Preparation of a polyclonal antibody based heterologous indirect competitive ELISA for detecting ractopamine residue

Journal of Food, Agriculture and Environment

Volumn 10, Issue 2, 2012, Pages 1349-1352

  Li, Chao Ying ^a,b   Jiang, Jin Qing ^b  

^a XINXIANG MEDICAL UNIVERSITY   (China)

^b HENAN INSTITUTE OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Artificial antigen; Heterology; Indirect competitive ELISA; Polyclonal antibody; Ractopamine

Indexed keywords

ACID ANHYDRIDE; ANTIGEN; DOBUTAMINE; DRUG RESIDUE; ETHER DERIVATIVE; POLYCLONAL ANTIBODY; RACTOPAMINE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN PURIFICATION; ARTICLE; CATTLE; CROSS REACTION; DRUG DETERMINATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; IC 50; INDIRECT COMPETITIVE ENZYME LINKED IMMUNOSORBENT ASSAY; LIMIT OF DETECTION; MUSCLE; NONHUMAN; RABBIT; SWINE; TITRIMETRY;

EID: 84862210116     PISSN: 14590255     EISSN: 14590263     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Zuo, P., Zhang, Y., Liu, J. and Ye, B. C. 2010. Determination of beta-adrenergic agonists by hapten microarray. Talanta 82:61-66.
2. Dickson, L. C., MacNeil, J. D., Lee, S. and Fesser, A. C. 2005. Determination of beta-agonist residues in bovine urine using liquid chromatography-tandem mass spectrometry. J. A. O. A. C. Int. 88:46-56.
3. Mitchell, G. A. and Dunnavan, G. 1998. Illegal use of beta-adrenergic agonists in the United States. J. Anim. Sci. 76:208-211.
4. Liu, X., He, X., Moore, C., Wang, G. and Coulter, C. 2009. Highly sensitive and specific liquid chromatography-tandem mass spectrometry method for testing ractopamine in cow and sheep urine. J. Anal. Toxicol. 33:289-193.
5. Wang, J. P., Li, X. W., Zhang, W. and Shen, J. Z. 2006. Development of immunoaffinity sample-purification for GC-MS analysis of ractopamine in swine tissue. Chromatographic 64:613-617.
6. Bocca, B., Fiori, M., Cartoni, C. and Brambilla, G. 2003. Simultaneous determination of zilpaterol and other beta agonists in calf eye by gas chromatography/tandem mass spectrometry. J. A. O. A. C. Int. 86:8-14.
7. Shelver, W. L., Smith, D. J. and Berry, E. S. 2000. Production and characterization of a monoclonal antibody against the beta-adrenergic agonist ractopamine. J. Agric. Food Chem. 48:4020-4026.
8. Shelver, W. L. and Smith D. J. 2002. Application of a monoclonal antibody-based enzyme-linked immunosorbent assay for the determination of ractopamine in incurred samples from food animals. J. Agric. Food Chem. 50:2742-2747.
9. Li, X., Zhang, G., Deng, R., Yang, Y., Liu, Q., Xiao, Z., Yang, J., Xing, G., Zhao, D. and Cai, S. 2010. Development of rapid immunoassays for the detection of ractopamine in swine urine. Food Addit. Contam. 27:1096-1103.
10. Jiang, J. Q., Zhang, H. T., Li, G. L., Wang, Z. L., Wang, J. H. and Zhao, H. Z. 2011. Preparation of anti-nortestosterone antibodies and development of an indirect heterologous competitive enzyme-linked immunosorbent assay to detect nortestosterone residues in animal urine. Anal. Lett. 44:2373-2393.
11. Jiang, J., Wang, Z., Zhang, H., Zhang, X., Liu, X. and Wang, S. 2011. Monoclonal antibody-based ELISA and colloidal gold immunoassay for detecting NT residue in animal tissues. J. Agric. Food Chem. 59:9763-9769.
12. Zhang, L., Jiang, J. Q., Li, G. L., Wang, Z. L., Yang, X. F., Ma, J. Y., Zhang, H. T., Huang, H. G. and Liu, X. Y. 2011. Development and optimization of a monoclonal antibody-based indirect competitive ELISA for detecting fluoroquinolone residue in milk. J. Food Agric. Environ. 9(2):113-120.