Dexamethasone suppression test: Development of a method for simultaneous determination of cortisol and dexamethasone in human plasma by liquid chromatography/tandem mass spectrometry

Annals of Clinical Biochemistry

Volumn 49, Issue 2, 2012, Pages 170-176

  Hempen, C ^a   Elfering, S ^a   Mulder, A H L ^b   Van Den Bergh, F A J T M ^a   Maatman, R G H J ^b  

^a MEDISCH SPECTRUM TWENTE   (Netherlands)

^b Hospital Group Twente Laboratory   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DEXAMETHASONE; HYDROCORTISONE;

ACCURACY; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DEXAMETHASONE SUPPRESSION TEST; DRUG DETERMINATION; HUMAN; HUMAN TISSUE; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; METHODOLOGY; PLASMA; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY;

CHROMATOGRAPHY, LIQUID; DEXAMETHASONE; HUMANS; HYDROCORTISONE; LIMIT OF DETECTION; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EID: 84861487342     PISSN: 00045632     EISSN: 17581001     Source Type: Journal    
DOI: 10.1258/acb.2011.011004     Document Type: Article

References (13)

1. Schleimer RP. An overview of glucocorticoid anti-inflammatory actions. Eur J Clin Pharmacol 1993;45(Suppl. 1):S3-7
2. De Keyser J, Zwanikken C, Zorgdrager A, Oenema D, Boon M. Treatment of acute relapses in multiple sclerosis at home dexamethasone: a pilot study. J Clin Neurosci 1999;6:382-4
3. Greenspan FS. Basic and Clinical Endocrinology. 3rd edn. USA: Prentice-Hall Int Inc., 1991
4. Nieman LK, Biller BMK, Findling JW, et al. The diagnosis of Cushing's syndrome: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab 2008;93:1526-40
5. Vilar L, Da Conceicao Freitas M, Faria M, et al. Pitfalls in the diagnosis of Cushing's syndrome. Arq Bras Endorcinol Metab 2007;51:1207-16
6. Meikle AW. Dexamethasone suppression tests: usefulness of simultaneous measurement of plasma cortisol and dexamethasone. Clin Endocrinol 1982;16:401-8
7. Morris H, Varr V, Gilliland J, Hooper M. Dexamethasone concentrations and the dexamethasone suppression test in psychiatric disorders. Br J Psychiatry 1986;148:66-9
8. O'Sullivan BT, Cutler DJ, Hunt GE, Walters C, Johnson GF, Caterson ID. Pharmacokinetics of dexamethasone and its relationship to dexamethasone suppression test outcome in depressed patients and healthy control subjects. Biol Psychiatry 1997;41:574-84
9. Chen YL, Jiang X, Weng N. A liquid chromatographic-tandem mass spectrometric method for the quantitative analysis of dexamethasone in human plasma. J Liq Chromatogr Relat Tech 2002;25:1317-34
10. Frerichs VA, Toernatore KM. Determination of the glucocorticoids prednisone, prednisolone, dexamethasone, and cortisol in human serum using liquid chromatography coupled to tandem mass spectrometry. J Chromatogr B 2004;802:329-38
11. Pearson Murphy PE. Steroids and depression. J Steroid Biochem Mol Biol 1991;38:537-59
12. Viswanathan CT, Bansal S, Booth B, et al. Quantitative bioanalytical methods validation and implementation: best practices for chromatographic and ligand binding assays. Pharm Res 2007;24:1962-1973
13. Taylor RL, Grebe SK, Singh RJ. Quantitative, highly sensitive liquid chromatography-tandem mass spectrometry method for detection of synthetic corticosteroids. Clin Chem 2004;50:2345-52