Heat-induced antigen retrieval applied in zebrafish: Whole-mount in situ immunofluorescence microscopy

Microscopy and Microanalysis

Volumn 18, Issue 3, 2012, Pages 493-496

  Lin, Chuang Yu ^a   Su, Wen Ta ^a   Li, Li Tzu ^b  

^a NATIONAL TAIPEI UNIVERSITY OF TECHNOLOGY   (Taiwan)

^b INSTITUTE OF CELLULAR AND ORGANISMIC BIOLOGY   (Taiwan)

Author keywords

antigen retrieval; fluorescence quantification; immunofluorescence microscopy; whole mount in situ

Indexed keywords

ANTIGEN; PROTEIN;

ANIMAL; ARTICLE; FLUORESCENCE MICROSCOPY; HEAT; METHODOLOGY; TISSUE FIXATION; ZEBRA FISH;

ANIMALS; ANTIGENS; HOT TEMPERATURE; MICROSCOPY, FLUORESCENCE; PROTEINS; TISSUE FIXATION; ZEBRAFISH;

DANIO RERIO;

EID: 84861900282     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927612000141     Document Type: Article

References (13)

1. Borman, W.H. & Yorde, D.E. (1994). Analysis of chick somite myogenesis by in-situ confocal microscopy of desmin expression. J Histochem Cytochem 42 (2), 265-272. (Pubitemid 24042243)
2. Cox, M.L., Schray, C.L., Luster, C.N., Stewart, Z.S., Korytko, P.J., Khan, K.N.M., Paulauskis, J.D. & Dunstan, R.W. (2006). Assessment of fixatives, fixation, and tissue processing on morphology and RNA integrity. Exp Molec Pathol 80 (2), 183-191.
3. Fassel, T.,Mozdziak, P.E., Sanger, J.R. & Edmiston, C.E. (1998). Superior preservation of the staphylococcal glycocalyx with aldehyde ruthenium red and select lysine salts using extended fixation times. Microsc Res Techniq 41 (4), 291-297. (Pubitemid 28264610)
4. Hess, M.W., Klima, G., Pfaller, K., Kunzel, K.H. & Gaber, O. (1998). Histological investigations on the Tyrolean Ice Man. Am J Phys Anthropol 106 (4), 521-532. (Pubitemid 28367202)
5. Inoue, D. & Wittbrodt, J. (2011). One for all-A highly efficient and versatile method for fluorescent immunostaining in fish embryos. Plos One 6 (5), e19713.
6. Lambot, M.A.H., Mendive, F., Laurent, P., Van Schoore, G., Noel, J.C., Vanderhaeghen, P. & Vassart, G. (2009). Threedimensional reconstruction of efferent ducts in wild-type and Lgr4 knock-out mice. Anat Rec (Hoboken) 292 (4), 595-603.
7. Otali, D., Stockard, C.R., Oelschlager, D.K.,Wan,W.,Manne, U., Watts, S.A. & Grizzle, W.E. (2009). Combined effects of formalin fixation and tissue processing on immunorecognition. Biotechnic Histochem 84 (5), 223-247.
8. Paavilainen, L., Edvinsson, A., Asplund, A., Hober, S., Kampf, C., Ponten, F. & Wester, K. (2010). The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells. J Histochem Cytochem 58 (3), 237-246.
9. Pavlakis, E. & Chalepakis, G. (2008). pH-dependent antigen unmasking in paraformaldehyde-fixed tissue cryosections. Appl Immunohistochem Molec Morphol 16 (5), 503-506.
10. Piaton, E., Faynel, J., Ruffion, A., Lopez, J., Perrin, P. & Devonec, M. (2005). p53 immunodetection of liquid-based processed urinary samples helps to identify bladder tumours with a higher risk of progression. Brit J Cancer 93 (2), 242-247. (Pubitemid 41133211)
11. Shimada, A., Shibata, T., Komatsu, K. & Nifuji, A. (2008). Improved methods for immunohistochemical detection of BrdU in hard tissue. J Immunol Methods 339 (1), 11-16.
12. Vassallo, J., Pinto, G.A., Alvarenga, M., Zeferino, L.C., Chagas, C.A. & Metze, K. (2004). Comparison of immunoexpression of 2 antibodies for estrogen receptors (1D5 and 6F11) inbreast carcinomas using different antigen retrieval and detection methods. Appl Immunohistochem Molec Morphol 12 (2), 177-182. (Pubitemid 38796461)
13. Wheeler, G.N. & Brandli, A.W. (2009). Simple vertebrate models for chemical genetics and drug discovery screens: Lessons from zebrafish and xenopus. Develop Dynam 238 (6), 1287-1308.