An experimental approach towards the development of an in vitro cortical-thalamic co-culture model

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

Volumn , Issue , 2011, Pages 648-651

  Kanagasabapathi, Thirukumaran T ^a   Massobrio, Paolo ^b   Tedesco, Mariateresa ^b   Martinoia, Sergio ^b,d   Wadman, Wytse J ^c   Decre, Michel M J ^a  

^a PHILIPS RESEARCH LABORATORIES   (Netherlands)

^b UNIVERSITY OF GENOA   (Italy)

^c ISTITUTO ITALIANO DI TECNOLOGIA   (Italy)

^d UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CO-CULTURE SYSTEM; COCULTURE; ELECTROPHYSIOLOGICAL RECORDINGS; EXPERIMENTAL APPROACHES; FIRING PATTERNS; IMMUNOFLUORESCENCE STAINING; IN-VITRO; INDIVIDUAL CELLS; NETWORK CONNECTIVITY; NEURITE GROWTH; NEURITES; PHYSICAL ISOLATION; PRELIMINARY ANALYSIS; SPONTANEOUS ACTIVITY;

ELECTROPHYSIOLOGY; MICROCHANNELS; NEURONS;

CELL CULTURE;

ANIMAL; ARTICLE; BRAIN CORTEX; CELL CULTURE; CELL PROLIFERATION; CELL SURVIVAL; COCULTURE; CYTOLOGY; EQUIPMENT; EQUIPMENT DESIGN; INSTRUMENTATION; MICROFLUIDIC ANALYSIS; NERVE TRACT; PHYSIOLOGY; RAT; THALAMUS; WISTAR RAT; EQUIPMENT FAILURE;

ANIMALS; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; CEREBRAL CORTEX; COCULTURE TECHNIQUES; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; MICROFLUIDIC ANALYTICAL TECHNIQUES; NEURAL PATHWAYS; RATS; RATS, WISTAR; THALAMUS;

EID: 84861952990     PISSN: 1557170X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/IEMBS.2011.6090144     Document Type: Conference Paper

References (10)

1. Nicolelis, M.A.L., Computing with thalamocortical ensembles during different behavioural states. Journal Of Physiology-London, 2005. 566(1): p. 37-47.
2. Magowan, G. and D.J. Price, Trophic and outgrowth-promoting effects of K+-induced depolarization on developing thalamic cells in organotypic culture. Neuroscience, 1996. 74(4): p. 1045-1057.
3. Asavaritikrai, P., et al., Regulation of cell survival in the developing thalamus: an in vitro analysis. Experimental Neurology, 2003. 181(1): p. 39-46.
4. Coronas, V., et al., Acetylcholine induces neuritic outgrowth in rat primary olfactory bulb cultures. Neuroscience, 2000. 98(2): p. 213-219.
5. Taylor, A.M., et al., Microfluidic multicompartment device for neuroscience research. Langmuir, 2003. 19(5): p. 1551-1556.
6. Kanagasabapathi, T., et al. Establishing functional connectivity in partly segregated neuronal cell cultures. in Proceedings of Society for Neuroscience 2010, 40th annual meeting. 2010.
7. Kanagasabapathi, T.T., et al., Dual compartment neurofluidic system for electrophysiological measurements in physically segregated and functionally connected neuronal cell culture. Lab On A Chip, in press.
8. Bologna, L.L., et al., Investigating neuronal activity by SPYCODE multi-channel data analyzer. Neural Networks. 23(6): p. 685-697.
9. Maccione, A., et al., A novel algorithm for precise identification of spikes in extracellularly recorded neuronal signals. Journal Of Neuroscience Methods, 2009. 177(1): p. 241-249.
10. Chiappalone, M., et al., Networks of neurons coupled to microelectrode arrays: a neuronal sensory system for pharmacological applications. Biosensors & Bioelectronics, 2003. 18(5-6): p. 627-634.