The role of femA regulating gene on methicillin-resistant Staphylococcus aureus clinical isolates

Medecine et Maladies Infectieuses

Volumn 42, Issue 5, 2012, Pages 218-225

  Li, X ^a   Xiong, Y ^a   Fan, X ^a   Feng, P ^a   Tang, H ^a   Zhou, T ^a  

^a Digestive System Department   (China)

Author keywords

FemA; MRSA; Regulating gene

Indexed keywords

BETA LACTAMASE; MESSENGER RNA; OXACILLIN; PENICILLIN BINDING PROTEIN 2A;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CONTROLLED STUDY; ENZYME SYNTHESIS; FEMA GENE; GEL MOBILITY SHIFT ASSAY; GENE AMPLIFICATION; GENE EXPRESSION; GENE FUNCTION; GENE SEQUENCE; MECA GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SUSCEPTIBLE STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PROTEIN EXPRESSION; REAL TIME FLUORESCENT QUANTITATIVE POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; REGULATOR GENE;

BACTERIAL PROTEINS; BASE SEQUENCE; CELL WALL; DNA, BACTERIAL; ELECTROPHORETIC MOBILITY SHIFT ASSAY; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, REGULATOR; HUMANS; METHICILLIN RESISTANCE; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR SEQUENCE DATA; REAL-TIME POLYMERASE CHAIN REACTION; REGULATORY SEQUENCES, NUCLEIC ACID; RNA, BACTERIAL; RNA, MESSENGER; STAPHYLOCOCCAL INFECTIONS;

EID: 84861827479     PISSN: 0399077X     EISSN: 17696690     Source Type: Journal    
DOI: 10.1016/j.medmal.2008.09.031     Document Type: Article

References (21)

1. Georgopapadakou N.H., Smith S.A., Bonner D.P. Penicillin-binding proteins in a Staphylococcus aureus strain resistant to specific beta-lactam antibiotics. Antimicrob Agents Chemother 1982, 22:172-175.
2. Berger-Bachi B., Barberis-Maino L., Strassle A., Kayser F.H. FemA. a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization. Mol Gen Genet 1989, 219:263-269.
3. Ton That H., Labischinski H., Berger-Bachi B., Schneewind O. Anchor structure of Staphylococcus surface proteins. Role of the femA, femB and femX factors in anchoring surface proteins to the bacterial cell wall. J Biol Chem 1998, 273:29143-29149.
4. Chen Z., Fan X., Lu X. Combination PCR of mecA, femA genes for detection of MRSA. Sichuan Da Xue Xue Bao Yi Xue Ban 2003, 34:663-666.
5. Krishnan P.U., Miles K., Shetty N. Detection of methicillin and mupirocin resistance in Staphylococcus aureus isolates using conventional and molecular methods. J Clin Pathol 2002, 55:745-748.
6. Hackbarth C.J., Chambers H.F. blaI and blaR1 regulate β-lactamase and PBP2a production in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1993, 37:1144-1147.
7. Hegde S.S., Shrader T.E. FemABX family members are novel ribosomal peptidyltransferases and important pathogen-specific drugs targets. J Biol Chem 2001, 276:6998-7002.
8. MIC Interpretive Standards for Staphylococcus. National Committee for Clinical Laboratory Standard, MIC testing Supplemental Tables, 2004; 20-24.
9. Christiane G., Silvia C., Bayer M.G. Direct quantitative transcript analysis of the agr regulation of Staphylococcus aureus during human infection in comparison to the expression profile in vitro. Infect Immun 2000, 68:1304-1311.
10. Rosato A.E., Craig W.A., Archer G.L. Quantization of mecA transcription in oxacillin- resistant staphylococcus aureus clinical isolates. J Bacteriol 2003, 185:3446-3452.
11. Murakami K., Tomasz A. Involvement of multiple genetic determinants in high-level methicillin resistance in Staphylococcus aureus. J Bacteriol 1989, 171:874-879.
12. De Lencastre H., Wu S.W., Pinho M.G., Ludovice A.M. Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin. Microb Drug Resist Mech Epidemiol Dis 2000, 5:163-175.
13. Maidhof H., Reinicke B., Blumel P., Berger-Bachi B. femA, which encodes a factor essential for expression of methicillin resistance, affects glycine content of peptidoglycan in methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains. J Bacteriol 1991, 173:3507-3513.
14. Hurlimann-Dalel R., Ryffel C., Kayser F. Survey of the methicillin resistance-associated genes mecA, mecR-mecI, and femA-femB in clinical isolates of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1992, 36:2617-2621.
15. Alborn W., Hoskins J., Flokowitsh J. Cloning and characterization of femA and femB from Staphylococcus epidermidis and Staphylococcus haemolytica. Program and Abstracts of the 34th Interscience Conference on Antimicrobial Agents and Chemotherapy 1994, C59. American Society for Microbiology, Washington DC.
16. Stranden A.M., Ehlert K., Labishinski H. Cell wall monoglycine cross-bridges and methicillin hypersusceptibility in a femAB null mutant of methicillin resistance Staphylococcus aureus. J Bacteriol 1997, 179:9-12.
17. Tschierske M., Mori C., Rohrer S., Ehlert K., Shaw K.J., Berger-Bachi B. Identification of three additional femAB-like open reading frames in Staphylococcus aureus. FEMS Microbiol Lett 1994, 171:97-102.
18. Kobayshi N., Wu H., Kojima K. Detection of mecA, femA, and femB genes in clinical strains of staphylococci using polymerase chain reaction. Epidermiol Infect 1994, 11:259-264.
19. Alborn W.E., Hoskins J., Unal S. Cloning and characterization of femA and femB from Staphylococcus epidermidis. Gene 1996, 180:177-184.
20. Berger-Bachi B., Kohler M. A novel site on the chromosome of Staphylococcus aureus influencing the level of methicillin resistance: genetic mapping. FEMS Microbiol Lett 1983, 20:305-308.
21. Pitkälä A., Salmikivi L., Bredbacka P., Myllyniemi A.-L., Koskinen M.T. Comparison of Tests for Detection of β-Lactamase-Producing Staphylococci. J Clin Microbiol 2007, 45:2031-2033.