Efficient formation of cell spheroids using polymer nanofibers

Biotechnology Letters

Volumn 34, Issue 5, 2012, Pages 795-803

  Shin, Jung Youn ^a   Park, Jooyeon ^a   Jang, Hyeon Ki ^a   Lee, Tae Jin ^b   La, Wan Geun ^a   Bhang, Suk Ho ^a   Kwon, Il Keun ^c   Kwon, Oh Hyeong ^d   Kim, Byung Soo ^a  

^a Seoul National University   (South Korea)

^b Hanyang University   (South Korea)

^c Kyung Hee University   (South Korea)

^d Kumoh National Institute of Technology   (South Korea)

Author keywords

Anchorage dependent cells; Human dermal fibroblast; Human embryonic kidney 293 cells; Polymer nanofiber; Spheroid suspension culture

Indexed keywords

293 CELLS; ANCHORAGE-DEPENDENT CELLS; HUMAN DERMAL FIBROBLASTS; POLYMER NANOFIBERS; SUSPENSION CULTURES;

ANIMAL CELL CULTURE; BIOMATERIALS; CELL ADHESION; CELL DEATH; POLYMERS;

NANOFIBERS;

LACTIC ACID; NANOFIBER; POLYGLYCOLIC ACID; POLYLACTIC ACID POLYGLYCOLIC ACID COPOLYMER; POLYLACTIC ACID-POLYGLYCOLIC ACID COPOLYMER;

ARTICLE; CULTURE TECHNIQUE; DRUG EFFECT; HUMAN; METABOLISM; METHODOLOGY; MULTICELLULAR SPHEROID;

CELL CULTURE TECHNIQUES; HUMANS; LACTIC ACID; NANOFIBERS; POLYGLYCOLIC ACID; SPHEROIDS, CELLULAR;

ANIMALIA;

EID: 84860886953     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0836-9     Document Type: Article

References (15)

1. Choi WS, Bae JW, Lim HR, Joung YK, Park JC, Kwon IK, Park KD (2008) RGD peptide-immobilized electrospun matrix of polyurethane for enhanced endothelial cell affinity. Biomed Mater 3: 044104.
2. Geiger B, Ayalon O (1992) Cadherins. Annu Rev Cell Biol 8: 307-332.
3. Hu WS, Aunins JG (1997) Large-scale mammalian cell culture. Curr Opin Biotechnol 8: 148-153.
4. Kallos MS, Behie LA (1999) Inoculation and growth conditions for high-cell-density expansion of mammalian neural stem cells in suspension bioreactors. Biotechnol Bioeng 63: 473-483.
5. Lin RZ, Chang HY (2008) Recent advances in three-dimensional multicellular spheroid culture for biomedical research. Biotechnol J 3: 1172-1184.
6. Meredith JE Jr, Schwartz MA (1997) Integrins, adhesion and apoptosis. Trends Cell Biol 7: 146-150.
7. Meredith JE Jr, Fazeli B, Schwartz MA (1993) The extracellular matrix as a cell survival factor. Mol Biol Cell 4: 953-961.
8. Parsons JT, Martin KH, Slack JK, Taylor JM, Weed SA (2000) Focal adhesion kinase: a regulator of focal adhesion dynamics and cell movement. Oncogene 19: 5606-5613.
9. Peshwa MV, Kyung YS, McClure DB, Hu WS (1993) Cultivation of mammalian cells as aggregates in bioreactors: effects of calcium concentration on spatial distribution of viability. Biotechnol Bioeng 41: 179-187.
10. Ruoslahti E, Reed JC (1994) Anchorage dependence, integrins, and apoptosis. Cell 77: 477-478.
11. Ryu JH, Oh DJ, Choi CY, Kim BS (2003) Suspension culture of anchorage-dependent animal cells using nanospheres of the biodegradable polymer, poly(lactic-co-glycolic acid). Biotechnol Lett 25: 1363-1367.
12. Ryu JH, Kim MS, Lee GM, Choi CY, Kim BS (2005) The enhancement of recombinant protein production by polymer nanospheres in cell suspension culture. Biomaterials 26: 2173-2181.
13. Sinacore MS, Drapeau D, Adamson SR (2000) Adaptation of mammalian cells to growth in serum-free media. Mol Biotechnol 15: 249-257.
14. Thomas P, Smart TG (2005) HEK293 cell line: a vehicle for the expression of recombinant proteins. J Pharmacol Toxicol Methods 51: 187-200.
15. Tolbert R, Hitt MM, Feder J (1980) Cell Aggregate suspension culture for large-scale production of biomolecules. In Vitro 16: 486-490.