Role of conserved cysteine residues in hepatitis C virus glycoprotein E2 folding and function

Journal of Virology

Volumn 86, Issue 7, 2012, Pages 3961-3974

  McCaffrey, Kathleen ^a,b   Boo, Irene ^a   Tewierek, Kevin ^a   Edmunds, Mark L ^a   Poumbourios, Pantelis ^a,c   Drummer, Heidi E ^a,b,c  

^a BURNET INSTITUTE   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; CYSTEINE; DISULFIDE; GLYCOPROTEIN E2;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DIMERIZATION; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; MUTAGENESIS; MUTANT; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEPLETION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STRUCTURE; PSEUDOTYPING; RETROVIRUS; VIRION; VIRUS ENTRY; WILD TYPE;

AMINO ACID SEQUENCE; CELL LINE; CONSERVED SEQUENCE; CYSTEINE; HEPACIVIRUS; HEPATITIS C; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN FOLDING; VIRAL ENVELOPE PROTEINS; VIRUS INTERNALIZATION;

HEPATITIS C VIRUS;

EID: 84861313031     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.05396-11     Document Type: Article

References (35)

1. Bartosch B, Dubuisson J, Cosset FL. 2003. Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes. J. Exp. Med. 197:633- 642.
2. Benedicto I, et al. 2009. The tight junction-associated protein occludin is required for a postbinding step in hepatitis C virus entry and infection. J. Virol. 83:8012- 8020.
3. Brazzoli M, et al. 2005. Folding and dimerization of hepatitis C virus E1 and E2 glycoproteins in stably transfectedCHOcells. Virology 332:438-453.
4. Bressanelli S, et al. 2004. Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation.EMBOJ. 23:728-738.
5. Choukhi A, Ung S, Wychowski C, Dubuisson J. 1998. Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins. J. Virol. 72:3851-3858.
6. Ciczora Y, et al. 2005. Contribution of the charged residues of hepatitis C virus glycoprotein E2 transmembrane domain to the functions of the E1E2 heterodimer. J. Gen. Virol. 86:2793-2798.
7. Ciczora Y, Callens N, Penin F, Pecheur EI, Dubuisson J. 2007. Transmembrane domains of hepatitis C virus envelope glycoproteins: residues involved in E1E2 heterodimerization and involvement of these domains in virus entry. J. Virol. 81:2372-2381.
8. Drummer HE, Boo I, Maerz AL, Poumbourios P. 2006. A conserved Gly436-Trp-Leu-Ala-Gly-Leu-Phe-Tyr motif in hepatitis C virus glycoprotein E2 is a determinant of CD81 binding and viral entry. J. Virol. 80:7844 -7853.
9. Drummer HE, Maerz A, Poumbourios P. 2003. Cell surface expression of functional hepatitisCvirus E1 and E2 glycoproteins. FEBS Lett. 546:385-390.
10. Drummer HE, Poumbourios P. 2004. Hepatitis C virus glycoprotein E2 contains a membrane-proximal heptad repeat sequence that is essential for E1E2 glycoprotein heterodimerization and viral entry. J. Biol. Chem. 279:30066 -30072.
11. Drummer HE, Wilson KA, Poumbourios P. 2002. Identification of the hepatitis C virus e2 glycoprotein binding site on the large extracellular loop of CD81. J. Virol. 76:11143-11147.
12. Evans MJ, et al. 2007. Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry. Nature 446:801- 805.
13. Fraser J, Boo I, Poumbourios P, Drummer HE. 2011. Hepatitis C virus (HCV) envelope glycoproteins e1 and e2 contain reduced cysteine residues essential for virus entry. J. Biol. Chem. 286:31984 -31992.
14. Harris HJ, et al. 2010. Claudin association with CD81 defines hepatitis C virus entry. J. Biol. Chem. 285:21092-21102.
15. Harris HJ, et al. 2008. CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry. J. Virol. 82:5007-5020.
16. Kato N, et al. 1992. Characterization of hypervariable regions in the putative envelope protein of hepatitis C virus. Biochem. Biophys. Res. Commun. 189:119 -127.
17. Keck ZY, et al. 2004. Hepatitis C virus e2 has three immunogenic domains containing conformational epitopes with distinct properties and biological functions. J. Virol. 78:9224 -9232.
18. Kitadokoro K, et al. 2001. CD81 extracellular domain 3D structure: insight into the tetraspanin superfamily structural motifs.EMBOJ. 20:12-18.
19. Krey T, et al. 2010. The disulfide bonds in glycoprotein E2 of hepatitis C virus reveal the tertiary organization of the molecule. PLoS Pathog. 6:e1000762.
20. Marukian S, et al. 2008. Cell culture-produced hepatitis C virus does not infect peripheral blood mononuclear cells. Hepatology 48:1843-1850.
21. McCaffrey K, Boo I, Poumbourios P, Drummer HE. 2007. Expression and characterization of a minimal hepatitis C virus glycoprotein E2 core domain that retains CD81 binding. J. Virol. 81:9584 -9590.
22. Meertens L, et al. 2008. The tight junction proteins claudin-1, -6, and -9 are entry cofactors for hepatitis C virus. J. Virol. 82:3555-3560.
23. Michalak JP, et al. 1997. Characterization of truncated forms of hepatitis C virus glycoproteins. J. Gen. Virol. 78:2299 -2306.
24. Owsianka AM, et al. 2006. Identification of conserved residues in the E2 envelope glycoprotein of the hepatitis C virus that are critical for CD81 binding. J. Virol. 80:8695- 8704.
25. Pileri P, et al. 1998. Binding of hepatitis C virus to CD81. Science 282: 938-941.
26. Ploss A, et al. 2009. Human occludin is a hepatitis C virus entry factor required for infection of mouse cells. Nature 457:882- 886.
27. Roccasecca R, et al. 2003. Binding of the hepatitis C virus E2 glycoprotein to CD81 is strain specific and is modulated by a complex interplay between hypervariable regions 1 and 2. J. Virol. 77:1856 -1867.
28. Scarselli E, et al. 2002. The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus. EMBO J. 21:5017-5025.
29. van Anken E, et al. 2008. Only five of 10 strictly conserved disulfide bonds are essential for folding and eight for function of the HIV-1 envelope glycoprotein. Mol. Biol. Cell 19:4298-4309.
30. Vieyres G, et al. 2010. Characterization of the envelope glycoproteins associated with infectious hepatitis C virus. J. Virol. 84:10159 -10168.
31. Weiner AJ, et al. 1991. Variable and hypervariable domains are found in the regions of HCV corresponding to the flavivirus envelope and NS1 proteins and the pestivirus envelope glycoproteins. Virology 180:842-848.
32. Whidby J, et al. 2009. Blocking hepatitis C virus infection with recombinant form of envelope protein 2 ectodomain. J. Virol. 83:11078 -11089.
33. Wittig I, Braun HP, Schagger H. 2006. Blue native PAGE. Nat. Protoc. 1:418-428.
34. Yi M, Nakamoto Y, Kaneko S, Yamashita T, Murakami S. 1997. Delineation of regions important for heteromeric association of hepatitis C virus E1 and E2. Virology 231:119 -129.
35. Zheng A, et al. 2007. Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus. J. Virol. 81:12465-12471.