Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry

Journal of Clinical Microbiology

Volumn 50, Issue 6, 2012, Pages 2137-2139

  Hegde, Narasimha V ^a   Jayarao, Bhushan M ^a   DebRoy, Chitrita ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYCLONAL ANTIBODY;

ANTIGEN ANTIBODY REACTION; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; BEEF; CITROBACTER FREUNDII; CONTROLLED STUDY; ENTEROBACTER CLOACAE; FLOW CYTOMETRY; FOOD SAFETY; HAFNIA ALVEI; KLEBSIELLA PNEUMONIAE; NONHUMAN; PRIORITY JOURNAL; PROTEUS VULGARIS; SALMONELLA ENTERITIDIS; SALMONELLA TYPHI; SERRATIA MARCESCENS; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SHIGELLA BOYDII; SHIGELLA FLEXNERI;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CATTLE; FLOW CYTOMETRY; MEAT; SENSITIVITY AND SPECIFICITY; SHIGA-TOXIGENIC ESCHERICHIA COLI; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84862080061     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00137-12     Document Type: Article

References (10)

1. DebRoy C, Roberts E, Valadez AM, Dudley EG, Cutter CN. 2011. Detection of Shiga toxin-producing E. coli O26, O45, O103, O111, O113, O121, O145 and O157 serogroups by multiplex PCR of the wzx gene of the O-antigen cluster. Foodborne Pathog. Dis. 8:651-652.
2. DebRoy C, Roberts E, Fratamico PM. 2011. Detection of O antigens in Escherichia coli. Anim. Health Res. Rev. 12:169-185.
3. Ferrari BC, Oregaard G, Sørensen SJ. 2004. Recovery of GFP-labeled bacteria for culturing and molecular analysis after cell sorting using a benchtop flow cytometer. Microb. Ecol. 48:239-245. (Pubitemid 40383326)
4. Fratamico PM, et al. 2011. Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef. Foodborne Pathog. Dis. 8:601-607.
5. Nebe-von-Caron G, Stephens PJ, Hewitt CJ, Powell JR, Badley RA. 2000. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods 42:97-114.
6. Possé B, De Zutter L, Heyndrickx M, Herman L. 2008. Quantitative isolation efficiency of O26, O103, O111, O145 and O157 STEC serotypes from artificially contaminated food and cattle faeces samples using a new isolation protocol. J. Appl. Microbiol. 105:227-235. (Pubitemid 351793563)
7. Shapiro HM. 2000. Microbial analysis at the single-cell level: tasks and techniques. J. Microbiol. Methods 42:3-16.
8. USDA Food Safety and Inspection Service. 2011. Risk profile for pathogenic non-O157 Shiga toxin-producing Escherichia coli (non-O157 STEC). USDA Food Safety and Inspection Service, Washington, DC. http://www.fsis.usda.gov/ pdf/non-o157-stec-risk-profile.pdf.
9. Valadez AM, DebRoy C, Dudley EG, Cutter CN. 2011. Multiplex PCR detection of Shiga toxin-producing Escherichia coli strains belonging to serogroups O157, O103, O91, O113, O145, O111, and O26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef. J. Food. Prot. 74:228-239.
10. Yang L, et al. 2010. Rapid, absolute, and simultaneous quantification of specific pathogenic strain and total bacterial cells using an ultrasensitive dual-color flow cytometer. Anal. Chem. 82:1109-1116.