Towards a cellular multi-parameter analysis platform: Fluorescence in situ hybridization (FISH) on microhole-array chips

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

Volumn , Issue , 2011, Pages 8408-8411

  Kurz, Christian M ^a   Moosdijk, Stefan V D ^a   Thielecke, Hagen ^a   Velten, Thomas ^b  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^b Department Biohybrid Systems ^*  (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYSIS METHOD; ANALYSIS SYSTEM; COST-INTENSIVE; DIFFERENTIAL PRESSURES; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN RETINA; LABOR TIME; LABVIEW SOFTWARE; MICRO HOLES; MULTIPARAMETERS; NON-INVASIVE; SEMICONDUCTOR TECHNOLOGY; STANDARD PROTOCOLS;

CELLS; COMPUTER PROGRAMMING LANGUAGES; FLUORESCENCE; FLUORESCENCE MICROSCOPY; SEMICONDUCTOR DEVICE MANUFACTURE; SILICON NITRIDE; SILICON WAFERS;

SEMICONDUCTING SILICON COMPOUNDS;

FLUORESCEIN ISOTHIOCYANATE; TEXAS RED; XANTHENE DERIVATIVE;

ARTICLE; CELL LINE; CHEMISTRY; COMPUTER PROGRAM; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; HUMAN; IMAGE PROCESSING; LIGHT; METHODOLOGY; MICROFLUIDIC ANALYSIS;

CELL LINE; FLUORESCEIN-5-ISOTHIOCYANATE; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION, FLUORESCENCE; LIGHT; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICROSCOPY, FLUORESCENCE; SOFTWARE; XANTHENES;

EID: 84861695558     PISSN: 1557170X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/IEMBS.2011.6092074     Document Type: Conference Paper

References (11)

1. K. Pachmann, J. H. Clement, C. P. Schneider, B. Willen, O. Camara, U. Pachmann and K. Hoffken, "Standardized quantification of circulating peripheral tumor cells from lung and breast cancer," Clin. Chem. Lab. Med., vol. 43, no. 6, pp. 617-627, 2005.
2. D.F.C.M. Smeets, "Historical prospective of human cytogenetics: From microscope to microarray," Clin. Biochem., vol. 37, pp. 439- 446, 2004.
3. V.J. Sieben, C.S. Debes-Marun, L.M. Pilarski and C.J. Backhouse, "An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization," Lab Chip, vol. 8, pp. 2151-2156, 2008.
4. W. King, J. Proffitt, L. Morrison, J. Piper, D. Lane and S. Seelinig, "The role of fluorescence in situ hybridization technologies in molecular diagnostics and disease management," Mol. Diagn., vol. 5, pp. 309-319, 2000.
5. V.J. Sieben, C.S. Debes Marun P.M. Pilarski, G.V. Kaigala, L.M. Pilarski and C.J. Backhouse, "Fish and chips: chromosomal analysis on microfluidic platforms," IET Nanobiotechnol., vol. 1, no. 3, pp. 27-35, 2007.
6. L. Chen, A. Manz and P.J.R. Day, "Total nucleic acid analysis integrated on microfluidic devices" Lab Chip, vol. 7, pp. 1413-1423, 2007.
7. C.M. Kurz, H. Büth, A. Sossalla, V. Vermeersch, V. Toncheva, P. Dubruel, E. Schacht, H. Thielecke, "Chip-based impedance measurement on single cells for monitoring sub-toxic effects on cell membranes," Biosens. Bioelectron., vol.26, no. 8, pp. 3405-3412, 2011.
8. U. Neugebauer, T. Bocklitz, C. Kurz, H. Thielecke, J. Clement, C. Krafft, J. Popp, Towards detection and identification of circulating tumor cells," Abstract for Spec 2010, Manchester.
9. H. Thielecke, T. Stieglitz, H. Beutel, T. Matthies, H.H. Ruf, J.U. Meyer, Fast and precise positioning of single cells on planar electrode substrates," IEEE Eng. Med. Biol., vol. 18, no. 6, pp. 48-52, 1999.
10. http://www.dako.com/cytologystep-by-step-procedure.pdf
11. C.M. Kurz, A. Maurer, K. Thees, S. Schillberg, T. Velten and H. Thielecke, Impedance-controlled cell entrapment using microholearray chips allows the isolation and selection of single, highlyproductive cells," Sensor Actuat. B-Chem., publication accepted (7 Jun 2011).