Expression and purification of human TAT-p53 fusion protein in Pichia pastoris and its influence on HepG2 cell apoptosis

Biotechnology Letters

Volumn 34, Issue 7, 2012, Pages 1217-1223

  Yan, Haowei ^a   Liu, Nan ^a   Zhao, Zhenghong ^b   Zhang, Xinmin ^a   Xu, Hao ^a   Shao, Bing ^a   Yan, Weiqun ^a  

^a JILIN UNIVERSITY   (China)

^b Changchun Children's Hospital   (China)

Author keywords

Apoptosis; HepG2; p53; Pichia pastoris; Tat p53

Indexed keywords

CANCER CELL LINES; CANCER THERAPEUTICS; CELL-CYCLE ARREST; EXPRESSION SYSTEM; FUSION PROTEINS; HEPG2; HEPG2 CELLS; HIV TAT; P53; PICHIA PASTORIS; PROTEIN YIELD; TAT-P53; THERAPEUTIC STRATEGY; TUMOR CELLS;

CELL CULTURE; CELL MEMBRANES; DISEASES; PROTEINS; YEAST;

CELL DEATH;

HYBRID PROTEIN; PROTEIN P53; TRANSACTIVATOR PROTEIN;

APOPTOSIS; ARTICLE; BIOSYNTHESIS; CELL STRAIN HEPG2; DRUG EFFECT; GENE EXPRESSION; GENETIC TRANSDUCTION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LIVER CELL; PHYSIOLOGY; PICHIA;

APOPTOSIS; GENE EXPRESSION; HEP G2 CELLS; HEPATOCYTES; HUMANS; PICHIA; RECOMBINANT FUSION PROTEINS; TAT GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; TRANSDUCTION, GENETIC; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84862142346     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-0905-8     Document Type: Article

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