Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Selvin, Joseph ^a   Kennedy, Jonathan ^b   Lejon, David P H ^b,c   Kiran, G S ^d   Dobson, Alan D W ^b  

^a BHARATHIDASAN UNIVERSITY   (India)

^b UNIVERSITY COLLEGE CORK   (Ireland)

^c UNIV GRENOBLE ALPES   (France)

^d PONDICHERRY UNIVERSITY   (India)

Author keywords

Functional screening; Lipase; Marine sponge; Metagenomic library

Indexed keywords

AMINO ACID; BETA LACTAMASE; HALO TOLERANT LIPASE; LONG CHAIN FATTY ACID; PALMITIC ACID DERIVATIVE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; SODIUM CHLORIDE; TRIACYLGLYCEROL LIPASE; TRIBUTYRIN; UNCLASSIFIED DRUG;

ARTICLE; BIOCHEMISTRY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; GENE SEQUENCE; HALICLONA; HALICLONA SIMULANS; HETEROLOGOUS EXPRESSION; HIGH THROUGHPUT SCREENING; ISOLATION PROCEDURE; METAGENOME; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PH; PHYLOGENETIC TREE; TEMPERATURE;

AMINO ACID SEQUENCE; ANIMALS; CALCIUM; CLONING, MOLECULAR; HALICLONA; HYDROGEN-ION CONCENTRATION; IONS; LIPASE; METAGENOME; METALS; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLASMIDS; PROTEIN STABILITY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SODIUM CHLORIDE; SUBSTRATE SPECIFICITY; TEMPERATURE;

HALICLONA SIMULANS;

EID: 84861716212     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-72     Document Type: Article

References (38)

1. Siddiqui KS, Cavicchioli R. Cold-adapted enzymes. Annu Rev Biochem 2006, 75:403-433. 10.1146/annurev.biochem.75.103004.142723, 16756497.
2. Trincone A. Marine Biocatalysts- Enzymatic Features and Applications. Mar Drugs 2011, 9:478-499. 10.3390/md9040478, 3124967, 21731544.
3. Webster NS. Taylor: Marine sponges and their microbial symbionts: love and other relationships. Environ Microbiol 2011, [Epub ahead of print].
4. Wilkinson CR. Microbial association in sponges II: numerical analysis of sponge and water bacterial populations. Mar Biol 1978, 49:169-176.
5. Kennedy J, Leary ND, Kiran GS, Morrissey JP, Gara F, Selvin J, Dobson ADW. Functional metagenomic strategies for the discovery of novel enzymes and biosurfactants with biotechnological applications from marine ecosystems. J Appl Microbiol 2011, 111:787-799. 10.1111/j.1365-2672.2011.05106.x, 21777355.
6. Amann RI, Ludwig W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 1995, 59(1):143-169. 239358, 7535888.
7. Kennedy J, Marchesi JR, Dobson ADW. Marine metagenomics: strategies for the discovery of novel enzymes with biotechnological applications from marine environments. Microb Cell Fact 2008, 7:2. 10.1186/1475-2859-7-2, 2275215, 18257924.
8. Simon C, Daniel R. Metagenomic analyses: past and future needs. Appl Environ Microbiol 2011, 77:1153-1161. 10.1128/AEM.02345-10, 3067235, 21169428.
9. Ferrer M, Beloqui A, Timmis KN, Golyshin PN. Metagenomics for mining new genetic resources of microbial communities. J Mol Microbiol Biotechnol 2009, 16:109-123. 10.1159/000142898, 18957866.
10. Steele HL, Jaeger KE, Daniel R, Streit W. Advances in recovery of novel biocatalysts from metagenomes. J Mol Microbiol Biotechnol 2009, 16:25-37. 10.1159/000142892, 18957860.
11. Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D, Eisen JA, Wu D, Paulsen I, Nelson KE, Nelson W, et al. Environmental genome shotgun sequencing of the Sargasso Sea. Science 2004, 304:66-74. 10.1126/science.1093857, 15001713.
12. Jaeger KE, Eggert T. Lipases for biotechnology. Curr Opin Biotechnol 2002, 13:390-397. 10.1016/S0958-1669(02)00341-5, 12323363.
13. Arpigny JL, Jaeger KE. Bacterial lipolytic enzymes: classification and properties, Biochem. J. 1999, 343:177-183.
14. Henne A, Schmitz RA, Bomeke M, Gottschalk G. Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli. Appl Environ Microbiol 2000, 66:3113-3116. 10.1128/AEM.66.7.3113-3116.2000, 92121, 10877816.
15. Lee MH, Lee CH, Oh TK, Song JK, Yoon JH. Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacteria lipases. Appl Environ Microb 2006, 72:7406-7409.
16. Karpushova A, Brummer F, Barth S, Lange S, Schmid RD. Cloning, recombinant expression and biochemical characterization of novel esterases from Bacillus sp. associated with the marine sponge Aplysina aerophoba. Appl Microl Biotechnol 2005, 67:59-69.
17. Okamura Y, Kimura T, Yokouchi H, Meneses-Osori M, Katoh M, Matsung T, Takeyama H. Isolation and charcaterization of a GDSL esterase from the metagenome of a marine sponge-associated bacteria. Mar Biotechnol 2010, 12:395-402. 10.1007/s10126-009-9226-x, 19789923.
18. Yung PY, Burke C, Lewis M, Kjelleberg S, Thomas T. Novel antibacterial proteins from the microbial communities associated with the sponge Cymbastela concentrica and the green alga Ulva australis. Appl Environ Microbiol 2011, 77:1512-1515. 10.1128/AEM.02038-10, 3067216, 21183639.
19. Hu Y, Fu C, Huang Y, Yin Y, Cheng G, Lei F, Lu N, Li J. Novel lipolytic genes from the microbial metagenomic library of the South China sea marine sediment. FEMS Microbiol Ecol 2010, 72:228-237. 10.1111/j.1574-6941.2010.00851.x, 20337707.
20. Lejon DPH, Kennedy J, Dobson ADW. Identification of novel bioactive compounds from the metagenome of the marine sponge Haliclona simulans. Handbook of Molecular Ecology II, Metagenomics in different habitats 2011, 553-562. New Jersey: Wiley-Blackwell, de Bruijn F.
21. Knox JR, Moews PC, Frere JM. Molecular evolution of bacterial β-lactam resistance. Chem Bio 1996, 3:937-947.
22. Glogauer A, Martini VP, Faoro H, Couto GH, Santos MM, Monteiro RA, Mitchell DA, Souza EM, Pedrosa FO, Krieger N. Identification and characterization of a new true lipase isolated through metagenomic approach. Microb Cell Fact 2011, 10:54. 10.1186/1475-2859-10-54, 3161859, 21762508.
23. Chu X, He H, Guo C, Sun B. Identification of two novel esterases from a marine metagenomic library derived from South China Sea. Appl Microbiol Biot 2008, 80:615-625.
24. Kim EY, Oh KH, Lee MH, Kang CH, Oh TK, Yoon JH. Novel cold-adapted alkaline lipase from an intertidal flat metagenome and proposal for a new family of bacterial lipases. Appl Environ Microb 2009, 75:257-260.
25. Fu C, Hu Y, Xie F, Guo H, Ashforth EJ, Polyak SW, Zhu B, Zhang L. Molecular cloning and characterization of a new coldactive esterase from a deep-sea metagenomic library. Appl Microbiol Biot 2011, 90:961-970.
26. Jeon JH, Lee HS, Kim JT, Kim S-J, Choi SH, Kang SG, Lee J-H. Identification of a new subfamily of salt-tolerant esterases from a metagenomic library of tidal flat sediment. Appl Microbiol Biotechnol 2011, PMID: 21720822.
27. Colak A, Pipik D, Saglam N, Guner S, Canakci S, Belduz AO. Characterization of a thermoalkalophilic esterase from a novel thermophilic bacterium, Anoxybacillus gonensis G2. Bioresour Technol 2005, 96:625-631. 10.1016/j.biortech.2004.06.003, 15501671.
28. Salameh MA, Wiegel J. Purification and characterization of two highly thermophilic alkaline lipases from Thermosyntropha lipolytica. Appl Environ Microbiol 2007, 73(23):7725-7731. 10.1128/AEM.01509-07, 10.1128/AEM.01509-07, 2168070, 17933930.
29. Voget S, Leggewie C, Uesbeck A, Raasch C, Jaeger KE, Streit WR. Prospecting for novel biocatalysts in a soil metagenome. Appl Environ Microbiol 2003, 69(10):6235-6242. 10.1128/AEM.69.10.6235-6242, 201203, 14532085.
30. Colton IJ, Ahmed SN, Kazlauskas RJ. A 2-propanol treatment increases theenantioselectivity of Candida rugosa lipase toward esters of chiral carboxylic-acids. J Org Chem 1995, 60:212-217.
31. Khuri AI, Cornell JA. Response Surfaces: Design and Analysis 1996, 1-510. New York: Marcel Dekker, 2.
32. Li W. Analysis and comparison of very large metagenomes with fast clustering and functional annotation. BMC Bioinforma 2007, 10:359.
33. Sun S, Chen J, Li W, Altinatas I, Lin A, Peltier S, Stocks K, Allen EE, Ellisman M, Grethe J, Wooley J. Community cyberinfrastructure for Advanced Microbial Ecology Research and Analysis: the CAMERA resource. Nucleic Acids Res 2011, 39(Database issue):D546-D551. 10.1093/nar/gkq1102, 3013694, 21045053.
34. Mitaku S, Hirokawa T. Physicochemical factors for discriminating between soluble and membrane proteins: hydrophobicity of helical segments and protein length. Protein Eng 1999, 12:953-957. 10.1093/protein/12.11.953, 10585500.
35. Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A. Protein Identification and Analysis Tools on the ExPASy Server. The Proteomics Protocols Handbook 2005, 571-607. Totowa: Humana Press, Walker JM.
36. Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 1987, 4:406-425.
37. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985, 39:783-791.
38. Bulow L, Mosbach K. The expression in E. coli of a polymeric gene coding for an esterase mimic catalyzing the hydrolysis of p-nitrophenyl esters. FEBS Lett 1987, 210:147-152. 10.1016/0014-5793(87)81325-X, 3025023.