Measurement of the activity of individual subunits of single molecules of the tetrameric enzyme β-galactosidase

Analytical Chemistry

Volumn 84, Issue 10, 2012, Pages 4598-4602

  Craig, Douglas B ^a   Morris, Thomas T ^a   Ong Justiniano, Coleen Marie Q ^b  

^a UNIVERSITY OF WINNIPEG   (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CONTINUOUS FLOWS; ENZYME MOLECULES; FLUOROGENIC SUBSTRATE; GALACTOSIDASES; INHIBITOR CONCENTRATION; SINGLE MOLECULE; SLOW RELEASE; STEPWISE INCREASE; TETRAMERIC ENZYMES;

CAPILLARY ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI;

MOLECULES;

BETA GALACTOSIDASE; ENZYME INHIBITOR; ESCHERICHIA COLI PROTEIN; GALACTOSIDE;

ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; ENZYME ASSAY; ENZYMOLOGY; ESCHERICHIA COLI; KINETICS; METABOLISM; PROTEIN MULTIMERIZATION;

BETA-GALACTOSIDASE; ELECTROPHORESIS, CAPILLARY; ENZYME ASSAYS; ENZYME INHIBITORS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GALACTOSIDES; KINETICS; PROTEIN MULTIMERIZATION;

EID: 84861136238     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac300777u     Document Type: Article

References (17)

1. Xue, Q.; Yeung, E. S. Nature 1995, 373, 681-683
2. Craig, D. B.; Arriaga, E. A.; Wong, J. C. Y.; Lu, H.; Dovichi, N. J. J. Am. Chem. Soc. 1996, 118, 5245-5253
3. Lu, H. P.; Xun, L.; Xie, S. Science 1999, 282, 1877-1882
4. Edman, L.; Foldes-Papp, Z.; Wennmalm, S.; Rigler, R. Chem. Phys. 1999, 247, 11-22
5. Craig, D. B.; Haslam, A. M.; Coombs, J. M. L.; Nichols, E. R. Biochem. Cell Biol. 2010, 88, 451-458
6. Nichols, E. R.; Craig, D. B. Electrophoresis 2008, 29, 4257-4269
7. Tan, W.; Yeung, E. S. Anal. Chem. 1997, 69, 4242-4248
8. Jacobson, R. H.; Zhang, X.-J.; DuBose, R. F.; Matthews, B. W. Nature 1994, 369, 761-766
9. Craig, D. B.; Nichols, E. R. Electrophoresis 2008, 29, 4298-4303
10. Lee, Y. C. Biochem. Biophys. Res. Commun. 1969, 35, 161-167
11. Wentworth, D. F.; Wolfenden, R. Biochemistry 1974, 13, 4715-4720
12. Gorris, H.-H.; Rissen, D. M.; Walt, D. R. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 17680-17685
13. Craig, D. B.; Chase, L. N. Anal. Chem. 2012, 84, 2044-2047
14. Zhao, J.-Y.; Chen, D.-Y.; Dovichi, N. J. J. Chromatogr. 1992, 608, 117-120
15. Shoemaker, G. K.; Juers, D. H.; Coombs, J. M. L.; Matthews, B. W.; Craig, D. B. Biochemistry 2003, 42, 1707-1710
16. Craig, D. B.; Schwab, T.; Sterner, R. Biochem. Cell Biol. 2012, in press.
17. Monod, J.; Wyman, J.; Changeux, J.-P. J. Mol. Biol. 1965, 12, 88-118