Physicochemically stable cholera toxin B subunit pentamer created by peripheral molecular constraints imposed by de novo-introduced intersubunit disulfide crosslinks

Vaccine

Volumn 30, Issue 28, 2012, Pages 4225-4232

  Miyata, Takeshi ^a   Oshiro, Satoshi ^a   Harakuni, Tetsuya ^a   Taira, Toki ^a   Matsuzaki, Goro ^a   Arakawa, Takeshi ^a  

^a UNIVERSITY OF THE RYUKYUS   (Japan)

Author keywords

AB5 enterotoxin; Adjuvant; Cholera toxin B subunit pentamer; De novo intersubunit disulfide bonds; Physicochemically stable; Vaccine

Indexed keywords

CHOLERA TOXIN B SUBUNIT; CYSTEINE; DISULFIDE; DODECYL SULFATE SODIUM; DOUBLE MUTANT CHOLERA TOXIN B SUBUNIT; GANGLIOSIDE GM1; IMMUNOGLOBULIN G; MONOSIALOGANGLIOSIDE; REDUCING AGENT; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISULFIDE BOND; FEMALE; GENE EXPRESSION; GENETIC CODE; GENETIC ENGINEERING; HEAT TREATMENT; IMMUNOGENICITY; MOUSE; MUCOSAL IMMUNITY; NONHUMAN; PHENOTYPE; PHYSICAL CHEMISTRY; PICHIA PASTORIS; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CHOLERA TOXIN; DISULFIDES; FEMALE; G(M1) GANGLIOSIDE; HYDROGEN-ION CONCENTRATION; IMMUNITY, MUCOSAL; MICE; MICE, INBRED BALB C; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; RECOMBINANT PROTEINS; TEMPERATURE;

EID: 84861656434     PISSN: 0264410X     EISSN: 18732518     Source Type: Journal    
DOI: 10.1016/j.vaccine.2012.04.047     Document Type: Article

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