glycogen quantification; Response surface modelling

Indexed keywords

ACID CONCENTRATIONS; ACID HYDROLYSIS; ACTIVATED SLUDGE; BIOMASS CONCENTRATIONS; CARBOHYDRATE ANALYSIS; GLYCOGEN QUANTIFICATION; GRANULAR SAMPLES; GRANULAR SLUDGE; MIXED MICROBIAL CULTURE; NUTRIENT REMOVAL; OPTIMAL CONDITIONS; OPTIMISATIONS; RESPONSE SURFACE MODELLING;

BIOMASS; CARBOHYDRATES; DESIGN OF EXPERIMENTS; OPTIMIZATION; SURFACE PROPERTIES;

HYDROLYSIS;

GLYCOGEN; HYDROCHLORIC ACID;

BIOMASS; CARBOHYDRATE; EXPERIMENTAL DESIGN; FLOCCULATION; GRANULAR MEDIUM; HYDROCHLORIC ACID; HYDROLYSIS; MICROBIAL ACTIVITY; OPTIMIZATION; PHOTOSYNTHESIS; QUANTITATIVE ANALYSIS; SAMPLING;

ACIDITY; ACTIVATED SLUDGE; ANALYTICAL ERROR; ARTICLE; BACTERIUM CULTURE; BIOMASS; HYDROLYSIS; MIXED CELL CULTURE; MIXED MICROBIAL CULTURE; NONHUMAN; PHOTOSYNTHESIS; PRIORITY JOURNAL; REACTION TIME;

ANALYSIS OF VARIANCE; BACTERIA; BIOMASS; CELL CULTURE TECHNIQUES; FLOCCULATION; GLUCOSE; GLYCOGEN; HYDROLYSIS; KINETICS; MODELS, BIOLOGICAL; REPRODUCIBILITY OF RESULTS;

EID: 84862295967     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2012.05.087     Document Type: Article

References (26)

1. Apostolides Z., Potgieter D. Determination of PHB in activated sludge by a gas chromatographic method. Appl. Microbiol. Biotechnol. 1981, 13:62-63.
2. Bond P.L., Erhart R., Wagner M., Keller J., Blackall L.L. Identification of some of the major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems. Appl. Environ. Microbiol. 1999, 65:4077-4084.
3. Brdjanovic D., Van Loosdrecht M.C.M., Versteeg P., Hooijmans C.M., Alaerts G.J., Heijnen J.J. Modeling COD, N and P removal in a full-scale wwtp Haarlem Waarderpolder. Water Res. 2000, 34:846-858.
4. Carvalho G., Lemos P.C., Oehmen A., Reis M.A.M. Denitrifying phosphorus removal: linking the process performance with the microbial community structure. Water Res. 2007, 41:4383-4396.
5. Cela R. Supersaturated experimental designs. New approaches to building and using it. Part I: Building optimal supersaturated designs by means of evolutionary algorithms. Chemom. Intell. Lab. Syst. 2000, 52:167-182.
6. Coats E.R., Mockos A., Loge F.J. Post-anoxic denitrification driven by PHA and glycogen within enhanced biological phosphorus removal. Bioresour. Technol. 2011, 102:1019-1027.
7. Eriksson L., Johansson E., Kettaneh-Wold N., Wikström C., Wold S. Design of Experiments: Principles and Applications 2008, Umetrics AB, Sweden. third ed.
8. Ernst A., Kirschenlohr H., Diez J., Böger P. Glycogen content and nitrogenase activity in Anabaena variabilis. Arch. Microbiol. 1984, 140:120-125.
9. Fang H., Liu Y. Intracellular polymers in aerobic sludge of sequencing batch reactors. J. Environ. Eng. 2000, 126:732.
10. Good C., Kramer H., Somogyi M. The determination of glycogen. J. Biol. Chem. 1933, 100:485.
11. Guisasola a., Vargas M., Marcelino M., Lafuente J., Casas C., Baeza J.A. On-line monitoring of the enhanced biological phosphorus removal process using respirometry and titrimetry. Biochem. Eng. J. 2007, 35:371-379.
12. Kuba T., Murnleitner E., Van Loosdrecht M., Heijnen J. A metabolic model for biological phosphorus removal by denitrifying organisms. Biochem. Eng. J. 1996, 52:685-695.
13. Lopez-Vazquez C.M., Oehmen A., Hooijmans C.M., Brdjanovic D., Gijzen H.J., Yuan Z., Van Loosdrecht M.C.M. Modeling the PAO-GAO competition: effects of carbon source, pH and temperature. Water Res. 2009, 43:450-462.
14. Lundstedt T. Experimental design and optimization. Chemom. Intell. Lab. Syst. 1998, 42:3-40.
15. Maurer M., Gujer W., Hany R., Bachmann S. Intracellular carbon flow in phosphorus accumulating organisms from activated sludge systems. Water Res. 1997, 31:907-917.
16. Montgomery D.C. Design and Analysis of Experiments 2000, Wiley. fifth ed.
17. Oehmen A., Carvalho G., Lopez-Vazquez C.M., Van Loosdrecht M.C.M., Reis M.a.M. Incorporating microbial ecology into the metabolic modelling of polyphosphate accumulating organisms and glycogen accumulating organisms. Water Res. 2010, 44:4992-5004.
18. Oehmen A., Zeng R.J., Saunders A.M., Blackall L.L., Keller J., Yuan Z. Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source. Microbiology 2006, 152:2767-2778.
19. Palmstierna H. Glycogen-like polyglucose in Escherichia coli B during the first hours of growth. Acta Chem. Scand. 1956, 10:567-577.
20. Parrou J.L., François J. A simplified procedure for a rapid and reliable assay of both glycogen and trehalose in whole yeast cells. Anal. Biochem. 1997, 248:186-188.
21. Preiss J. Bacterial glycogen synthesis and its regulation. Annu. Rev. Microbiol. 1984, 38:419-458.
22. Serafim L.S., Lemos P.C., Levantesi C., Tandoi V., Santos H., Reis M.A.M. Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms. J. Microbiol. Methods 2002, 51:1-18.
23. Slimestad R., Vågen I.M. Thermal stability of glucose and other sugar aldoses in normal phase high performance liquid chromatography. J. Chromatogr. A 2006, 1118:281-284.
24. Smolders G.J.F., Van der Meij J., Van Loosdrecht M.C.M., Heijnen J.J. Model of the anaerobic metabolism of the biological phosphorus removal process: stoichiometry and pH influence. Biochem. Eng. J. 1994, 43:461-470.
25. Van Loosdrecht M., Pot M., Heijnen J. Importance of bacterial storage polymers in bioprocesses. Water Sci. Technol. 1997, 35:41-47.
26. Zeng R.J., Saunders A.M., Yuan Z., Blackall L.L. Identification and comparison of aerobic and denitrifying polyphosphate-accumulating organisms. Biotechnol. Bioeng. 2003, 83:140-148.