Using an E. coli Type 1 secretion system to secrete the mammalian, intracellular protein IFABP in its active form

Journal of Biotechnology

Volumn 159, Issue 3, 2012, Pages 155-161

  Schwarz, Christian K W ^a   Landsberg, Christine D ^a   Lenders, Michael H H ^a   Smits, Sander H J ^a   Schmitt, Lutz ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

FKBP; Haemolysin A; IFABP; Inclusion bodies; Purification protocol; Type 1 secretion

Indexed keywords

FKBP; HAEMOLYSINS; IFABP; INCLUSION BODIES; PURIFICATION PROTOCOL; TYPE 1 SECRETION;

BLOOD; CYTOLOGY; ENZYME ACTIVITY; ESCHERICHIA COLI; MAMMALS; PHYSIOLOGY; PURIFICATION;

PROTEINS;

ALPHA HEMOLYSIN; BACTERIAL PROTEIN; FATTY ACID BINDING PROTEIN; MALTOSE BINDING PROTEIN; MUTANT PROTEIN; PERIPLASMIC PROTEIN;

ANIMAL CELL; ARTICLE; CYTOPLASM; ENZYME PURIFICATION; ESCHERICHIA COLI; EUKARYOTIC CELL; KINETICS; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SECRETION; TYPE I SECRETION SYSTEM;

BACTERIAL SECRETION SYSTEMS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FATTY ACID-BINDING PROTEINS; HEMOLYSIN PROTEINS; INCLUSION BODIES; PROTEIN BINDING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE;

BACTERIA (MICROORGANISMS); EUKARYOTA; MAMMALIA;

EID: 84860833922     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.02.005     Document Type: Article

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