Screening of anti-cancer agent using zebrafish: Comparison with the MTT assay

Biochemical and Biophysical Research Communications

Volumn 422, Issue 1, 2012, Pages 85-90

  Li, Yigen ^a   Huang, Wenjin ^b   Huang, Shenyuan ^a   Du, Jiulin ^b   Huang, Cheng ^a  

^a SHANGHAI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

Anti cancer drug; Apoptosis; MTT assay; P53; Zebrafish

Indexed keywords

ALPHA CHACONINE; AMPHOTERICIN B; ANTIMYCIN A1; ANTINEOPLASTIC AGENT; AUSTRALINE; BETA LAPACHONE; BREFELDIN A; BUFALIN; CAMPTOTHECIN; CAPSAICIN; CHELIDONINE; CINOBUFAGIN; CYCLOHEXIMIDE; CYTOCHALASIN E; DAIDZEIN; DEGUELIN; ECHINOMYCIN; EPIBATIDINE; GLIOTOXIN; HARMINE; HYPOCRELLIN A; IVERMECTIN; MENADIONE; NARASIN; PLUMBAGIN; PODOPHYLLOTOXIN; ROTENONE; SANGUINARINE; UNINDEXED DRUG; VINPOCETINE;

ANIMAL CELL; APOPTOSIS; ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CONTROLLED STUDY; DRUG POTENCY; DRUG SCREENING; EMBRYO; EMBRYO DEVELOPMENT; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; ZEBRA FISH;

ANIMALS; ANTINEOPLASTIC AGENTS; APOPTOSIS; BIPHENYL COMPOUNDS; CAMPTOTHECIN; CELL LINE, TUMOR; COLORING AGENTS; DIMETHYL SULFOXIDE; DRUG SCREENING ASSAYS, ANTITUMOR; EMODIN; GENE EXPRESSION; HUMANS; LIGNANS; NAPHTHOQUINONES; ROTENONE; TETRAZOLIUM SALTS; THIAZOLES; TRANSCRIPTIONAL ACTIVATION; TUMOR SUPPRESSOR PROTEIN P53; ZEBRAFISH;

DANIO RERIO;

EID: 84861461646     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.04.110     Document Type: Article

References (29)

1. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65:55-63.
2. Cory A.H., Owen T.C., Barltrop J.A., Cory J.G. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun. 1991, 3:207-212.
3. Riss T.L., Moravec R.A. Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Dev. Technol. 2004, 2:51-62.
4. Bowman T.V., Zon L.I. Swimming into the future of drug discovery: in vivo chemical screens in zebrafish. ACS Chem. Biol. 2010, 5:159-161.
5. Huang H.T., Zon L.I. Regulation of stem cells in the zebra fish hematopoietic system. Cold Spring Harb. Symp. Quant. Biol. 2008, 73:111-118.
6. Ingham P.W. The power of the zebrafish for disease analysis. Hum. Mol. Genet. 2009, 18:R107-R112.
7. Amatruda J.F., Patton E.E. Genetic models of cancer in zebrafish. Int. Rev. Cell. Mol. Biol. 2008, 271:1-34.
8. Chico T.J., Ingham P.W., Crossman D.C. Modeling cardiovascular disease in the zebrafish. Trends Cardiovasc. Med. 2008, 18:150-155.
9. Liu S., Leach S.D. Zebrafish models for cancer. Annu. Rev. Pathol. 2011, 6:71-93.
10. Parng C., Seng W.L., Semino C., McGrath P. Zebrafish: a preclinical model for drug screening. Assay Drug Dev. Technol. 2002, 1:41-48.
11. Hao J., Williams C.H., Webb M.E., Hong C.C. Large scale zebrafish-based in vivo small molecule screen. J. Vis. Exp. 2010, 46:e2243. 10.3791/2243.
12. Wang Y.H., Cheng C.C., Lee W.J., Chiou M.L., Pai C.W., Wen C.C., Chen W.L., Chen Y.H. A novel phenotype-based approach for systematically screening antiproliferation metallodrugs. Chem. Biol. Interact. 2009, 182:84-91.
13. Crawford A.D., Esguerra C.V., de Witte P.A. Fishing for drugs from nature: zebrafish as a technology platform for natural product discovery. Planta Med. 2008, 74:624-632.
14. Chakraborty C., Hsu C.H., Wen Z.H., Lin C.S., Agoramoorthy G. Zebrafish: a complete animal model for in vivo drug discovery and development. Curr. Drug Metab. 2009, 10:116-124.
15. Kari G., Rodeck U., Dicker A.P. Zebrafish: an emerging model system for human disease and drug discovery. Clin. Pharmacol. Ther. 2007, 82:70-80.
16. Stern H.M., Murphey R.D., Shepard J.L., Amatruda J.F., Straub C.T., Pfaff K.L., Weber G., Tallarico J.A., King R.W., Zon L.I. Small molecules that delay S phase suppress a zebrafish bmyb mutant. Nat. Chem. Biol. 2005, 1:366-370.
17. Lally B.E., Geiger G.A., Kridel S., Arcury-Quandt A.E., Robbins M.E., Kock N.D., Wheeler K., Peddi P., Georgakilas A., Kao G.D., Koumenis C. Identification and biological evaluation of a novel and potent small molecule radiation sensitizer via an unbiased screen of a chemical library. Cancer Res. 2007, 67:8791-8799.
18. Eimon P.M., Ashkenazi A. The zebrafish as a model organism for the study of apoptosis. Apoptosis 2010, 15:331-349.
19. Huang C., Gu S., Yu P., Yu F., Feng C., Gao N., Du J. Deficiency of smarcal1 causes cell cycle arrest and developmental abnormalities in zebrafish. Dev. Biol. 2010, 339:89-100.
20. Tsonis P.A. Embryogenesis and carcinogenesis: order and disorder. Anticancer Res. 1987, 7:617-623.
21. Gostjeva E.V., Zukerberg L., Chung D., Thilly W.G. Bell-shaped nuclei dividing by symmetrical and asymmetrical nuclear fission have qualities of stem cells in human colonic embryogenesis and carcinogenesis. Cancer Genet. Cytogenet. 2006, 164:16-24.
22. Darzynkiewicz Z., Bruno S., Del Bino G., Traganos F. The cell cycle effects of camptothecin. Ann. NY Acad. Sci. 1996, 803:93-100.
23. Pizarro J.G., Folch J., Junyent F., Verdaguer E., Auladell C., Beas-Zarate C., Pallas M., Camins A. Antiapoptotic effects of roscovitine on camptothecin-induced DNA damage in neuroblastoma cells. Apoptosis 2011, 16:536-550.
24. Wuerzberger S.M., Pink J.J., Planchon S.M., Byers K.L., Bornmann W.G., Boothman D.A. Induction of apoptosis in MCF-7: WS8 breast cancer cells by beta-lapachone. Cancer Res. 1998, 58:1876-1885.
25. Moon H.S., Jacobson E.M., Khersonsky S.M., Luzung M.R., Walsh D.P., Xiong W., Lee J.W., Parikh P.B., Lam J.C., Kang T.W., Rosania G.R., Schier A.F., Chang Y.T. A novel microtubule destabilizing entity from orthogonal synthesis of triazine library and zebrafish embryo screening. J. Am. Chem. Soc. 2002, 124:11608-11609.
26. Ewan K., Pajak B., Stubbs M., Todd H., Barbeau O., Quevedo C., Botfield H., Young R., Ruddle R., Samuel L., Battersby A., Raynaud F., Allen N., Wilson S., Latinkic B., Workman P., McDonald E., Blagg J., Aherne W., Dale T. A useful approach to identify novel small-molecule inhibitors of Wnt-dependent transcription. Cancer Res. 2010, 70:5963-5973.
27. Don M.J., Chang Y.H., Chen K.K., Ho L.K., Chau Y.P. Induction of CDK inhibitors (p21(WAF1) and p27(Kip1)) and Bak in the beta-lapachone-induced apoptosis of human prostate cancer cells. Mol. Pharmacol. 2001, 59:784-794.
28. Langheinrich U., Hennen E., Stott G., Vacun G. Zebrafish as a model organism for the identification and characterization of drugs and genes affecting p53 signaling. Curr. Biol. 2002, 12:2023-2028.
29. Berghmans S., Murphey R.D., Wienholds E., Neuberg D., Kutok J.L., Fletcher C.D., Morris J.P., Liu T.X., Schulte-Merker S., Kanki J.P., Plasterk R., Zon L.I., Look A.T. Tp53 mutant zebrafish develop malignant peripheral nerve sheath tumors. Proc. Natl. Acad. Sci. USA 2005, 102:407-412.