HPTLC methods to assay active ingredients in pharmaceutical formulations: A review of the method development and validation steps

Journal of Pharmaceutical and Biomedical Analysis

Volumn 66, Issue , 2012, Pages 11-23

  Shewiyo, D H ^a,b,c   Kaale, E ^b   Risha, P G ^b   Dejaegher, B ^c   Smeyers Verbeke, J ^c   Heyden, Y Vander ^c  

^a Tanzania Food and Drugs Authority   (Tanzania)

^b MUHIMBILI UNIVERSITY OF HEALTH AND ALLIED SCIENCES   (Tanzania)

^c VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Active ingredient; HPTLC; Method development; Method validation; Pharmaceutical analysis; Pharmaceutical formulation

Indexed keywords

ARTEMISININ; CEFETAMET; CLOPIDOGREL; CLOTRIMAZOLE; COTRIMOXAZOLE; DOMPERIDONE; DULOXETINE; EFAVIRENZ; EMTRICITABINE PLUS TENOFOVIR DISOPROXIL; FAMOTIDINE; IBUPROFEN; ISONIAZID PLUS RIFAMPICIN; ITOPRIDE; LAMIVUDINE PLUS NEVIRAPINE PLUS STAVUDINE; LAMIVUDINE PLUS ZIDOVUDINE; LEVOCETIRIZINE; MONTELUKAST; NEBIVOLOL; PARACETAMOL; PIROXICAM; RABEPRAZOLE; RANOLAZINE; RISPERIDONE; ROPINIROLE; STEROID; SUMATRIPTAN; TACROLIMUS; TRANDOLAPRIL; TRIGONELLINE; UNINDEXED DRUG;

ACCURACY; ANALYTIC METHOD; CALIBRATION; DENSITOMETRY; DRUG FORMULATION; DRUG MONITORING; FLUORESCENCE; HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MASS SPECTROMETRY; NONHUMAN; PLANAR CHROMATOGRAPHY; PRIORITY JOURNAL; REVIEW; SEPARATION TECHNIQUE; THIN LAYER CHROMATOGRAPHY; ULTRAVIOLET RADIATION; VALIDATION PROCESS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, THIN LAYER; DENSITOMETRY; HUMANS; PHARMACEUTICAL PREPARATIONS; VALIDATION STUDIES AS TOPIC;

EID: 84861223769     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2012.03.034     Document Type: Review

References (110)

1. Thin Layer Chromatography, Macherey-Nagel, available at (accessed on 10th December 2011). http://separations.co.za/fileadmin/themes/default/pdf/2.%20PRODUCT%20LINES/N.%20MACHEREY-NAGEL/iv.%20CHROMATOGRAPHY/4.%20TLC/MN%20TLC%20Catalogue.pdf.
2. Sherma J. Planar chromatography, review: planar chromatography. Anal. Chem. 2008, 80:4243-4267.
3. Kalasz H., Bathori M. Pharmaceutical applications of TLC. LC-GC Eur. 2001, 10:311-321.
4. Sethi P.D. Quantitative Analysis of Pharmaceutical Formulations, High Performance Thin Layer Chromatography 1996, CBS Publishers and Distributors, New Delhi, India.
5. Poole C.F. Layer chromatography: challenges and opportunities. J. Chromatogr. A 2003, 1000:963-984.
6. Nyiredy S. Planar chromatographic method development using the PRISMA optimization system and flow charts. J. Chromatogr. Sci. 2002, 40:553-563.
7. Kaale E., Risha P., Layloff T. An interlaboratory investigation on the use of high-performance thin layer chromatography to perform assay of Lamivudine-Zidovudine, Metronidazole, Nevirapine and Quinine composite samples. J. Chromatogr. A 2011, 1218(19):2732-2736.
8. Nyiredy S. Chromatography. Joutrnal of Chromatography Library 1992, vol. 51A:109-150. Elsevier, Amsterdam. 5th ed. E. Heftmann (Ed.).
9. G. Morlock, HPTLC steps from application to quantification: workshop presentation, available at (accessed on 25th August 2011). http://www.hptlc.com/pdf/2006pdf/workshop/Morlock%20Workshop%201%20Part%201.pdf.
10. Gocan S. Stationary phases for thin-layer chromatography. J. Chromatogr. Sci. 2002, 40:538-549.
11. Sherma J., Touchstone J.C., Dobbins M.F. Materials and Methods Densitometry in Thin Layer Chromatography: Practice & Applications 1979, 25-120. John Wileys & Sons, USA. J. Sherma, J.C. Touchstone (Eds.).
12. Scott R.M. The stationary phase in thin layer chromatography. J. Chromatogr. Sci. 1973, 2:129-135.
13. Gocan S., Cimpan G., Muresan L. Automated multiple development thin-layer chromatography of some plants extracts. J. Pharm. Biomed. Anal. 1996, 14:1221-1227.
14. Gandhi D.B., Mehta P.J. Simultaneous RP-HPTLC method for determination of levodaopa, carbidopa and entocapone in combined tablet dosage form. J. Planar. Chromatogr.-Mod. TLC 2011, 24:236-241.
15. Shewiyo D.H., Kaale E., Ugullum C., Sigonda M.N., Risha P.G., Dejaegher B., Smeyers-Verbeke J., Vander Heyden Y. Development and validation of a normal-phase HPTLC method for the simultaneous analysis of Lamivudine, Stavudine and Nevirapine in fixed dose combination tablets. J. Pharm. Biomed. Anal. 2011, 54:445-450.
16. Antic D., Filipic S., Agbaba D. A simple and sensitive TLC method for determination of Clopidogrel and its impurity SR 26334 in pharmaceutical products. Acta Chromatogr. 2007, 18:199-206.
17. Vikas R.J.R., Sathiyanarayan L., Yadav S.S. Stability indicating HPTLC method for Trandolapril estimation in the bulk drug and dosage form. Indian J. Educ. Res. 2010, 44:341-344.
18. Bari S.B., Bakhshi A.R., Jain P.S., Surana S.J. Development and validation od stability - indicating TLC - denstitometric determination of Ropinirole hydrochloride in bulk and pharmaceutical dosage form. Pharm. Anal. Acta 2011, 2:2-7.
19. Kaale E., Risha P., Reich E., Layloff T. An interlaboratory investigation on the use of high-performance thin-layer chromatography to perform assays lamivudine-zidovudine, metronidazole, nevirapine, and quinine composite samples. J. AOAC Int. 2010, 93:1836-1843.
20. Anbazhagan S., Indumathy N., Shanmugapandigayan P., Sridhar S.K. Simultaneous quantification of Stavudine, Lamivudine and Nevirapine by UV spectroscopy, reversr phase HPLC and HPTLC in tablets. J. Pharm. Biomed. Anal. 2005, 39:801-804.
21. Rathore A.S., Sathiyanarayanan L., Mahadik K.R. Develeopment of validated HPLC and HPTLC methods for simultaneous determination of levocetirizine dihydrochloride and montelukast sodium in bulk and pharmaceutical dosage form. Pharm. Anal. Acta - Open Access J. 2010, 1:1-6. 10.4172/2153-2435.1000106.
22. Shirkhedkar A.A., Bugdane P.M., Surana S.J. Stability - indicating TLC - densitometric determination of nebivolol hydrochloride in bulk and pharmaceutical dosage form. J. Chromatogr. Sci. 2010, 48:102-113.
23. Shirkhedkar A.A., Shaikh A.M., Surana S.J. Simultaneous determination of paracetamol and piroxicam in tablets by thin-layer chromatography combined with densitometry. Eurasian J. Anal. Chem. 2008, 3:258-267.
24. Borhade V.B., Nair H.A., Hegde D.D. Development and validation of HPTLC method for estimation of tracolimus in formulations. Drug Dev. Ind. Pharm. 2009, 35:440-448.
25. Chakraborty R., Pal K., Shaiba M., Sangepu N., Sridevi P. High performance thin-layer chromatographic estimation of ranolazine. Res. J. Pharm. Biol. Chem. Sci. 2010, 4:152-157.
26. Chitlange S.S., Kumar N., Wankhede S.B. Stability - indicating HPTLC method for estimation of dexibuprofen in pharmaceutical dosage form. J. Pharm. Res. 2009, 9:1542-1546.
27. Chopra S., Ahmad F.J., Khar R.K., Motwani S.K., Mahdi S., Iqbal Z., Talegaonkar S. Validated high performance thin-layer chromatography method for determination of trigonelline in herbal extract and pharmaceutical dosage form. Anal. Chim. Acta 2006, 577:46-51.
28. Cimpoiu C., Hosu A., Hodisan S. Analysis of some steroids by thin-layer chromatography using optimum mobile phases. J. Pharm. Biomed. Anal. 2006, 41:633-637.
29. Deshpande P., Gandhi S., Bhavnani V., Bandewar R., Dhiware A., Diwale V. High performance thin-layer chromatographic determination of famotidine and domperidone in combined tablet dosage form. Res. J. Pharm. Biol. Chem. Sci. 2010, 4:354-359.
30. Devala Rao G., Kathirvel S., Satyanarayana S.V. Development and validation of TLC-Densitometry method for the estimation of Anti-psychotic drug in bulk and tablet formulation. Int. J. ChemTech Res. 2010, 2:2063-2069.
31. Dhaneshwar S.S., Deshpande P., Patil M., Vadnekar G., Dhaneshwar S.R. Development and validation of a HPTLC method for estimation of duloxetine hydrochloride in bulk drug and in tablet dosage form. Indian J. Pharm. Sci. 2008, 70:233-236.
32. Shah C.R., Suhagia B.N., Shah N.J., Shah R.R. Development and validation of a HPTLC method for the estimation of sumatriptan in tablet dosage forms. Indian J. Pharm. Sci. 2008, 70:831-834.
33. Parmar P., Mehta A. Development and validation of HPTLC method for the estimation of Clotrimazole in bulk drug and tablet formulations. Indian J. Pharm. Sci. 2009, 71:451-454.
34. Joshi M., Nikalje A.P., Shahed M., Dehghan M. HPTLC method for the simultaneous estimation of emtricitabine and tenovofiv in tablet dosage forms. Indian J. Pharm. Sci. 2009, 71:95-97.
35. Suganth A., John S., Ravi T.K. Simultaneous HPTLC determination of rabeprazole and itopride hydrochloride from their combined dosage form. Indian J. Pharm. Sci. 2008, 70:366-368.
36. Habte G., Hymete A., Mohammed A.M.I. Simultaneous separation and determination of lamivudine and zidovudine in pharmaceutical formulations using the HPTLC method. Anal. Lett. 2009, 42:1552-1570.
37. Shewiyo D.H., Kaale E., Risha P.G., Dejaegher B., Smeyers-Verbeke J., Vander Heyden Y. Development and validation of a normal-phase high-performance thin layer chromatographic method for the analysis of Sulfamethoxazole and Trimethoprim in Co-trimoxazole tablets. J. Chromatogr. A 2009, 1216:7102-7107.
38. Ali J., Ali N., Sultana Y., Baboota S., Faiyaz S. Development and validation of a stability indicating HPTLC method for analysis of antitubercular drugs. Acta Chromatogr. 2007, 13:168-179.
39. Potale L.V., Khodke A.S., Patole S.M., Damle M.C. Development and validation of stability - indicating HPTLC method for determination of efavirenz as bulk drug and in pharmaceutical formulation. J. Adv. Pharm. Res. 2010, 1:115-122.
40. Starek M., Krzek J. TLC chromatographic-densitometric assay of ibuprofen and its impurities. J. Chromatogr. Sci. 2010, 48:825-829.
41. Krzek J., Kwiecien A. Application of densitometry for determination of beta-adrenergic-blocking agents in pharmaceutical preparations. J. Planar Chromatogr. 2005, 18:308-313.
42. Kshirsagar V.B., Deokate U.A., Bharkad V.B., Khadabadi S.S. HPTLC method development and validation for the simultaneous estimation of diosgenin and levodopa in marketed formulation. Asian J. Res. Chem. 2008, 1:36-39.
43. Lakshmana Rao A., Prudhvi N., Basavaraj K.P.C., Manohara Y.N. Development and validation of a HPTLC method for the estimation of cefetamet. Rasayan J. Chem. 2009, 2:720-723.
44. Mahadik M.V., Dhaneshwar S.R. Application of stability - indicating HPTLC method for the quantitative determination of ezetimibe in pharmaceutical dosage forms. Asian J. Pharm. Sci. 2007, 5:182-190.
45. Agarwala S.P, Ali A., Dua Y., Ahuja S. Determinatin of Artemisinin in bulk and pharmaceutical dosage forms using HPTLC. Indian J. Pharm. Sci. 2009, 71:98-100.
46. Saleh G.A., Mohamed F.A., El-Shaboury S.R., Rageh A.H. Selective densitometric determination of four α-aminocephalosporins using ninhydrin reagent. J. Chromatogr. Sci. 2010, 48:68-75.
47. Patel B.H., Suhagia B.N., Patel M.M., Patel J.R. HPTLC determination of rabeprazole and domperidone in capsules and its validation. J. Chromatogr. Sci. 2008, 46:304-307.
48. Patel R.B., Patel M.R., Bhatt K.K., Patel B.G. Development and validation of a HPTLC method for estimation of Carbamazepine in formulations and is in vitro release study. Chromatogr. Res. Int. 2011, 2011:1-8.
49. Patel V.A., Patel P.G., Chaudhary B.G., Rajgor N.B., Rathi S.G. Development and validation of HPTLC method for the simultaneous estimation of telmisartan and ramipril in combined dosage form. Int. J. Pharm. Biol. Res. 2010, 1:18-24.
50. Dhaneshwar S.S., Daneshwar S.R., Deshpande P., Patil M. Development anda validation of a method for the densitometric estimation of atorvastatin calcium and ezetimibe as the bulk drugs and tablet dosage forms. Acta Chromatogr. 2007, 19:141-148.
51. Ranjane P.N., Gandhi S.V., Kadur S.S., Bothara K.G. HPTLC determination of cefuroxime axetil and ordinazole in combined tablet dosage forms. J. Chromatogr. Sci. 2010, 48:26-28.
52. Kumbhar S.T., Chougule G.K., Tegeli V.S., Gajeli G.B., Thorat Y.S., Shivsharan U.S. A validated HPTLC method for simultaneous quantification of of nevibolol and hydrochlorothiazide in bulk and tablet formulation. Int. J. Pharm. Sci. Drug Res. 2011, 3:62-66.
53. Dhaneshwar S.R., Bhusari V.K., Mahadik M.V., Santakumari B. Application of a stability-indicating thin-layer chromatographic method to the determination of tenatoprazole in pharmaceutical dosage forms. J. AOAC Int. 2009, 92:387-393.
54. Meshram D.B., Bagade S.B., Tajne M.R. A simple TLC method for analysis of fluconazole in pharmaceutical dosage forms. J. Planar Chromatogr. 2008, 21:191-195.
55. Ebrahim Z.A.J., Balalau D., Baconi D.L., Gutu C.M., Ilie M. HPTLC method for the assay of tramadol and pentazocin from mixtures. Farmacia 2011, 59:381-387.
56. Anand M., Fonseca A., Santosh V.G., Deshpande B.P. Development and validation of a high-performance thin-layer chromatographic method for estimation of brimodine tartrate as bulk drug and ophthalmic solutions. Int. J. ChemTech Res. 2010, 2:1376-1379.
57. Chandra P., Rathore A.S., Sathiyanarayanan L., Mahadik K.R. Application of high-performance thin-layer chromatographic method for the simultaneous determination of Lamivudine and tenofovir disoproxil fumarate in pharmaceutical dosage form. J. Chil. Chem. Soc. 2011, 56:702-705.
58. Shanmugasundaram P., Maheswari R., Vijayaanandhi M. Quantitative estimation of piperine in herbal cough syrup by HPTLC method. Rayasan J. Chem. 2008, 1:212-217.
59. Jain P.S. Stability - indicating HPTLC determination of ambroxol hydrochloride in bulk drug and pharmaceutical dosage form. J. Chromatogr. Sci. 2010, 48:45-48.
60. Kwiecien A., Krzek J., Biniek L. TLC - densitometric determination of azithromycin in pharmaceutical preparations. J. Planar Chromatogr. 2008, 21:177-181.
61. Esters V., Angenot L., Brandt V., Frederich M., Tits M., Van Nerum C., Wauters J-N, Hubert Ph. Validation of a high-performance thin-layer chromatography/densitometry method for the quantitative determination of glucosamine in a herbal dietary supplement. J. Chromatogr. A 2006, 1112:156-164.
62. Gubitz G., Schmid M.G. Chiral separation by chromatographic and electromigration techniques. Biopharm. Drug Dispos. 2001, 22:291-336.
63. Hauck H.E., Schulz M. Ultrathin-layer chromatography. J. Chromatogr. Sci. 2002, 40:1-3.
64. Geiss F. Fundamentals of Thin Layer Chromatography (Planar Chromatography) 1987, Dr. Alfred Huthig Verlag, Heidelberg, Germany.
65. Snyder L.R. Classification of the solvent properties of common liquids. J. Chromatogr. Sci. 1978, 16:223-233.
66. Nyiredy S. Essential Guide to Method development in Thin-Layer (Planar) Chromatography 2006, Academic Press, Budakalasz, Hungary, pp. 4652-4666.
67. Touchstone J.C. Practice of Thin Layer Chromatography 1992, John Wiley & Sons, New York. 3rd ed.
68. Massart D.L., Vandeginste B.G.M., Buydens L.M.C., De Jong S., Lewi P.J., Smeyers-Verbeke J. Handbook of Chemometrics and Qualimetrics: Part A 1997, Elsevier, Amsterdam.
69. Canales M. Liquid Chromatography in Pharmaceutical Development 1985, 181. Aster Publishing, USA. I.W. Wainer (Ed.).
70. Y. Vander Heyden, LC.LG Asia Pacific (2006), available at (accessed on 12th December 2011). http://chromatographyonline.findanalytichem.com/lcgc/article/articleDetail.jsp?id=445076&sk=&date=&pageID=2.
71. Nyiredy S. Chromatography. Joutrnal of Chromatography Library 2004, vol. 69A:253-296. Elsevier, Amsterdam. 6th ed. E. Heftmann (Ed.).
72. Ekiert R.J., Krzek J., Rzesulko W. Evaluation of a TLC densitometric method for analysis of azole atifungal agents. Chromatographia 2008, 67:995-998.
73. Cimpoiu C., Hodisan T. Application of numerical taxonomy techniques to the choice of optimum mobile phase in high-performance thin-layer chromatography (HPTLC). J. Pharm. Biomed. Anal. 1999, 21:895-900.
74. The system Camag Nanomat and Capillary Dispensor, Camag, Switzerland, available at (assessed on 21st June 2011). http://www.kobis.si/material/Nanomat4brochure.pdf.
75. Linomat 5, Bandwise sample application by spray-on technique for planar chromatography, Camag, Switzerland, (accessed on 2nd January 2012). http://www.instrument.com.cn/download.asp%3Furl.pdf.
76. The Instrumental thin-layer chromatography, Catalogue 2010/11, Camag, Switzerland, available at (accessed on 21st June 2011). http://www.camag.com/downloads/free/brochures/CAMAG_TLC10-11_E.pdf.
77. Brainthwaite S., Smith F.J Chromatographic Methods 1999, Kluwer Academic Publishers, London. 5th ed.
78. Matysic G., Soczewinski E. Stepwise gradient development in thin-layer chromatography: II. Two-dimensional gradients for complex mixtures. J. Chromatogr. A 1986, 369:19-25.
79. Nyiredy S. The bridge between TLC and HPLC: overpressured layer chromatography, (OPLC). Trends Anal. Chem. 2001, 20:91-100.
80. Nyiredy S. Progress in forced-flow planar chromatography. J. Chromatogr. A 2003, 1000:985-999.
81. Katona Z., Vincze L., Vegh Z., Trompler A., Forenczi-Fodor K. Cleaning validation procedure eased by using overpressured layer chromatography. J. Pharm. Biomed. Anal. 2000, 22:349-353.
82. Vovk I., Prosek M. Reproducibility of densitometric and image analyzing quantitative evaluation of thin-layer chromatograms. J. Chromatogr A 1997, 770:329-336.
83. Ebel S., Henkel T. Evaluation in HPTLC by use of imaging system. 1. Basic algorithms of image processing. J. Planar Chromatogr. 2000, 13:248-253.
84. G. Morlock, HPTLC-MS and other coupling techniques, available at (accessed on 15th September 2011). http://nte-serveur.univ-lyon1.fr/afsep/fichier/271_14_11_2005.pdf.
85. T. Yrjonen, Extraction and planar chromatographic separation technique in the analysis of natural products, Academic dissertation, Faculty of Pharmacy, University of Helsinki, November 2004.
86. Jautz U., Morlock G. Efficacy of planar chromatography coupled to (tandem) mass spectrometry for employment in trace analysis. J. Chromatogr. A 2006, 1128:244-250.
87. G. Morlock, Hyphenations in HPTLC with UV/vis/FLD, MS, FTIR, NMR and bioassays, available at (accessed on 15th January 2012). http://www.ilc.uni-hohenheim.de/joomla/images/stories/GM/Flyer%20TLC-MS%20Interface.pdf.
88. Tuzimski T. Application of different modes of thin-layer chromatography and mass spectrometry for the separation and detection of large and small biomolecules. J. Chromatogr. A 2011, 1218:8799-8812.
89. Funk W., Dammann V., Donnevert G. Quality Assurance in Analytical Chemistry 2007, Wiley-VCH, Weinheim. 2nd ed.
90. Guidelines prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human use (ICH), Validation of Analytical Procedures: Text and Methodology, Q2 (R1), 2005, 1-13 (accessed on August 20th 2011). http://www.ich.org/.
91. Food and Drugs Administration, Guidance for Industry, Analytical Procedures and Method Validation (Draft Guidance), 2000, available at (accessed on 25th August 2011). http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/ucm122858.pdf.
92. The United States Pharmacopeia 29, National Formulary 24, Unites States Pharmacopeia Convention, Rockville MD USA, 2006.
93. AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals, available at (accessed on 16th September 2011). http://www.aoac.org/Official_Methods/slv_guidelines.pdf.
94. Ferenczi-Fodor K., Renger B., Vegh Z. The frustrated reviewer-Recurrent failures in manuscripts describing validation of quantitative TLC/HPTLC procedures for analysis of pharmaceuticals. J. Planar Chromatogr. 2010, 23:173-179.
95. Van Loco J., Elskens M., Croux C., Beernaert H. Linearity of calibration curves: use and misuse of correlation coefficient. Accred. Qual. Assur. 2002, 7:281-285.
96. EURACHEM Guide, The Fitness for Purpose of Analytical Methods, 1998, available at (accessed on 25th August 2011). http://www.eurachem.org/guides/pdf/valid.pdf.
97. Kulikov A.U., Verushkin A.G., Loginova L.P. Chromatographia 2005, 61:455-463.
98. Rozet E., Marini R.D., Ziemons E., Boulanger B., Hubert Ph. Advance in validation, risk and uncertainty assessment of bioanalytical methods. J. Pharm. Biomed. Anal. 2011, 55:848-858.
99. International Organization for Standardization, Accuracy (Trueness and Precision) of Measurement Methods and Results, ISO:DIS 5725-1 to 5725-3, Geneva, 1994.
100. Hubert Ph., Nguyen-Huu J.-J., Boulanger B., Chapuzet E., Chiap P., Cohen N., Compagnon P.-A., Dewe W., Feinberg M., Lallier M., Laurentie M., Mercier N., Muzard G., Nivet C., Valat L., Rozet E. Harmonization of strategies for the validation of quantitative analytical procedures: a SFSTP proposal - Part II. J. Pharm. Biomed. Anal. 2007, 45:70-81.
101. Hartmann C., Smeyers-Verbeke J., Pennickx W., Vander Heyden Y., Vankeerberghen P., Massart D.L. Reappraisal of hypothesis testing for method validation: detection of systematic error by comparing the means of two or of two laboratories. Anal. Chem. 1995, 67:4491-4499.
102. Hubert Ph., Nguyen-Huu J.-J., Boulanger B., Chapuzet E., Chiap P., Cohen N., Compagnon P.-A., Dewe W., Feinberg M., Lallier M., Laurentie M., Mercier N., Muzard G., Nivet C., Valat L. Harmonization of strategies for the validation of quantitative analytical procedures: a SFSTP proposal - Part I. J. Pharm. Biomed. Anal. 2004, 36:579-586.
103. Hubert Ph., Nguyen-Huu J.-J., Boulanger B., Chapuzet E., Cohen N., Compagnon P.-A., Dewe W., Feinberg M., Laurentie M., Mercier N., Muzard G., Valat L., Rozet E. Harmonization of strategies for the validation of quantitative analytical procedures: A SFSTP proposal - Part III. J. Pharm. Biomed. Anal. 2007, 45:82-96.
104. Ferenczi-Fodor K., Vegh Z., Renger B. Thin-layer chromatography in testing the purity of pharmaceuticals. Trend. Anal. Chem. 2006, 25:778-789.
105. Machale V.P., Gatade A.T., Sane R.T. Validated HPTLC content uniformity test for the determination of diazepam in tablet dosage form. Int. J. Pharm. Res. Dev. 2011, 3:34-41.
106. Morlock G.E. Analysis of pesticide residuals in drinking water by planar chromatography. J. Chromatogr. A 1996, 754:423-430.
107. Hassan M., Ahmad F., Sagheer M., Iqbal M.F., Tariq M. Residual persistence of chlorpyrifos, imidacloprid, and acephate in brinjal fruit. Pak. Entomol. 2005, 27:53-55.
108. Morlock G.E., Schwack W. The contribution of planar chromatography to food analysis. J. Planar Chromatogr. 2007, 20:399-406.
109. Mennickent S., Fierro R., Vega M., De Diego M., Godoy C.G. Quantitative determination of fluoxetine in huma serum by high-performance thin-layer chromatography. J. Sep. Sci. 2010, 33:2206-2210.
110. Jain G.K., Jain N., Iqbal Z., Talegaonkar S., Ahmad F.J., Khar R.K. Development and validation of an HPTLC method for the determination of minocycline in human plasma. Acta Chromatogr. 2007, 19:197-205.