Rapid and simplified purification of recombinant adeno-associated virus

Journal of Virological Methods

Volumn 183, Issue 2, 2012, Pages 139-146

  Guo, Ping ^a   El Gohary, Yousef ^a   Prasadan, Krishna ^a   Shiota, Chiyo ^a   Xiao, Xiangwei ^a   Wiersch, John ^a   Paredes, Jose ^a   Tulachan, Sidhartha ^b   Gittes, George K ^a  

^a CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

^b ST ELIZABETH HEALTH CENTER   (United States)

Author keywords

AAV8; Aqueous two phase partition system (ATPs)

Indexed keywords

CESIUM CHLORIDE; IODIXANOL; MACROGOL 8000; POLYETHYLENEIMINE; SHORT HAIRPIN RNA; VIRUS PROTEIN;

ADENO ASSOCIATED VIRUS; ANIMAL EXPERIMENT; ANIMAL TISSUE; AQUEOUS TWO PHASE PARTITIONING; ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DNA TRANSFECTION; DOWNSTREAM PROCESSING; EMBRYO; IN VITRO GENE TRANSFER; MOUSE; NONHUMAN; PHASE PARTITIONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SEROTYPE; SILVER STAINING; ULTRAHIGH SPEED DENSITY GRADIENT CENTRIFUGATION; VIRAL GENE DELIVERY SYSTEM; VIRUS CULTURE; VIRUS GENE; VIRUS PARTICLE; VIRUS PURIFICATION; VIRUS RECOMBINANT; VIRUS TITRATION; WESTERN BLOTTING;

AMMONIUM SULFATE; ANIMALS; BUFFERS; CARBONATES; CHEMICAL PRECIPITATION; DEPENDOVIRUS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; LIQUID-LIQUID EXTRACTION; LIVER; MICE; ORGANISMS, GENETICALLY MODIFIED; POLYETHYLENE GLYCOLS; SALINE SOLUTION, HYPERTONIC; TRANSDUCTION, GENETIC;

ADENO-ASSOCIATED VIRUS; MUS;

EID: 84862153648     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.04.004     Document Type: Article

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