Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells

PLoS ONE

Volumn 7, Issue 5, 2012, Pages

  Kuroda, Takuya ^a,b   Yasuda, Satoshi ^a   Kusakawa, Shinji ^a,b   Hirata, Naoya ^a   Kanda, Yasunari ^a   Suzuki, Kazuhiro ^a   Takahashi, Masayo ^b,c   Nishikawa, Shin Ichi ^b,c   Kawamata, Shin ^b   Sato, Yoji ^a,b,d  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^b FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION   (Japan)

^c RIKEN Center for Biosystems Dynamics Research   (Japan)

^d NAGOYA CITY UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; LIN28 PROTEIN; MESSENGER RNA; TRA 1 60 ANTIGEN; UNCLASSIFIED DRUG; LIN 28 PROTEIN, HUMAN; LIN-28 PROTEIN, HUMAN; RNA BINDING PROTEIN;

ARTICLE; ASSAY; CELL DIFFERENTIATION; CELL SURVIVAL; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IN VITRO STUDY; LIMIT OF DETECTION; PIGMENT EPITHELIUM; PLURIPOTENT STEM CELL; QUANTITATIVE ANALYSIS; QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOFT AGAR COLONY FORMATION ASSAY; BIOSYNTHESIS; CARCINOGEN TESTING; CYTOLOGY; EMBRYONIC STEM CELL; EVALUATION; METHODOLOGY;

ANIMALIA; IPS;

CARCINOGENICITY TESTS; CELL DIFFERENTIATION; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; HUMANS; INDUCED PLURIPOTENT STEM CELLS; REAL-TIME POLYMERASE CHAIN REACTION; RETINAL PIGMENT EPITHELIUM; RNA, MESSENGER; RNA-BINDING PROTEINS; SENSITIVITY AND SPECIFICITY;

EID: 84861215843     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0037342     Document Type: Article

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