Ex vivo mimicry of normal and abnormal human hematopoiesis

Journal of Visualized Experiments

Volumn , Issue 62, 2012, Pages

  Mortera Blanco, Teresa ^a   Rende, Maria ^a   Macedo, Hugo ^a   Farah, Serene ^a   Bismarck, Alexander ^a   Mantalaris, Athanasios ^a   Panoskaltsis, Nicki ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Bioengineering; Cord blood; Hematopoiesis; Issue 62; Leukemia; Three dimensional culture

Indexed keywords

EID: 84860670298     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3654     Document Type: Article

References (13)

1. Orkin, S. & Zon, L. Hematopoiesis: an evolving paradigm for stem cell biology. Cell. 132, 631-644 (2008).
2. Spradling, A., Drummond-Barbosa, D., & Kai, T. Stem cells find their niche. Nature. 414, 98-104 (2001).
3. Panoskaltsis, N., Mantalaris, A., & Wu, D. Engineering a mimicry of bone marrow tissue ex vivo. J Biosci. Bioeng. 100, 28-35 (2005).
4. Lo Celso, C., et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. Nature. 457, 92-96 (2009).
5. Mantalaris, A., Bourne, P., & Wu, J. Production of human osteoclasts in a three-dimensional bone marrow culture system. Biochem. Eng. J. 20, 189-196 (2004).
6. Placzek, M., et al. Stem cell bioprocessing: fundamentals and principles. J. R. Soc. Interface. 6, 209-232 (2009).
7. Dexter, T. & Testa, N. In: Methods in Cell Biology., Prescott, D., ed., Academic Press Inc, New York, 14, 387-405 (1976).
8. Piacibello, W., et al. Differential growth factor requirement of primitive cord blood hematopoietic stem cell for self-renewal and amplification vs proliferation and differentiation. Leukemia. 12, 718-727 (1998).
9. Yoshida, T. & Takagi, M. Cell processing engineering for ex vivo expansion of hematopoietic cells: a review. Biochemical Engineering Journal. 20, 99-106 (2004).
10. Lim, M., et al. Intelligent bioprocessing for haemotopoietic cell cultures using monitoring and design of experiments. Biotechnol. Adv. 25, 353-368 (2007).
11. Mortera-Blanco, T., Mantalaris, A., Bismarck, A., & Panoskaltsis, N. The development of a three-dimensional scaffold for ex vivo biomimicry of human acute myeloid leukaemia. Biomaterials. 31, 2243-2251 (2010).
12. Mortera-Blanco, T., Mantalaris, A., Bismarck, A., Aqel, N., & Panoskaltsis, N. Long-term cytokine-free expansion of cord blood mononuclear cells in three-dimensional scaffolds. Biomaterials. 32, 9263-9270 (2011).
13. Safinia, L., Datan, N., Hohse, M., Mantalaris, A., & Bismarck, A. Towards a methodology for the effective surface modification of porous polymer scaffolds. Biomaterials. 26, 7537-7547 (2005).