Extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens

Journal of Medical Entomology

Volumn 47, Issue 1, 2010, Pages 89-94

  Crowder, Chris D ^a   Rounds, Megan A ^a   Phillipson, Curtis A ^a   Picuri, John M ^a   Matthews, Heather E ^a   Halverson, Justina ^a   Schutzer, Steven E ^a,b   Ecker, David J ^a   Eshoo, Mark W ^a  

^a Ibis Biosciences Inc   (United States)

^b RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

Borrelia; Nucleic acid extraction; Powassan virus; Tick; Vector borne disease

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; DNA; PRIMER DNA; PROTOZOAL DNA; PROTOZOAL RNA; RNA; VIRUS DNA; VIRUS RNA;

ANIMAL; ARTICLE; BORRELIA; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TICK; TICK BORNE DISEASE; TICK BORNE ENCEPHALITIS FLAVIVIRUS; VIROLOGY;

ANIMALS; BORRELIA; DNA; DNA PRIMERS; DNA, BACTERIAL; DNA, PROTOZOAN; DNA, VIRAL; ENCEPHALITIS VIRUSES, TICK-BORNE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, BACTERIAL; RNA, PROTOZOAN; RNA, VIRAL; TICK-BORNE DISEASES; TICKS;

ANIMALIA; ARTHROPODA; BACTERIA (MICROORGANISMS); BORRELIA; BORRELIA BURGDORFERI; IXODES SCAPULARIS; IXODIDA; POWASSAN VIRUS; PROTOZOA; SIPHONAPTERA (FLEAS); TICK-BORNE ENCEPHALITIS VIRUS;

EID: 75149150918     PISSN: 00222585     EISSN: None     Source Type: Journal    
DOI: 10.1603/033.047.0112     Document Type: Article

References (25)

1. Allender, C. J., W. R. Easterday, M. N. Van Ert, D. M. Wagner, and P. Keim. 2004. High-throughput extraction of arthropod vector and pathogen DNA using bead milling. Biotechniques 37: 730. 732: 734.
2. Baldwin, C. D., G. B. Howe, R. Sampath, L. B. Blyn, H. Matthews, V. Harpin, T. A. Hall, J. J. Drader, S. A. Hofstadler, M. W. Eshoo, et al. 2009. Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates. Diagn. Microbiol. Infect. Dis. 63: 403-408.
3. Beaglehole, R., A. Irwin, and T. Prentice. 2004. World Health Report 2004: changing history. World Health Organization, Geneva, Switzerland.
4. Bock, R., L. Jackson, A. de Vos, and W. Jorgensen. 2004. Babesiosis of cattle. Parasitology 129(suppl): S247-S269.
5. Clay, K., O. Klyachko, N. Grindle, D. Civitello, D. Oleske, and C. Fuqua. 2008. Microbial communities and interactions in the lone star tick, Amblyomma americanum. Mol Ecol. 73: 4374-4381.
6. de la Fuente, J., A. Estrada-Pena, J. M. Venzal, K. M. Kocan, and D. E. Sonenshine. 2008. Overview: ticks as vectors of pathogens that cause disease in humans and animals. Front. Biosci. 13: 6938-6946.
7. Ecker, D. J., R. Sampath, C. Massire, L. B. Blyn, T. A. Hall, M. W. Eshoo, and S. A. Hofstadler. 2008. Ibis T5000: a universal biosensor approach for microbiology. Nat. Rev. Microbiol. 6: 553-558.
8. Ecker, D. J., R. Sampath, L. B. Blyn, M. W. Eshoo, C. Ivy, J. A. Ecker, B. Libby, V. Samant, K. A. Sannes-Lowery, R. E. Melton, et al. 2005. Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance. Proc. Natl. Acad. Sci. U.S.A. 102: 8012-8017.
9. Ecker, J. A., C. Massire, T. A. Hall, R. Ranken, T. T. Pennella, C. Agasino Ivy, L. B. Blyn, S. A. Hofstadler, T. P. Endy, P. T. Scott, et al. 2006. Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry. J. Clin. Microbiol. 44: 2921-2932.
10. Ergonul, O. 2006. Crimean-Congo haemorrhagic fever. Lancet. Infect. Dis. 6: 203-214.
11. Eshoo, M. W., C. A. Whitehouse, A. Nalca, S. Zoll, J. A. Ecker, T. A. Hall, T. T. Pennella, D. D. Duncan, A. Desai, E. K. Moradi, et al. 2009. Rapid and high-throughput pan-Orthopoxvirus detection and identification using PCR and mass spectrometry. PLoS. ONE. 4: e6342.
12. Eshoo, M. W., C. A. Whitehouse, S. T. Zoll, C. Massire, T. T. Pennella, L. B. Blyn, R. Sampath, T. A. Hall, J. A. Ecker, A. Desai, et al. 2007. Direct broad-range detection of alphaviruses in mosquito extracts. Virology 368: 286-295.
13. Exner, M. M., and M. A. Lewinski. 2003. Isolation and detection of Borrelia burgdorferi DNA from cerebral spinal fluid, synovial fluid, blood, urine, and ticks using the Roche MagNA Pure system and real-time PCR. Diagn. Microbiol. Infect. Dis. 46: 235-240.
14. Halos, L., T. Jamal, L. Vial, R. Maillard, A. Suau, A. Le Menach, H. J. Boulouis, and M. Vayssier-Taussat. 2004. Determination of an efficient and reliable method for DNA extraction from ticks. Vet. Res. 35: 709-713.
15. Hill, C. A., and J. A. Gutierrez. 2003. A method for extraction and analysis of high quality genomic DNA from ixodid ticks. Med. Vet. Entomol. 17: 224-227.
16. Kalluri, S., P. Gilruth, D. Rogers, and M. Szczur. 2007. Surveillance of arthropod vector-borne infectious diseases using remote sensing techniques: a review. PLoS. Pathog. 3: 1361-1371.
17. Lindquist, L., and O. Vapalahti. 2008. Tick-borne encephalitis. Lancet 371: 1861-1871.
18. McNabb, S., and R. A. Jajosky. 2008. Summary of notifiable diseases-United States, 2006, morbidity and mortality weekly report. Centers for Disease Control and Prevention, Atlanta, GA.
19. Moriarity, J. R., A. D. Loftis, and G. A. Dasch. 2005. Highthroughput molecular testing of ticks using a liquid-handling robot. J. Med. Entomol. 42: 1063-1067.
20. Muddiman, D. C., G. A. Anderson, S. A. Hofstadler, and R. D. Smith. 1997. Length and base composition of PCR-amplified nucleic acids using mass measurements from electrospray ionization mass spectrometry. Anal. Chem. 69: 1543-1549.
21. O'Connor, K. A., and D. R. Zusman. 1988. Reexamination of the role of autolysis in the development of Myxococcus xanthus. J. Bacteriol. 170: 4103-4112.
22. Romero, J. R., and K. A. Simonsen. 2008. Powassan encephalitis and Colorado tick fever. Infect. Dis. Clin. North.Am. 22: 545-559, x.
23. Sampath, R., K. L. Russell, C. Massire, M. W. Eshoo, V. Harpin, L. B. Blyn, R. Melton, C. Ivy, T. Pennella, F. Li, et al. 2007. Global surveillance of emerging Influenza virus genotypes by mass spectrometry. PLoS. ONE. 2: e489.
24. Steiner, F. E., R. R. Pinger, C. N. Vann, N. Grindle, D. Civitello, K. Clay, and C. Fuqua. 2008. Infection and co-infection rates of Anaplasma phagocytophilum variants, Babesia spp., Borrelia burgdorferi, and the rickettsial endosymbiont in Ixodes scapularis (Acari: Ixodidae) from sites in Indiana, Maine, Pennsylvania, and Wisconsin. J. Med. Entomol. 45: 289-297.
25. Vannier, E., B. E. Gewurz, and P. J. Krause. 2008. Human babesiosis. Infect. Dis. Clin. North. Am. 22: 469-488, viii-ix.