Approaches for improving thermostability characteristics in cellulases

Methods in Enzymology

Volumn 510, Issue , 2012, Pages 261-271

  Anbar, Michael ^a   Bayer, Edward A ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

Consensus mutagenesis; Directed evolution; Error prone PCR; Glycoside hydrolases; Screening; Thermostability

Indexed keywords

BETA GLUCOSIDASE; CELLULASE; DNA; GLUCAN SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; CLOSTRIDIUM THERMOCELLUM; DNA LIBRARY; DNA RECOMBINATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; IN VITRO STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; THERMOSTABILITY;

EID: 84861158254     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-415931-0.00014-8     Document Type: Chapter

References (9)

1. N. Amin, M. Roberge, M. Estabrook, J. Basler, R. Chin, P. Gualfetti, A. Liu, S.B. Wong, M.H. Rashid, T. Graycar, L. Babé, and V. Schellenberger Construction of stabilized proteins by combinatorial consensus mutagenesis Protein Eng. Des. Sel. 17 2004 787 793
2. M. Anbar, M. Anbar, R. Lamed, and E.A. Bayer Thermostability enhancement of Clostridium thermocellum cellulosomal endoglucanase Cel8A by a single glycine substitution ChemCatChem. 2 2010 997 1003
3. A. Herman, and D.S. Tawfik Incorporating synthetic oligonucleotides via gene reassembly (ISOR): A versatile tool for generating targeted libraries Protein Eng. Des. Sel. 20 2007 219 226
4. M. Lehmann, C. Loch, A. Middendorf, D. Studer, S.F. Lassen, L. Pasamontes, A.P. van Loon, and M. Wyss The consensus concept for thermostability engineering of proteins: Further proof of concept Protein Eng. 15 2002 403 411
5. G.L. Miller Use of dinitrosalicylic acid reagent for determination of reducing sugar Anal. Biochem. 31 1959 426 428
6. K.M. Polizzi, J.F. Chaparro-Riggers, E. Vazquez-Figueroa, and A.S. Bommarius Structure-guided consensus approach to create a more thermostable penicillin G acylase Biotechnol. J. 1 2006 531 536
7. B. Steipe, B. Schiller, A. Plückthun, and S. Steinbacher Sequence statistics reliably predict stabilizing mutations in a protein domain J. Mol. Biol. 240 1994 188 192
8. T. Wood, and K. Bhat Methods for measuring cellulases activities Methods Enzymol. 160 1988 87 112
9. Y.H. Zhang, M.E. Himmel, and J.R. Mielenz Outlook for cellulase improvement: Screening and selection strategies Biotechnol. Adv. 24 2006 452 481