A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children

Malaria Journal

Volumn 11, Issue , 2012, Pages

  Lamikanra, Abigail A ^a,b   Dobão, Carlota ^c,d   Jiménez, Alfons ^c   Nhabomba, Augusto ^d   Tsang, Hoi P ^a,b   Guinovart, Caterina ^c,d   Manaca, Maria N ^d   Quinto, Llorenç ^c   Aguilar, Ruth ^c,d   Cisterá, Pau ^c   Alonso, Pedro L ^c,d   Roberts, David J ^a,b   Mayor, Alfredo ^c,d  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^c CENTRE FOR RESEARCH IN ENVIRONMENTAL EPIDEMIOLOGY CREAL   (Spain)

^d Ministérioda Saúde   (Mozambique)

Author keywords

Anaemia; Malaria; Plasma; qPCR

Indexed keywords

RNA 18S; PROTOZOAL RNA;

ANEMIA; ARTICLE; BINOMIAL DISTRIBUTION; BLOOD; CHILD; CORRELATION ANALYSIS; CORRELATION COEFFICIENT; FISHER EXACT TEST; HUMAN; INCIDENCE; INFANT; KAPPA STATISTICS; MALARIA FALCIPARUM; MCNEMAR TEST; MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE; PARASITEMIA; PLASMA; PLASMODIUM FALCIPARUM; PRESCHOOL CHILD; PREVALENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SMEAR; ANIMAL; COMPARATIVE STUDY; EVALUATION; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MOLECULAR DIAGNOSIS; NEWBORN; PARASITOLOGY; PREGNANCY; SENSITIVITY AND SPECIFICITY;

ANIMALS; BLOOD; CHILD, PRESCHOOL; FEMALE; HUMANS; INFANT; INFANT, NEWBORN; MALARIA, FALCIPARUM; MALE; MICROSCOPY; MOLECULAR DIAGNOSTIC TECHNIQUES; PARASITEMIA; PARASITOLOGY; PLASMODIUM FALCIPARUM; PREGNANCY; REAL-TIME POLYMERASE CHAIN REACTION; RNA, PROTOZOAN; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY;

EID: 84862216520     PISSN: None     EISSN: 14752875     Source Type: Journal    
DOI: 10.1186/1475-2875-11-201     Document Type: Article

References (16)

1. WHO, World Malaria Report 2011 Geneva: World Health Organization 2011 http://www.who.int/malaria/world-malaria-report-2011/9789241564403-eng.pdf
2. A research agenda for malaria eradication: diagnoses and diagnostics. malERA Consultative Group on Diagnoses and Diagnostics, PLoS Med 2011 8 1000396 21311583
3. Detection of Plasmodium falciparum DNA in plasma. Gal S, Fidler C, Turner S, Lo YM, Roberts DJ, Wainscoat JS, Ann N Y Acad Sci 2001 945 234 238 11708485 (Pubitemid 33022459)
4. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. Rougemont M, Van Saanen M, Sahli R, Hinrikson HP, Bille J, Jaton K, J Clin Microbiol 2004 42 5636 5643 10.1128/JCM.42.12.5636-5643.2004 15583293 (Pubitemid 39664347)
5. Quantitative real-time polymerase chain reaction for malaria diagnosis and its use in malaria vaccine clinical trials. Andrews L, Andersen RF, Webster D, Dunachie S, Walther RM, Bejon P, Hunt-Cooke A, Bergson G, Sanderson F, Hill AV, Gilbert SC, Am J Trop Med Hyg 2005 73 191 198 16014857 (Pubitemid 41278771)
6. Thick blood film examination for Plasmodium falciparum malaria has reduced sensitivity and underestimates parasite density. Bejon P, Andrews L, Hunt-Cooke A, Sanderson F, Gilbert SC, Hill AV, Malar J 2006 5 104 10.1186/1475-2875-5-104 17092336
7. Efficacy of the RTS, S/AS02A vaccine against Plasmodium falciparum infection and disease in young African children: randomised controlled trial. Alonso PL, Sacarlal J, Aponte JJ, Leach A, Macete E, Milman J, Mandomando I, Spiessens B, Guinovart C, Espasa M, Bassat Q, Aide P, Ofori-Anyinam O, Navia MM, Corachan S, Ceuppens M, Dubois MC, Demoité MA, Dubovsky F, Menéndez C, Tornirporth N, Ballou WR, Thompson R, Cohen J, Lancet 2004 364 1411 1420 10.1016/S0140-6736(04)17223-1 15488216 (Pubitemid 39370716)
8. The role of age and exposure to Plasmodium falciparum in the rate of acquisition of naturally acquired immunity: a randomized controlled trial. Guinovart C, Dobão C, Bassat Q, Nhabomba A, Quint L, Nélia Manaca M, Aguilar R, Rodríguez M, Barbosa A, Aponte J, Mayor A, Renom M, Moraleda C, Roberts DJ, Schwarzer E, Le Souf PN, Schofield L, Chitnis CE, Doolan DL, Alonso PL, PloS ONE 2012 7 32362 10.1371/journal.pone.0032362 22412865
9. Sub-microscopic infections and long-term recrudescence of Plasmodium falciparum in Mozambican pregnant women. Mayor A, Serra-Casas E, Bardaji A, Sanz S, Puyol L, Cistero P, Sigauque B, Mandomando I, Aponte JJ, Alonso PL, Menéndez C, Malar J 2009 8 9 10.1186/1475-2875-8-9 19134201
10. Prevalence and density related concordance of three diagnostic tests for malaria in hypoendemic Tanzania. Schachterle S, Mtove G, Levens J, Clemens EG, Shi L, Raj A, Munoz B, Reller ME, West S, Dumler JS, Sullivan D, PRET Project Team, J Clin Microbiol 2011 49 3885 3891 10.1128/JCM.01157-11 21880972
11. Malaria prevalence defined by microscopy, antigen detection, DNA amplification and total nucleic acid amplification in a malaria-endemic region during the peak malaria transmission season. Waitumbi JN, Gerlach J, Afonina I, Anyona SB, Koros JN, Siangla J, Ankoudinova I, Singhal M, Watts K, Polhemus ME, Vermeulen NM, Mahoney W, Steele M, Domingo GJ, Trop Med Int Health 2011 16 786 793 10.1111/j.1365-3156.2011.02773.x 21447064
12. Reduction in the proportion of fevers associated with Plasmodium falciparum parasitaemia in Africa: a systematic review. D'Acremont V, Lengeler C, Genton B, Malar J 2010 9 240 10.1186/1475-2875-9-240 20727214
13. Coma associated with microscopy-diagnosed Plasmodium vivax: a prospective study in Papua. Lampah DA, Yeo TW, Hardianto SO, Tjitra E, Kenangalem E, Sugiarto P, Price RN, Anstey NM, Indonesia. PLoS Negl Trop Dis 2011 5 1032 10.1371/journal.pntd.0001032
14. Plasmodium falciparum infections are associated with agglutinating antibodies to parasite-infected erythrocyte surface antigens among healthy Kenyan children. Bull PC, Lowe BS, Kaleli N, Njuga M, Kortok M, Ross A, Ndungu F, Snow RW, Marsh K, J Infect Dis 2002 185 1688 1691 10.1086/340420 12023781 (Pubitemid 34587537)
15. Malaria diagnosis by a polymerase chain reaction-based assay using a pooling strategy. Bharti AR, Letendre SL, Patra KP, Vinetz JM, Smith DM, Am J Trop Med Hyg 2009 81 754 757 10.4269/ajtmh.2009.09-0274 19861605
16. Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T, J Clin Microbiol 2007 45 2521 2528 10.1128/JCM.02117-06 17567794 (Pubitemid 47295483)