Detection of heartworm infection in dogs via PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples

American Journal of Veterinary Research

Volumn 73, Issue 6, 2012, Pages 854-859

  Crowder Dr , Christopher D ^a   Matthews, Heather E ^a   Rounds, Megan A ^a   Li, Feng ^a   Schutzer, Steven E ^b   Sampath, Ranga ^a   Hofstadler, Steven A ^a   Ecker, David J ^a   Eshoo, Mark W ^a  

^a Ibis Biosciences Inc   (United States)

^b RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEIC ACID; PARASITE ANTIGEN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DIROFILARIA IMMITIS; DIROFILARIASIS; DOG DISEASE; ELECTROSPRAY MASS SPECTROMETRY; ENDOSYMBIONT; FEMALE; MALE; MICROFILARIA (NEMATODE LARVA); NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; WOLBACHIA;

ANIMALS; BASE SEQUENCE; DIROFILARIASIS; DNA PRIMERS; DNA, RIBOSOMAL; DOG DISEASES; DOGS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

CANIS FAMILIARIS; DIROFILARIA IMMITIS; WOLBACHIA;

EID: 84861548031     PISSN: 00029645     EISSN: None     Source Type: Journal    
DOI: 10.2460/ajvr.73.6.854     Document Type: Article

References (19)

1. Mendoza N, Li A, Gill A, et al. Filariasis: diagnosis and treatment. Dermatol Ther 2009;22:475-490.
2. Genchi C, Rinaldi L, Mortarino M, et al. Climate and Dirofilaria infection in Europe. Vet Parasitol 2009;163:286-292.
3. Simon F, Morchon R, Gonzalez-Miguel J, et al. What is new about animal and human dirofilariosis? Trends Parasitol 2009;25:404-409.
4. Sironi M, Bandi C, Sacchi L, et al. Molecular evidence for a close relative of the arthropod endosymbiont Wolbachia in a filarial worm. Mol Biochem Parasitol 1995;74:223-227.
5. Bandi C, McCall JW, Genchi C, et al. Effects of tetracycline on the filarial worms Brugia pahangi and Dirofilaria immitis and their bacterial endosymbionts Wolbachia. Int J Parasitol 1999;29:357-364.
6. Taylor MJ, Bandi C, Hoerauf A. Wolbachia bacterial endosymbionts of filarial nematodes. Adv Parasitol 2005;60:245-284.
7. Kramer LH, Tamarozzi F, Morchon R, et al. Immune response to and tissue localization of the Wolbachia surface protein (WSP) in dogs with natural heartworm (Dirofilaria immitis) infection. Vet Immunol Immunopathol 2005;106:303-308.
8. Kramer L, Simon F, Tamarozzi F, et al. Is Wolbachia complicating the pathological effects of Dirofilaria immitis infections? Vet Parasitol 2005;133:133-136.
9. McCall JW, Genchi C, Kramer L, et al. Heartworm and Wolbachia: therapeutic implications. Vet Parasitol 2008;158:204-214.
10. Crowder CD, Matthews HE, Schutzer S, et al. Genotypic variation and mixtures of Lyme Borrelia in Ixodes ticks from North America and Europe. PLoS ONE 2010;5:e10650.
11. Eshoo MW, Crowder CD, Li H, et al. Detection and identification of Ehrlichia species in blood by use of PCR and electrospray ionization mass spectrometry. J Clin Microbiol 2010;48:472-478.
12. Grant-Klein RJ, Baldwin CD, Turell MJ, et al. Rapid identification of vector-borne flaviviruses by mass spectrometry. Mol Cell Probes 2010;24:219-228.
13. Crowder CD, Rounds MA, Phillipson CA, et al. Extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens. J Med Entomol 2010;47:89-94.
14. Eshoo MW, Whitehouse CA, Zoll ST, et al. Direct broadrange detection of alphaviruses in mosquito extracts. Virology 2007;368:286-295.
15. Ecker JA, Massire C, Hall TA, et al. Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry. J Clin Microbiol 2006;44:2921-2932.
16. Muddiman DC, Anderson GA, Hofstadler SA, et al. Length and base composition of PCR-amplified nucleic acids using mass measurements from electrospray ionization mass spectrometry. Anal Chem 1997;69:1543-1549.
17. Duncan AW, Correa MT, Levine JF, et al. The dog as a sentinel for human infection: prevalence of Borrelia burgdorferi C6 antibodies in dogs from southeastern and mid-Atlantic states. Vector Borne Zoonotic Dis 2005;5:101-109.
18. Hamer SA, Tsao JI, Walker ED, et al. Use of tick surveys and serosurveys to evaluate pet dogs as a sentinel species for emerging Lyme disease. Am J Vet Res 2009;70:49-56.
19. Nelson CT, McCall JW, Rubin SB, et al. 2005 guidelines for the diagnosis, prevention and management of heartworm (Dirofilaria immitis) infection in dogs. Vet Parasitol 2005;133:255-266.