Low copy number DNA profiling from isolated sperm using the aureka®-micromanipulation system

Forensic Science International: Genetics

Volumn 6, Issue 4, 2012, Pages 461-465

  Schneider, C ^a   Muller U ^b   Kilper, R ^b   Siebertz, B ^a  

^a Galantos Genetics GmbH   (Germany)

^b Aura Optik GmbH   (Germany)

Author keywords

Aureka cell isolation; HY LITER specific sperm staining; Low copy number PCR

Indexed keywords

MICROSPHERE;

ARTICLE; CELL ISOLATION; CONTROLLED STUDY; DNA FINGERPRINTING; FEMALE; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; FORENSIC GENETICS; HUMAN; HUMAN CELL; LOW COPY NUMBER DNA PROFILING; MALE; MICROMANIPULATION; MICROMANIPULATOR; NORMAL HUMAN; PRIORITY JOURNAL; SEMEN ANALYSIS; SHORT TANDEM REPEAT; STAINING;

CELL SEPARATION; DNA; DNA FINGERPRINTING; ELECTROPHORESIS, CAPILLARY; EPITHELIAL CELLS; FEMALE; HUMANS; MALE; MICROSATELLITE REPEATS; MICROSCOPY, FLUORESCENCE; POLYMERASE CHAIN REACTION; SPERMATOZOA; STAINING AND LABELING;

EID: 84861331432     PISSN: 18724973     EISSN: 18780326     Source Type: Journal    
DOI: 10.1016/j.fsigen.2011.10.001     Document Type: Article

References (14)

1. P. Gill, A.J. Jeffreys, and D.J. Werrett Forensic application of DNA fingerprints Nature 318 1985 577 579
2. K. Anslinger, B. Mack, B. Bayer, B. Rolf, and W. Eisenmenger Digoxigenin labelling and laser capture microdissection of male cells Int. J. Legal Med. 119 2005 374 377
3. K. Anslinger, B. Bayer, B. Mack, and W. Eisenmenger Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling Int. J. Legal Med. 121 2007 54 56
4. M. Vandewoestyne, D. Hoofstat, F. van Nieuwerburgh, and D. Deforce Automatic detection of spermatozoa for laser capture microdissection Int. J. Legal Med. 123 2009 169 175
5. C. Sanders, E.K. Reisenbigler, D. Peterson, Laser microdissection separation of pure spermatozoa populations from mixed cell samples for forensic DNA analysis, Award Number: 2004-DN-BX-K215 U.S. Department of Justice, 2007.
6. C. Petit, V. Martel-Petit, R. Hienne, and S. Frackowiak Single sperm cell isolation by micromanipulation for human identification in sexual assault Forensic Sci. Int. Genet. (Supplement Series) 1 2008 60 62
7. J. Old, M. Fahrner, J. Wu, C.G. Westring, P.W. Boonlayangoor, and K. Reich Summary results of a blinded study on the effectiveness and efficiency of using SPERM HY-LITER™ to screen sexual assault evidence for sperm 20th International Symposium on Human Identification 2009
8. S. Brück, H. Evers, F. Heidorn, U. Müller, R. Kilper, and M.A. Verhoff Single cells for forensic DNA analysis - from evidence material to test tube J. Forensic Sci. 56 2011 176 180
9. R. Kilper, Apparatus and method for picking up, transporting and depositing microscopic samples, WO2008037305 (2008).
10. C.C.G. Benschop, C.P. van der Beek, H.C. Meiland, A.G.M. van Gorp, A.A. Westen, and T. Sijen Low template STR typing: effect of replicate number and consensus method on genotyping reliability and DNA database search results Forensic Sci. Int. 5 2011 316 328
11. D. Di Martino, G. Giuffre, N. Staiti, A. Simone, M. Le Donne, and L. Saravo Single sperm cell isolation by laser microdissection Forensic Sci. Int. 146S 2004 151 153
12. T. Murase, H. Inagaki, and T. Eimoto Influence of histochemical and immunohistochemical stains on polymerase chain reaction Mod. Pathol. 13 2 2000 147 151
13. J.A. Nicklas, and E. Buel Development of an Alu-based, real-time PCR method for quantitation of human DNA in forensic samples J. Forensic Sci. 48 5 2003 936 944
14. L. Caixia, Q. Bing, J. Anquan, X. Xiulan, and H. Lan The combination of single cell micromanipulation with LV-PCR system and its application in forensic science Forensic Sci. Int. Genet. (Supplement Series) 2 2009 516 517