A particle-enhanced double-stranded DNA probe for rapid detection of bacterial 16S rRNA toward urinary tract infection diagnostics

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Volumn 3, Issue , 2011, Pages 1944-1946

  Riahi, Reza ^a   Liao, Joseph C ^b   Wong, Pak Kin ^a  

^a UNIVERSITY OF ARIZONA   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

Bacterial 16S rRNA; dsDNA probe; Microparticle; Sample preparation

Indexed keywords

16S RRNA; DOUBLE STRANDED DNA; DOUBLE-STRANDED DNA (DS-DNA); MICRO PARTICLES; POINT-OF-CARE DIAGNOSTICS; SAMPLE PREPARATION; THREE ORDERS OF MAGNITUDE; URINARY TRACT INFECTIONS;

RNA;

PROBES;

EID: 84861838992     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (6)

1. D. Meserve, Z. Wang, D.D. Zhang, and P.K. Wong, "A double-stranded molecular probe for homogeneous nucleic acid analysis,", Analyst, 133, pp. 1013-1019, (2008).
2. V. Gidwani, R. Riahi, D.D. Zhang, and P.K. Wong, "Hybridization kinetics of double-stranded DNA probes for rapid molecular analysis,", Analyst, 134, pp. 1675-1681, (2009).
3. A.O. Crockett, and C.T. Wittwer, "Fluorescein-labeled oligonucleotides for real-time PCR: Using the inherent quenching of deoxyguanosine nucleotides,", Analytical Biochemistry, 290, pp. 89-97, (2001).
4. M.F. Ali, R. Kirby, A.P. Goodey, M.D. Rodriguez, A.D. Ellington, D.P. Neikirk, and J.T. McDevitt, "DNA hybridization and discrimination of single-nucleotide mismatches using chip-based microbead arrays,", Analytical Chemistry, 75, pp. 4732-4739, (2003).
5. D. Horejsh, F. Martini, F. Poccia, G. Ippolito, A. Di Caro, and M.R. Capobianchi, "A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry,", Nucleic Acids Res, 33, e13, (2005).
6. J.C. Liao, M. Mastali, V. Gau, M.A. Suchard, A.K. Moller, D.A. Bruckner, J.T. Babbitt, Y. Li, J. Gornbein, E.M. Lan-daw, "Use of electrochemical DNA biosensors for rapid molecular identification of uropathogens in clinical urine specimens,", J Clin Microbiol, 44, pp. 561-570, (2006).