Virus-induced silencing of genes encoding LEA protein in Tibetan hulless barley (Hordeum vulgare ssp. vulgare) and their relationship to drought tolerance

Molecular Breeding

Volumn 30, Issue 1, 2012, Pages 441-451

  Liang, Junjun ^a,b   Deng, Guangbing ^a   Long, Hai ^a   Pan, Zhifen ^a   Wang, Chunping ^a   Cai, Peng ^c,d   Xu, Deling ^a,b   Nima, Zha Xi ^e,f   Yu, Maoqun ^a  

^a CHENGDU INSTITUTE OF BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c SICHUAN AGRICULTURAL UNIVERSITY   (China)

^d CHINA WEST NORMAL UNIVERSITY   (China)

^e Tibet Academy of Agriculture and Animal Sciences   (China)

^f Barley Improvement and Yak Breeding Key Lab of Tibet Autonomous Region   (China)

Author keywords

Barley stripe mosaic virus (BSMV); Dhn6; HVA1; Virus induced gene silencing (VIGS); Water loss rate (WLR)

Indexed keywords

AGRONOMY; DROUGHT; ECOLOGY; GENE ENCODING; GENE EXPRESSION; GENETIC ENGINEERING; PLANTS (BOTANY); POLYMERASE CHAIN REACTION;

BARLEY STRIPE MOSAIC VIRUS; DHN6; HULLESS BARLEIES; HVA1; MOSAIC VIRUS; TIBETANS; VIRUS-INDUCED GENE SILENCING; WATER LOSS RATE; WATER LOSS RATES;

VIRUSES;

BARLEY STRIPE MOSAIC VIRUS; HORDEUM; HORDEUM VULGARE;

EID: 84861757904     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11032-011-9633-3     Document Type: Article

References (56)

1. Abba S, Ghignone S, Bonfante P (2006) A dehydration-inducible gene in the truffle Tuber borchii identifies a novel group of dehydrins. BMC Genomics 7: 39-53. doi: 10. 1186/1471-2164-7-39.
2. Araus JL, Slafer GA, Reynolds MP, Royo C (2002) Plant breeding and drought in C-3 cereals: what should we breed for? Ann Bot 89: 925-940. doi: 10. 1093/aob/mcf049.
3. Babu RC, Zhang J, Blum A, Ho THD, Wu R, Nguyen HT (2004) HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection. Plant Sci 166: 855-862. doi: 10. 1016/j. plantsci. 2003. 11. 023.
4. Bahieldin A, Mahfouz HT, Eissa HF, Saleh OM, Ramadan AM, Ahmed IA, Dyer WE, El-Itriby HA, Madkour MA (2005) Field evaluation of transgenic wheat plants stably expressing the HVA1 gene for drought tolerance. Physiol Plant 123: 421-427. doi: 10. 1111/j. 1399-3054. 2005. 00470. x.
5. Baulcombe DC (1999) Fast forward genetics based on virus induced gene silencing. Curr Opin Plant Biol 2: 109-113. doi: 10. 1016/S1369-5266(99)80022-3.
6. Bies-Ethève N, Gaubier-Comella P, Debures A, Lasserre E, Jobet E, Raynal M, Cooke R, Delseny M (2008) Inventory, evolution and expression profiling diversity of the LEA (late embryogenesis abundant) protein gene family in Arabidopsis thaliana. Plant Mol Biol 67: 107-124. doi: 10. 1007/s11103-008-9304-x.
7. Bray EA (1997) Plant responses to water deficit. Trends Plant Sci 2: 48-54. doi: 10. 1016/S1360-1385(97)82562-9.
8. Bray EA, Bailey-Serres J, Weretilnyk E (2000) Responses to abiotic stresses. In: Gruissem W, Buchannan B, Jones R (eds) Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville, pp 1158-1249.
9. Browne JA, Dolan KM, Tyson T, Goyal K, Tunnacliffe A, Burnell AM (2004) Dehydration-specific induction of hydrophilic protein genes in the anhydrobiotic nematode Aphelenchus avenae. Eukaryot Cell 3: 966-975. doi: 10. 1128/EC. 3. 4. 966-975. 2004.
10. Burch-Smith TM, Anderson JC, Martin GB, Dinesh-Kumar SP (2004) Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J 39: 734-746. doi: 10. 1111/j. 1365-313X. 2004. 02158. x.
11. Campbell SA, Close TJ (1997) Dehydrins: genes, proteins, and associations with phenotypic traits. New Phytol 137: 61-74. doi: 10. 1046/j. 1469-8137. 1997. 00831. x.
12. Chandler PM, Robertson M (1994) Gene expression regulated by abscisic acid and its relation to stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 45: 113-141. doi: 10. 1146/annurev. pp. 45. 060194. 000553.
13. Chauhan H, Khurana P (2011) Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics. Plant Biotechnol J 9: 408-417. doi: 10. 1111/j. 1467-7652. 2010. 00561. x.
14. Clarke JM, McCaig TN (1982) Evaluation of techniques for screening for drought resistance in wheat. Crop Sci 22: 503-506. doi: 10. 2135/cropsci1982. 0011183X002200030015x.
15. Dalal M, Tayal D, Chinnusamy V, Bansal KC (2009) Abiotic stress and ABA-inducible group 4 LEA from Brassica napus plays a key role in salt and drought tolerance. J Biotechnol 139: 137-145. doi: 10. 1016/j. jbiotec. 2008. 09. 014.
16. Demmig-Adams B, Adams WW III (1992) Photoprotection and other responses of plants to high light stress. Annu Rev Plant Physiol Plant Mol Biol 43: 599-626. doi: 10. 1146/annurev. pp. 43. 060192. 003123.
17. Dombrowski JE, Martin RC (2009) Evaluation of reference genes for quantitative RT-PCR in Lolium temulentum under abiotic stress. Plant Sci 176: 390-396. doi: 10. 1016/j. plantsci. 2008. 12. 005.
18. Dure L III, Chlan C (1981) Developmental biochemistry of cottonseed embryogenesis and germination. XII. Purification and properties of principal storage proteins. Plant Physiol 68: 180-186. doi: 10. 1104/pp. 68. 1. 180.
19. Dure L III, Galau GA (1981) Developmental biochemistry of cottonseed embryogenesis and germination. XIII. Regulation of biosynthesis of principal storage proteins. Plant Physiol 68: 187-194. doi: 10. 1104/pp. 68. 1. 187.
20. Dure L III, Greenway SC, Galau GA (1981) Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis. Biochemistry 20: 4162-4168. doi: 10. 1021/bi00517a033.
21. Dure L III, Crouch M, Harada J, Ho THD, Mundy J, Quatrano R, Thomas T, Sung ZR (1989) Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol 12: 475-486. doi: 10. 1007/BF00036962.
22. Fu D, Huang B, Xiao Y, Muthukrishnan S, Liang GH (2007) Overexpression of barley HVA1 gene in creeping bentgrass for improving drought tolerance. Plant Cell Rep 26: 467-477. doi: 10. 1007/s00299-006-0258-7.
23. Garay-Arroyo A, Colmenero-Flores JM, Garciarrubio A, Covarrubias AA (2000) Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. J Biol Chem 275: 5668-5674. doi: 10. 1074/jbc. 275. 8. 5668.
24. Godge MR, Purkayastha A, Dasgupta I, Kumar PP (2008) Virus-induced gene silencing for functional analysis of selected genes. Plant Cell Rep 27: 209-219. doi: 10. 1007/s00299-007-0460-2.
25. Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J (2009) Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. J Exp Bot 60(12): 3531-3544. doi: 10. 1093/jxb/erp194.
26. Hartl M, Merker H, Schmidt DD, Baldwin IT (2008) Optimized virus-induced gene silencing in Solanum nigrum reveals the defensive function of leucine aminopeptidase against herbivores and the shortcomings of empty vector controls. New Phytol 179: 356-365. doi: 10. 1111/j. 1469-8137. 2008. 02479. x.
27. Holzberg S, Brosio P, Gross C, Pogue GP (2002) Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J 30: 315-327. doi: 10. 1046/j. 1365-313X. 2002. 01291. x.
28. Hundertmark M, Hincha DK (2008) LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genomics 9: 118-139. doi: 10. 1186/1471-2164-9-118.
29. Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol 47: 377-403. doi: 10. 1146/annurev. arplant. 47. 1. 377.
30. Kumagai MH, Donson J, Della-Cioppa G, Harvey D, Hanley K, Grill LK (1995) Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci USA 92: 1679-1683. doi: 10. 1073/pnas. 92. 5. 1679.
31. Lal S, Gulyani V, Khurana P (2008) Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica). Transgenic Res 17: 651-663. doi: 10. 1007/s11248-007-9145-4.
32. Liu Y, Schiff M, Dinesh-Kumar SP (2002) Virus-induced gene silencing in tomato. Plant J 31: 777-786. doi: 10. 1046/j. 1365-313X. 2002. 01394. x.
33. Long XY, Wang JR, Ouellet T, Rocheleau H, Wei YM, Pu ZE, Jiang QT, Lan XJ, Zheng YL (2010) Genome-wide identification and evaluation of novel internal control genes for Q-PCR based transcript normalization in wheat. Plant Mol Biol 74: 307-311. doi: 10. 1007/s11103-010-9666-8.
34. Mtwisha L, Brandt W, McCready S, Lindsey GG (1998) HSP 12 is a LEA-like protein in Saccharomyces cerevisiae. Plant Mol Biol 37: 513-521. doi: 10. 1023/A: 1005904219201.
35. NDong C, Danyluk J, Wilson KE, Pocock T, Huner NP, Sarhan F (2002) Cold-regulated cereal chloroplast late embryogenesis abundant-like proteins: molecular characterization and functional analyses. Plant Physiol 129: 1368-1381. doi: 10. 1104/pp. 001925.
36. Nicot N, Hausman JF, Hoffmann L, Evers D (2005) Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 56(421): 2907-2914. doi: 10. 1093/jxb/eri285.
37. Olvera-Carrillo Y, Campos F, Reyes JL, Garciarrubio A, Covarrubias AA (2010) Functional analysis of the group 4 late embryogenesis abundant proteins reveals their relevance in the adaptive response during water deficit in Arabidopsis. Plant Physiol 154: 373-390. doi: 10. 1104/pp. 110. 158964.
38. Puhakainen T, Hess MW, Makela P, Svensson J, Heino P, Palva ET (2004) Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis. Plant Mol Biol 54: 743-753. doi: 10. 1023/B: PLAN. 0000040903. 66496. a4.
39. Qian G (2007) Genotypic variability in sequences and expression of LEA2/LEA3 genes in Tibetan Hulless Barley, Hordeum vulgare ssp. Vulgare, associated with resistance to water deficit. Dissertation, Chengdu Institute of Biology, the Chinese Academy of Sciences.
40. Qian G, Han ZX, Zhao T, Deng GB, Pan ZF, Yu MQ (2007) Genotypic variability in sequence and expression of HVA1 gene in Tibetan hulless barley, Hordeum vulgare ssp. vulgare, associated with resistance to water deficit. Aust J Agric Res 58: 425-431. doi: 10. 1071/AR06300.
41. Ratcliff F, Harrison BD, Baulcombe DC (1997) A similarity between viral defense and gene silencing in plants. Science 276: 1558-1560. doi: 10. 1126/science. 276. 5318. 1558.
42. Ristic Z, Jenks MA (2002) Leaf cuticle and water loss in maize lines differing in dehydration avoidance. J Plant Physiol 159: 645-651. doi: 10. 1078/0176-1617-0743.
43. Robertson M, Chandler PM (1994) A dehydrin cognate protein from pea (Pisum sativum L.) with an atypical pattern of expression. Plant Mol Biol 26: 805-816. doi: 10. 1007/BF00028850.
44. Rohila JS, Jain RK, Wu R (2002) Genetic improvement of Basmati rice for salt and drought tolerance by regulated expression of a barley Hva1 cDNA. Plant Sci 163: 525-532. doi: 10. 1016/S0168-9452(02)00155-3.
45. Seckler D, Amarasinghe U, Molden D, de Silva R, Barker R (1998) World water demand and supply, 1990 to 2025: scenarios and issues. Colombo, Sri Lanka: International Water Management Institute (IIMI); IWMI. vi, 40p. (IIMI Research Report 19/IWMI Research Report 19). doi: 10. 3910/2009. 019.
46. Seckler D, Barker R, Amarasinghe UA (1999) Water scarcity in the twenty-first century. Int J Water Resour Dev 15: 29-42. doi: 10. 1080/07900629948916.
47. Sivamani E, Bahieldin A, Wraith JM, Al-Niemi T, Dyer WE, Ho THD, Qu R (2000) Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene. Plant Sci 155: 1-9. doi: 10. 1016/S0168-9452(99)00247-2.
48. Solomon A, Salomon R, Paperna I, Glazer I (2000) Desiccation stress of entomopathogenic nematodes induces the accumulation of a novel heat-stable protein. Parasitology 121: 409-416. doi: 10. 1017/S0031182099006563.
49. Stacy RAP, Aalen RB (1998) Identification of sequence homology between the internal hydrophilic repeated motifs of group 1 late-embryogenesis-abundant proteins in plants and hydrophilic repeats of the general stress protein GsiB of Bacillus subtilis. Planta 206: 476-478. doi: 10. 1007/s004250050424.
50. Suppunova T, Krugman T, Fahima T, Chen G, Shams I, Korol A, Nevo E (2004) Differential expression of dehydrin genes in wild barley, Hordeum spontaneum, associated with resistance to water deficit. Plant Cell Environ 27: 1297-1308. doi: 10. 1111/j. 1365-3040. 2004. 01237. x.
51. Thomashow MF (1998) Role of cold-responsive genes in plant freezing tolerance. Plant Physiol 118: 1-8. doi: 10. 1104/pp. 118. 1. 1.
52. Wan H, Zhao Z, Qian C, Sui Y, Malik AA, Chen J (2010) Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber. Anal Biochem 399: 257-261. doi: 10. 1016/j. ab. 2009. 12. 008.
53. Wu CJ, Jia LL, Goggin F (2011) The reliability of virus-induced gene silencing experiments using tobacco rattle virus in tomato is influenced by the size of the vector control. Mol Plant Pathol 12(3): 299-305. doi: 10. 1111/J. 1364-3703. 2010. 00669. X.
54. Xiao B, Huang Y, Tang N, Xiong L (2007) Over-expression of a LEA gene in rice improves drought resistance under the field conditions. Theor Appl Genet 115: 35-46. doi: 10. 1007/s00122-007-0538-9.
55. Xu D, Duan X, Wang B, Hong B, Ho T-HD, Wu R (1996) Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol 110: 249-257. doi: 10. 1104/pp. 110. 1. 249.
56. Yang GF, Peng HX, Li CA, Yin HF (2001) Analysis of hydrothermal factors and presentation of ecological recovery measures in Northwest China. Northwest Geol 34: 9-15. doi: cnki: SCN: 61-1174. 0. 2001-04-00.