A flow cytometric protocol for enumeration of endothelial progenitor cells and monocyte subsets in human blood

Journal of Immunological Methods

Volumn 381, Issue 1-2, 2012, Pages 9-13

  Hristov, Mihail ^a   Schmitz, Susanne ^a   Nauwelaers, Frans ^c   Weber, Christian ^a,b  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b Munich Heart Alliance   (Germany)

^c BD Biosciences Europe ^*  (Belgium)

Author keywords

Cellular biomarker; Endothelium; Hematopoietic stem cells; Leukocytes

Indexed keywords

CD14 ANTIGEN; CD16 ANTIGEN; FLUOROCHROME;

ADULT; ANTIGEN EXPRESSION; ARTICLE; CELL COUNT; CLINICAL PROTOCOL; CONTROLLED STUDY; ENDOTHELIAL PROGENITOR CELL; EX VIVO STUDY; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MALE; MONOCYTE; NORMAL HUMAN; PRIORITY JOURNAL;

ADULT; ANTIGENS, CD34; ANTIGENS, CD45; BLOOD CELL COUNT; ENDOTHELIAL CELLS; FEMALE; FLOW CYTOMETRY; HUMANS; MALE; MONOCYTES; REPRODUCIBILITY OF RESULTS; STEM CELLS; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2; YOUNG ADULT;

EID: 84861571739     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2012.04.003     Document Type: Article

References (19)

1. Bertolini F., Shaked Y., Mancuso P., Kerbel R.S. The multifaceted circulating endothelial cell in cancer: towards marker and target identification. Nat. Rev. Cancer 2006, 6:835.
2. Case J., Mead L.E., Bessler W.K., Prater D., White H.A., Saadatzadeh M.R., Bhavsar J.R., Yoder M.C., Haneline L.S., Ingram D.A. Human CD34+AC133+VEGFR-2+ cells are not endothelial progenitor cells but distinct, primitive hematopoietic progenitors. Exp. Hematol. 2007, 35:1109.
3. Dahmen U.M., Boettcher M., Krawczyk M., Broelsch C.E. Flow cytometric "rare event analysis": a standardized approach to the analysis of donor cell chimerism. J. Immunol. Methods 2002, 262:53.
4. Duda D.G., Cohen K.S., Scadden D.T., Jain R.K. A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Nat. Protoc. 2007, 2:805.
5. Estes M.L., Mund J.A., Mead L.E., Prater D.N., Cai S., Wang H., Pollok K.E., Murphy M.P., An C.S., Srour E.F., Ingram D.A., Case J. Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential. Cytometry A 2010, 77:831.
6. Farace F., Gross-Goupil M., Tournay E., Taylor M., Vimond N., Jacques N., Billiot F., Mauguen A., Hill C., Escudier B. Levels of circulating CD45(dim)CD34(+)VEGFR2(+) progenitor cells correlate with outcome in metastatic renal cell carcinoma patients treated with tyrosine kinase inhibitors. Br. J. Cancer 2011, 104:1144.
7. Heimbeck I., Hofer T.P., Eder C., Wright A.K., Frankenberger M., Marei A., Boghdadi G., Scherberich J., Ziegler-Heitbrock L. Standardized single-platform assay for human monocyte subpopulations: lower CD14+CD16++ monocytes in females. Cytometry A 2010, 77:823.
8. Hristov M., Weber C. Differential role of monocyte subsets in atherosclerosis. Thromb. Haemost. 2011, 106:757.
9. Hristov M., Schmitz S., Schuhmann C., Leyendecker T., von Hundelshausen P., Krötz F., Sohn H.Y., Nauwelaers F.A., Weber C. An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral blood. Cytometry A 2009, 75:848.
10. Hristov M., Leyendecker T., Schuhmann C., von Hundelshausen P., Heussen N., Kehmeier E., Krötz F., Sohn H.Y., Klauss V., Weber C. Circulating monocyte subsets and cardiovascular risk factors in coronary artery disease. Thromb. Haemost. 2010, 104:412.
11. Ingram D.A., Caplice N.M., Yoder M.C. Unresolved questions, changing definitions, and novel paradigms for defining endothelial progenitor cells. Blood 2005, 106:1525.
12. Maecker H.T., Trotter J. Flow cytometry controls, instrument setup, and the determination of positivity. Cytometry A 2006, 69A:1037.
13. Maecker H.T., Frey T., Nomura L.E., Trotter J. Selecting fluorochrome conjugates for maximum sensitivity. Cytometry A 2004, 62A:169.
14. Schmidt-Lucke C., Fichtlscherer S., Aicher A., Tschöpe C., Schultheiss H.P., Zeiher A.M., Dimmeler S. Quantification of circulating endothelial progenitor cells using the modified ISHAGE protocol. PLoS One 2010, 5:e13790.
15. Sutherland D.R., Anderson L., Keeney M., Nayar R., Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J. Hematother. 1996, 3:213.
16. Timmermans F., Van Hauwermeiren F., De Smedt M., Raedt R., Plasschaert F., De Buyzere M.L., Gillebert T.C., Plum J., Vandekerckhove B. Endothelial outgrowth cells are not derived from CD133+ cells or CD45+ hematopoietic precursors. Arterioscler. Thromb. Vasc. Biol. 2007, 27:1572.
17. Van Craenenbroeck E.M., Conraads V.M., Van Bockstaele D.R., Haine S.E., Vermeulen K., Van Tendeloo V.F., Vrints C.J., Hoymans V.Y. Quantification of circulating endothelial progenitor cells: a methodological comparison of six flow cytometric approaches. J. Immunol. Methods 2008, 332:31.
18. Werner N., Nickenig G. Influence of cardiovascular risk factors on endothelial progenitor cells: limitations for therapy?. Arterioscler. Thromb. Vasc. Biol. 2006, 26:257.
19. Ziegler-Heitbrock L., Ancuta P., Crowe S., Dalod M., Grau V., Hart D.N., Leenen P.J., Liu Y.J., MacPherson G., Randolph G.J., Scherberich J., Schmitz J., Shortman K., Sozzani S., Strobl H., Zembala M., Austyn J.M., Lutz M.B. Nomenclature of monocytes and dendritic cells in blood. Blood 2010, 116:e74.