Evaluation of sample preparation methods for MALDI-TOF MS identification of highly dangerous bacteria

Letters in Applied Microbiology

Volumn 55, Issue 1, 2012, Pages 40-46

  Drevinek, M ^a   Dresler, J ^b,c   Klimentova, J ^b   Pisa, L ^c   Hubalek, M ^b  

^a Statni ustav jaderne chemicke a biologicke ochrany vvi Pribram Kamenna   (Czech Republic)

^b UNIVERSITY OF DEFENCE   (Czech Republic)

^c Central Military Institute of Health   (Czech Republic)

Author keywords

High risk bacterial pathogens; MALDI TOF; Sample preparation

Indexed keywords

BACTERIOLOGY; ETHANOL; INDUCTIVELY COUPLED PLASMA; MASS SPECTROMETRY; PROTEINS; QUALITY CONTROL;

BACTERIAL PATHOGENS; BACTERIAL SPECIES; HIGH-RISK BACTERIAL PATHOGEN; MALDI TOF MS; MALDI-TOF; PATHOGENIC BACTERIUM; SAMPLE PREPARATION; SAMPLE PREPARATION METHODS; SPECTRAL QUALITY; WORK-FLOWS;

EXTRACTION;

ACETONITRILE; ALCOHOL; FORMIC ACID;

BACTERIUM; BIOCHEMICAL COMPOSITION; ETHANOL; IDENTIFICATION METHOD; PATHOGEN; PROTEIN; RISK ASSESSMENT; SAMPLE PREPARATION; STORAGE; TOXIC ORGANISM; VIRULENCE;

ARTICLE; BACILLUS ANTHRACIS; BACILLUS CEREUS; BACTERIUM IDENTIFICATION; BRUCELLA MELITENSIS; BURKHOLDERIA MALLEI; CLINICAL EVALUATION; CLOSTRIDIUM BOTULINUM; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CORYNEBACTERIUM DIPHTHERIAE; CORYNEBACTERIUM ULCERANS; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; FRANCISELLA TULARENSIS; LEGIONELLA PNEUMOPHILA; LISTERIA MONOCYTOGENES; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; SHIGELLA DYSENTERIAE; SPECTROPHOTOMETRY; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES; VIBRIO CHOLERAE; WORKFLOW; YERSINIA PESTIS;

BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); BRUCELLA MELITENSIS; BURKHOLDERIA MALLEI; CLOSTRIDIUM BOTULINUM; FRANCISELLA TULARENSIS; LEGIONELLA PNEUMOPHILA; NEGIBACTERIA; POSIBACTERIA; SHIGELLA DYSENTERIAE; VIBRIO CHOLERAE; YERSINIA PESTIS;

EID: 84862266130     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2012.03255.x     Document Type: Article

References (11)

1. Demirev, P.A., Ho, Y.P., Ryzhov, V. and Fenselau, C. (1999) Microorganism identification by mass spectrometry and protein database searches. Anal Chem 71, 2732-2738.
2. Fox, A. (2006) Mass spectrometry for species or strain identification after culture or without culture: past, present, and future. J Clin Microbiol 44, 2677-2680.
3. Hernychova, L., Toman, R., Ciampor, F., Hubalek, M., Vackova, J., Macela, A. and Skultety, L. (2008) Detection and identification of Coxiella burnetii based on the mass spectrometric analyses of the extracted proteins. Anal Chem 80, 7097-7104.
4. Horneffer, V., Haverkamp, J., Janssen, H.G., ter Steeg, P.F. and Notz, R. (2004) MALDI-TOF-MS analysis of bacterial spores: wet heat-treatment as a new releasing technique for biomarkers and the influence of different experimental parameters and microbiological handling. J Am Soc Mass Spectrom 15, 1444-1454.
5. Lasch, P., Nattermann, H., Erhard, M., Stammler, M., Grunow, R., Bannert, N., Appel, B. and Naumann, D. (2008) MALDI-TOF mass spectrometry compatible inactivation method for highly pathogenic microbial cells and spores. Anal Chem 80, 2026-2034.
6. Liu, H., Du, Z., Wang, J. and Yang, R. (2007) Universal sample preparation method for characterization of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl Environ Microbiol 73, 1899-1907.
7. Marklein, G., Josten, M., Klanke, U., Muller, E., Horre, R., Maier, T., Wenzel, T., Kostrzewa, M. et al. (2009) Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates. J Clin Microbiol 47, 2912-2917.
8. Mellmann, A., Bimet, F., Bizet, C., Borovskaya, A.D., Drake, R.R., Eigner, U., Fahr, A.M., He, Y. et al. (2009) High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria. J Clin Microbiol 47, 3732-3734.
9. Setlow, P. (2006) Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals. J Appl Microbiol 101, 514-525.
10. Vargha, M., Takats, Z., Konopka, A. and Nakatsu, C.H. (2006) Optimization of MALDI-TOF MS for strain level differentiation of Arthrobacter isolates. J Microbiol Methods 66, 399-409.
11. Wahl, K.L., Wunschel, S.C., Jarman, K.H., Valentine, N.B., Petersen, C. E., Kingsley, M.T., Zartolas, K.A. and Saenz, A.J. (2002) Analysis of microbial mixtures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem 74, 6191-6199.