Quantification of kinase activity in cell lysates via photopatterned macroporous poly(ethylene glycol) hydrogel arrays in microfluidic channels

Biomedical Microdevices

Volumn 14, Issue 2, 2012, Pages 247-257

  Lee, Andrew G ^a   Beebe, David J ^b   Palecek, Sean P ^a,b  

^a Wisconsin Electric Machines and Power Electronics Consortium   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Cancer diagnostic; Hydrogels; Kinase activity; Microfluidic device

Indexed keywords

BIOCHEMICAL RESPONSE; CANCER CELLS; CANCER DEVELOPMENT; CANCER DIAGNOSTICS; CANCER THERAPEUTICS; CELL LYSATES; CELLULAR LYSATES; FUNCTIONALIZED; IMATINIB MESYLATE; K562 CELLS; KINASE ACTIVITY; KINASE INHIBITORS; KINASE SUBSTRATES; MACRO-POROSITY; MACROPOROUS; MACROPOROUS HYDROGELS; MICRO-FLUIDIC DEVICES; MICROFLUIDIC CHANNEL; MINIMALLY INVASIVE; MOLECULAR REGULATION; PHOTOPATTERNED; POLY(ETHYLENE GLYCOL) HYDROGEL; POLYETHYLENE GLYCOL DIACRYLATE; POROGENS; SAMPLE VOLUME; SENSITIVE ASSAY; STANDARD PROTEINS; TISSUE SAMPLES; TYROSINE KINASE; TYROSINE KINASE INHIBITOR;

AMINO ACIDS; ASSAYS; CELLS; CYTOLOGY; DISEASES; ENZYMES; FLUIDIC DEVICES; MICROFLUIDICS; PATIENT TREATMENT; POLYETHYLENE GLYCOLS; TISSUE;

HYDROGELS;

ACRYLIC ACID DERIVATIVE; BCR ABL PROTEIN; GST CRK1 FUSION PROTEIN; HYBRID PROTEIN; IMATINIB; POLY(ETHYLENE GLYCOL) DIACRYLATE; UNCLASSIFIED DRUG;

ARTICLE; CELL LYSATE; CELL STRAIN K 562; CHEMICAL PROCEDURES; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFICACY; DRUG RESPONSE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME IMMOBILIZATION; ENZYME INHIBITION; ENZYME SUBSTRATE; HYDROGEL; IC 50; MACROPOROSITY; MICROFLUIDIC CHANNEL; MICROFLUIDICS; PHOTOPATTERNING; POROSITY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REACTION TIME; SENSITIVITY AND SPECIFICITY;

FUSION PROTEINS, BCR-ABL; HUMANS; HYDROGELS; INHIBITORY CONCENTRATION 50; K562 CELLS; MICROFLUIDICS; PHOSPHORYLATION; PIPERAZINES; POLYETHYLENE GLYCOLS; PROTEIN KINASE INHIBITORS; PROTEIN-TYROSINE KINASES; PYRIMIDINES;

EID: 84861459941     PISSN: 13872176     EISSN: 15728781     Source Type: Journal    
DOI: 10.1007/s10544-011-9602-y     Document Type: Article

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