Cloning and characterization of femA and femB from Staphylococcus epidermidis

Gene

Volumn 180, Issue 1-2, 1996, Pages 177-181

  Alborn Jr , William E ^a   Hoskins, JoAnn ^a   Ünal, Serhat ^b   Flokowitsch, Jane E ^a   Hayes, Carolyn A ^a   Dotzlaf, Joe E ^a   Yeh, W K ^a   Skatrud, Paul L ^a  

^a ELI LILLY AND COMPANY   (United States)

^b HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Cloning; FEMA purification; Methicillin resistance; Nucleotide sequencing

Indexed keywords

DNA FRAGMENT;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA SEQUENCE; ESCHERICHIA COLI; GENE DUPLICATION; GENETIC ORGANIZATION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN PURIFICATION; STAPHYLOCOCCUS EPIDERMIDIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STAPHYLOCOCCUS EPIDERMIDIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 0030597358     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00450-7     Document Type: Article

References (17)

1. Berger-Bächi, B., Barberis-Maino, L., Strässle, A. and Kayser, F.H. (1989) femA, a host mediated factor essential for methicillin resistance in S. aureus: molecular cloning and characterization. Mol. Gen. Genet. 219, 263-269.
2. Berger-Bächi, B., Strässle, A., Gustafson, J.E. and Kayser, F.H. (1992) Mapping and characterization of multiple chromosomal factors involved in methicillin resistance in S. aureus. Antimicrob. Agents Chemother. 36, 1367-1373.
3. Chambers, H.F. and Hackbarth, C.J. (1987) Effect of NaCl and nafcillin on penicillin-binding protein 2a and heterogeneous expression of methicillin resistance in S. aureus. Antimicrob. Agents Chemother. 31, 1982-1988.
4. De Jonge, B.L.M., De Lencastre, H. and Tomasz, A. (1991) Suppression of autolysis and cell wall turnover in heterogeneous Tn551 mutants of methicillin-resistant S. aureus. J. Bacteriol. 173, 1105-1110.
5. Hartman, B.J. and Tomasz, A. (1984) Low-affinity penicillin-binding protein associated with β-lactam resistance in S. aureus. J. Bacteriol. 158, 513-516.
6. Henze U., Sidow, T., Wecke, J., Labischinski, H. and Berger-Bächi, B. (1993) Influence of femB on methicillin resistance and peptidoglycan metabolism in S. aureus. J. Bacteriol. 175, 1612-1620.
7. Kobayashi, Y., Kizaki, M., Nakajima, H., Mori, S., Kawakami, Y., Uchida, H. and Ikeda, Y. (1992) Detection of genes for mecA, femA, and TSST-1 in staphylocci isolated from blood by polymerase chain reaction. Abstract #701 of the 1992 Annual ICAAC Meeting, p. 227
8. Kornblum, J., Hartman, B.J., Novick, R.P. and Tomasz, A. (1986) Conversion of a homogeneously methicillin-resistant strain of S. aureus to heterogeneous resistance by Tn551-mediated insertional inactivation. Eur. J. Clin. Microbiol. 5, 714-718.
9. Labischinski, H. (1992) Consequences of the interaction of β-lactam antibiotics with penicillin-binding proteins from sensitive and resistant S. aureus strains. Med. Microbiol. Immunol. 181, 241-265.
10. Maidhof, H., Reinicke, B., Blümel, P., Berger-Bächi, B. and Labischinski, H. (1991) femA, which encodes a factor essential for expression of methicillin resistance, affects glycine content of peptidoglycan in methicillin-resistant and methicillin-susceptible S. aureus strains. J. Bacteriol. 173, 3507-3513.
11. Murakami, K. and Tomasz, A. (1989) Involvement of multiple genetic determinants in high-level methicillin resistance in S. aureus. J. Bacteriol. 171, 874-879.
12. Ryffel, C., Tesch, W., Birch-Machin, I., Reynolds, P.E., Barberis-Maino, L., Kayser, F.H. and Berger-Bächi, B. (1990) Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Gene 94, 137-138.
13. Ryffel, C., Strässle, A., Kayser, F.H., and Berger-Bächi, B. (1994) Mechanisms of heteroresistance in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 38(4), 724-728.
14. Song, M.D., Wachi, M., Doi, M., Ishino, F. and Matsuhashi, M. (1987) Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Lett. 221, 167-171.
15. Tomasz, A. (1990) Auxiliary genes assisting in the expression of methicillin resistance in S. aureus. In: Novick, R.P. (Ed.), Molecular Biology of the Staphylococci, VCH Publishers, New York, pp. 565-584.
16. Ünal, S., Hoskins, J., Flokowitsch, J.E., Wu, C.Y.E., Preston, D.A. and Skatrud, P.L. (1992) Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. J. Clin. Microbiol. 30, 1685-1691.
17. Wu, C.Y.E., Hoskins, J., Blaszczak, L.C., Preston, D.A. and Skatrud, P.L. (1992) Construction of a water-soluble form of penicillin-binding protein 2a from a methicillin-resistant S. aureus isolate. Antimicrob. Agents Chemother. 36, 533-539.