Differentiation and visualization of diverse cellular phenotypic responses in primary high-content screening

Journal of Biomolecular Screening

Volumn 17, Issue 6, 2012, Pages 843-849

  Kümmel, Anne ^a   Selzer, Paul ^b   Siebert, Daniela ^b   Schmidt, Isabel ^b   Reinhardt, Jürgen ^b   Götte, Marjo ^b   Ibig Rehm, Yvonne ^b   Parker, Christian N ^c   Gabriel, Daniela ^b,c  

^a Modeling and Simulation   (Switzerland)

^b Lead Finding Platform   (Switzerland)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

cell based assays; chemoinformatics; high content screening; imaging technologies; multiplex assays and technology

Indexed keywords

ARTICLE; CELL DIFFERENTIATION; CLUSTER ANALYSIS; HIGH THROUGHPUT SCREENING; PHENOTYPE; PRIORITY JOURNAL;

ANIMALS; CHO CELLS; CLUSTER ANALYSIS; CRICETINAE; CRICETULUS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DRUG EVALUATION, PRECLINICAL; HIGH-THROUGHPUT SCREENING ASSAYS; IMAGE PROCESSING, COMPUTER-ASSISTED; LASER SCANNING CYTOMETRY; MULTIVARIATE ANALYSIS; PHENOTYPE; PRINCIPAL COMPONENT ANALYSIS;

EID: 84862188419     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112439324     Document Type: Article

References (16)

1. Loo L. H., Wu L. F., Altschuler S. J.. Image-Based Multivariate Profiling of Drug Responses from Single Cells. Nat. Methods. 2007 ; 4: 445-453
2. Perlman Z. E., Slack M. D., Feng Y., Mitchison T. J., Wu L. F., Altschuler S. J.. Multidimensional Drug Profiling by Automated Microscopy. Science. 2004 ; 306: 1194-1198
3. Young D. W., Bender A., Hoyt J., McWhinnie E., Chirn G. W., Tao C. Y., Tallarico J. A., Labow M., Jenkins J. L., Mitchison T. J., et al. Integrating High-Content Screening and Ligand-Target Prediction to Identify Mechanism of Action. Nat. Chem. Biol. 2008 ; 4: 59-68
4. Denner P., Schmalowsky J., Prechtl S.. High-Content Analysis in Preclinical Drug Discovery. Comb. Chem. High Throughput Screen. 2008 ; 11: 216-230
5. Nichols A.. High Content Screening as a Screening Tool in Drug Discovery. Methods Mol. Biol. 2007 ; 356: 379-387
6. Wolff M., Kredel S., Haasen D., Wiedenmann J., Nienhaus G. U., Kistler B., Oswald F., Heilker R.. High Content Screening of CXCR2-Dependent Signalling Pathways. Comb. Chem. High Throughput Screen. 2010 ; 13: 3-15
7. Duerr O., Duval F., Nichols A., Lang P., Brodte A., Heyse S., Besson D.. Robust Hit Identification by Quality Assurance and Multivariate Data Analysis of a High-Content, Cell-Based Assay. J. Biomol. Screen. 2007 ; 12: 1042-1049
8. Thalamuthu A., Mukhopadhyay I., Zheng X., Tseng G. C.. Evaluation and Comparison of Gene Clustering Methods in Microarray Analysis. Bioinformatics. 2006 ; 22: 2405-2412
9. Huang S.. Classification of Cell Subpopulations Using Multiple Cellular Parameters from High-Content Imaging Studies. J. Biomol. Screen. 2008 ; 13: 941-952
10. Caie P. D., Walls R. E., Ingleston-Orme A., Daya S., Houslay T., Eagle R., Roberts M. E., Carragher N. O.. High-Content Phenotypic Profiling of Drug Response Signatures across Distinct Cancer Cells. Mol. Cancer Ther. 9: 1913-1926
11. Ameen N., Silvis M., Bradbury N. A.. Endocytic Trafficking of CFTR in Health and Disease. J. Cyst. Fibros. 2007 ; 6: 1-14
12. Cholon D. M., O'Neal W. K., Randell S. H., Riordan J. R., Gentzsch M.. Modulation of Endocytic Trafficking and Apical Stability of CFTR in Primary Human Airway Epithelial Cultures. Am. J. Physiol. Lung Cell Mol. Physiol. 2010 ; 298: L304 - L314
13. Gubler H.. Assay Data Quality Assessment. Methods Mol. Biol. 2009 ; 552: 79-95
14. Galietta L. V. J., Jayaraman S., Verkman A. S.. Cell-Based Assay for High-Throughput Quantitative Screening of CFTR Chloride Transport Agonists. Am. J. Physiol. Cell Physiol. 2001 ; 281: C1734 - C1742
15. Fliri A. F., Loging W. T., Thadeio P. F., Volkmann R. A.. Biological Spectra Analysis: Linking Biological Activity Profiles to Molecular Structure. Proc. Natl. Acad. Sci. U. S. A. 2005 ; 102: 261-266
16. Boehm H. J., Flohr A., Stahl M.. Scaffold Hopping. Drug Discov. Today. 2004 ; 1: 217-224