-
2
-
-
77649305316
-
BglBricks: A flexible standard for biological part assembly
-
Anderson J, Dueber J, Leguia M, Wu G, Goler J, et al. (2010) BglBricks: A flexible standard for biological part assembly. Journal of biological engineering 4: 1-12.
-
(2010)
Journal of Biological Engineering
, vol.4
, pp. 1-12
-
-
Anderson, J.1
Dueber, J.2
Leguia, M.3
Wu, G.4
Goler, J.5
-
3
-
-
0033676098
-
DNA Cloning Using In Vitro Site-Specific Recombination
-
Hartley J, (2000) DNA Cloning Using In Vitro Site-Specific Recombination. Genome Research 10: 1788-1795.
-
(2000)
Genome Research
, vol.10
, pp. 1788-1795
-
-
Hartley, J.1
-
4
-
-
33847608289
-
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
-
Li M, Elledge S, (2007) Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nature Methods 4: 251-256.
-
(2007)
Nature Methods
, vol.4
, pp. 251-256
-
-
Li, M.1
Elledge, S.2
-
5
-
-
0025085655
-
Ligation-independent cloning of PCR products (LIC-PCR)
-
Aslanidis C, de Jong PJ, (1990) Ligation-independent cloning of PCR products (LIC-PCR). Nucleic acids research 18: 6069-6074.
-
(1990)
Nucleic Acids Research
, vol.18
, pp. 6069-6074
-
-
Aslanidis, C.1
de Jong, P.J.2
-
6
-
-
67349270900
-
Enzymatic assembly of DNA molecules up to several hundred kilobases
-
Gibson D, Young L, Chuang R, Venter J, Hutchison C, et al. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods 6: 343-345.
-
(2009)
Nature Methods
, vol.6
, pp. 343-345
-
-
Gibson, D.1
Young, L.2
Chuang, R.3
Venter, J.4
Hutchison, C.5
-
7
-
-
68149136937
-
Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
-
Quan J, Tian J, (2009) Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways. PLoS ONE 4: e6441.
-
(2009)
PLoS ONE
, vol.4
-
-
Quan, J.1
Tian, J.2
-
8
-
-
84934438590
-
The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis
-
Klock H, Lesley S, (2009) The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis. Methods Mol Biol 498: 91-103.
-
(2009)
Methods Mol Biol
, vol.498
, pp. 91-103
-
-
Klock, H.1
Lesley, S.2
-
9
-
-
78049323797
-
Chemical synthesis of the mouse mitochondrial genome
-
Gibson D, Smith H, Hutchison C, Venter J, Merryman C, (2010) Chemical synthesis of the mouse mitochondrial genome. Nature Methods 7: 901-903.
-
(2010)
Nature Methods
, vol.7
, pp. 901-903
-
-
Gibson, D.1
Smith, H.2
Hutchison, C.3
Venter, J.4
Merryman, C.5
-
10
-
-
0024982030
-
Magnetic separation of DNA
-
Uhlen M, (1989) Magnetic separation of DNA. Nature 340: 733-734.
-
(1989)
Nature
, vol.340
, pp. 733-734
-
-
Uhlen, M.1
-
11
-
-
0032540905
-
A Sequencing Method Based on Real-Time Pyrophosphate
-
doi:10.1126/science.281.5375.363
-
Ronaghi M, Uhlen M, Nyren P, (1998) A Sequencing Method Based on Real-Time Pyrophosphate. Science 281: 363-365 doi:10.1126/science.281.5375.363.
-
(1998)
Science
, vol.281
, pp. 363-365
-
-
Ronaghi, M.1
Uhlen, M.2
Nyren, P.3
-
12
-
-
27644541480
-
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
-
doi:10.1093/nar/gni143
-
Kojima T, Takei Y, Ohtsuka M, Kawarasaki Y, Yamane T, et al. (2005) PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets. Nucleic acids research 33: e150 doi:10.1093/nar/gni143.
-
(2005)
Nucleic Acids Research
, vol.33
-
-
Kojima, T.1
Takei, Y.2
Ohtsuka, M.3
Kawarasaki, Y.4
Yamane, T.5
-
13
-
-
56249117019
-
Microbeads display of proteins using emulsion PCR and cell-free protein synthesis
-
Gan R, Yamanaka Y, Kojima T, Nakano H, (2008) Microbeads display of proteins using emulsion PCR and cell-free protein synthesis. Biotechnology Progress 24: 1107-1114.
-
(2008)
Biotechnology Progress
, vol.24
, pp. 1107-1114
-
-
Gan, R.1
Yamanaka, Y.2
Kojima, T.3
Nakano, H.4
-
14
-
-
79955773387
-
Parallel on-chip gene synthesis and application to optimization of protein expression
-
doi:10.1038/nbt.1847
-
Quan J, Saaem I, Tang N, Ma S, Negre N, et al. (2011) Parallel on-chip gene synthesis and application to optimization of protein expression. Nature Biotechnology 29: 449-452 doi:10.1038/nbt.1847.
-
(2011)
Nature Biotechnology
, vol.29
, pp. 449-452
-
-
Quan, J.1
Saaem, I.2
Tang, N.3
Ma, S.4
Negre, N.5
-
15
-
-
33751007558
-
A DNA biochip for on-the-spot multiplexed pathogen identification
-
doi:10.1093/nar/gkl702
-
Yeung S-W, Lee TM-H, Cai H, Hsing I-M, (2006) A DNA biochip for on-the-spot multiplexed pathogen identification. Nucleic acids research 34: e118 doi:10.1093/nar/gkl702.
-
(2006)
Nucleic Acids Research
, vol.34
-
-
Yeung, S.-W.1
Lee, T.M.-H.2
Cai, H.3
Hsing, I.-M.4
-
16
-
-
11144354637
-
Expression profiling using a hexamer-based universal microarray
-
doi:10.1038/nbt948
-
Roth ME, Feng L, McConnell KJ, Schaffer PJ, Guerra CE, et al. (2004) Expression profiling using a hexamer-based universal microarray. Nature biotechnology 22: 418-426 doi:10.1038/nbt948.
-
(2004)
Nature Biotechnology
, vol.22
, pp. 418-426
-
-
Roth, M.E.1
Feng, L.2
McConnell, K.J.3
Schaffer, P.J.4
Guerra, C.E.5
-
17
-
-
0023888944
-
Solid-Phase DNA sequencing using the biotin-avidin system
-
Stahl S, Hultman T, Olsson A, Moks T, Uhlen M, (1988) Solid-Phase DNA sequencing using the biotin-avidin system. Nucleic Acids Research 16: 3025-3038.
-
(1988)
Nucleic Acids Research
, vol.16
, pp. 3025-3038
-
-
Stahl, S.1
Hultman, T.2
Olsson, A.3
Moks, T.4
Uhlen, M.5
-
18
-
-
14944364973
-
The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures
-
Holmberg A, Blomstergren A, Nord O, Lukacs M, Lundeberg J, et al. (2005) The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures. Electrophoresis 26: 501-510.
-
(2005)
Electrophoresis
, vol.26
, pp. 501-510
-
-
Holmberg, A.1
Blomstergren, A.2
Nord, O.3
Lukacs, M.4
Lundeberg, J.5
-
19
-
-
0030024985
-
Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules
-
Smith S, Cui Y, Bustamante C, (1996) Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules. Science 271: 795.
-
(1996)
Science
, vol.271
, pp. 795
-
-
Smith, S.1
Cui, Y.2
Bustamante, C.3
-
20
-
-
8344256552
-
Recombinant protein folding and misfolding in Escherichia coli
-
doi:10.1038/nbt1029
-
Baneyx F, Mujacic M, (2004) Recombinant protein folding and misfolding in Escherichia coli. Nature biotechnology 22: 1399-1408 doi:10.1038/nbt1029.
-
(2004)
Nature Biotechnology
, vol.22
, pp. 1399-1408
-
-
Baneyx, F.1
Mujacic, M.2
-
21
-
-
14744285206
-
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
-
Daly R, Hearn MTW, (2005) Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. Journal Of Molecular Recognition 18: 119-138.
-
(2005)
Journal Of Molecular Recognition
, vol.18
, pp. 119-138
-
-
Daly, R.1
Hearn, M.T.W.2
-
22
-
-
33645417226
-
High-throughput protein purification using an automated set-up for high-yield affinity chromatography
-
doi:10.1016/j.pep.2005.12.010
-
Steen J, Uhlén M, Hober S, Ottosson J, (2006) High-throughput protein purification using an automated set-up for high-yield affinity chromatography. Protein expression and purification 46: 173-178 doi:10.1016/j.pep.2005.12.010.
-
(2006)
Protein Expression and Purification
, vol.46
, pp. 173-178
-
-
Steen, J.1
Uhlén, M.2
Hober, S.3
Ottosson, J.4
-
23
-
-
18744383075
-
Localized transfection on arrays of magnetic beads coated with PCR products
-
doi:10.1038/nmeth732
-
Isalan M, Santori MI, Gonzalez C, Serrano L, (2005) Localized transfection on arrays of magnetic beads coated with PCR products. Nature methods 2: 113-118 doi:10.1038/nmeth732.
-
(2005)
Nature Methods
, vol.2
, pp. 113-118
-
-
Isalan, M.1
Santori, M.I.2
Gonzalez, C.3
Serrano, L.4
-
24
-
-
61649088104
-
Control of steric hindrance on restriction enzyme reactions with surface-bound DNA nanostructures
-
doi:10.1021/nl802370g
-
Castronovo M, Radovic S, Grunwald C, Casalis L, Morgante M, et al. (2008) Control of steric hindrance on restriction enzyme reactions with surface-bound DNA nanostructures. Nano letters 8: 4140-4145 doi:10.1021/nl802370g.
-
(2008)
Nano Letters
, vol.8
, pp. 4140-4145
-
-
Castronovo, M.1
Radovic, S.2
Grunwald, C.3
Casalis, L.4
Morgante, M.5
-
25
-
-
0035865925
-
Hybridization of single-stranded DNA targets to immobilized complementary DNA probes: comparison of hairpin versus linear capture probes
-
Riccelli PV, Merante F, Leung KT, Bortolin S, Zastawny RL, et al. (2001) Hybridization of single-stranded DNA targets to immobilized complementary DNA probes: comparison of hairpin versus linear capture probes. Nucleic acids research 29: 996-1004.
-
(2001)
Nucleic Acids Research
, vol.29
, pp. 996-1004
-
-
Riccelli, P.V.1
Merante, F.2
Leung, K.T.3
Bortolin, S.4
Zastawny, R.L.5
-
27
-
-
0035141632
-
Effect of polyethylene glycol on the supercoiling free energy of DNA
-
Naimushin A, Quach N, Fujimoto B, Schurr J, (2001) Effect of polyethylene glycol on the supercoiling free energy of DNA. Biopolymers 58: 204-217.
-
(2001)
Biopolymers
, vol.58
, pp. 204-217
-
-
Naimushin, A.1
Quach, N.2
Fujimoto, B.3
Schurr, J.4
-
28
-
-
0028817279
-
Real-time monitoring of DNA manipulations using biosensor technology
-
Nilsson P, Persson B, Uhlen M, Nygren P, (1995) Real-time monitoring of DNA manipulations using biosensor technology. Analytical Biochemistry 224: 400-408.
-
(1995)
Analytical Biochemistry
, vol.224
, pp. 400-408
-
-
Nilsson, P.1
Persson, B.2
Uhlen, M.3
Nygren, P.4
-
29
-
-
77956048370
-
Colour-barcoded magnetic microparticles for multiplexed bioassays
-
doi:10.1038/nmat2815
-
Lee H, Kim J, Kim H, Kim J, Kwon S, (2010) Colour-barcoded magnetic microparticles for multiplexed bioassays. Nature materials 9: 745-749 doi:10.1038/nmat2815.
-
(2010)
Nature Materials
, vol.9
, pp. 745-749
-
-
Lee, H.1
Kim, J.2
Kim, H.3
Kim, J.4
Kwon, S.5
-
31
-
-
34249854159
-
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
-
doi:10.1093/nar/gkm106
-
Geu-Flores F, Nour-Eldin HH, Nielsen MT, Halkier B a, (2007) USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products. Nucleic acids research 35: e55 doi:10.1093/nar/gkm106.
-
(2007)
Nucleic Acids Research
, vol.35
-
-
Geu-Flores, F.1
Nour-Eldin, H.H.2
Nielsen, M.T.3
Halkier, B.4
-
32
-
-
0343526348
-
High-throughput protein expression of cDNA products as a tool in functional genomics
-
Larsson M, Gräslund S, Yuan L, Brundell E, Uhlén M, et al. (2000) High-throughput protein expression of cDNA products as a tool in functional genomics. Journal of biotechnology 80: 143-157.
-
(2000)
Journal of Biotechnology
, vol.80
, pp. 143-157
-
-
Larsson, M.1
Gräslund, S.2
Yuan, L.3
Brundell, E.4
Uhlén, M.5
-
33
-
-
57049086900
-
Epitope mapping of antibodies using bacterial surface display
-
Rockberg J, Löfblom J, Hjelm B, Uhlen M, Ståhl S, (2008) Epitope mapping of antibodies using bacterial surface display. Nat Methods, 5, 1039-1045.
-
(2008)
Nat Methods
, vol.5
, pp. 1039-1045
-
-
Rockberg, J.1
Löfblom, J.2
Hjelm, B.3
Uhlen, M.4
Ståhl, S.5
-
34
-
-
0347915684
-
High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol
-
Wu SX, Letchworth GJ, (2004) High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. Biotechniques 36: 152-154.
-
(2004)
Biotechniques
, vol.36
, pp. 152-154
-
-
Wu, S.X.1
Letchworth, G.J.2
-
35
-
-
0031727275
-
A single-step screening procedure for Pichia pastoris clones by PCR
-
Biotecnologia Aplicada
-
Ayra-Pardo C, Martinez CG, de la Riva GA, (1998) A single-step screening procedure for Pichia pastoris clones by PCR. Biotecnologia Aplicada pp. 173-175.
-
(1998)
, pp. 173-175
-
-
Ayra-Pardo, C.1
Martinez, C.G.2
de la Riva, G.A.3
|