Fluorescence-microscopy screening and next-generation sequencing: Useful tools for the identification of genes involved in organelle integrity

Journal of Visualized Experiments

Volumn , Issue 62, 2012, Pages

  Stefano, Giovanni ^a   Renna, Luciana ^a   Brandizzi, Federica ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Confocal screening; EMS mutagenesis; Genetics; Issue 62; Mapping; Secretory pathway

Indexed keywords

EID: 84860642644     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3809     Document Type: Article

References (16)

1. Marti, L., et al. A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway. The Plant journal: for cell and molecular biology. 63, 901-913, doi:10.1111/j.1365-313X.2010.04296.x (2010).
2. Stefano, G., Renna, L., Moss, T., McNew, J., & Brandizzi, F. In Arabidopsis the spatial and dynamic organization of the endoplasmic reticulum and Golgi apparatus is influenced by the integrity of the c-terminal domain of RHD3, a non-essential GTPase. The Plant Journal. Accepted for publication, (2011).
3. Kim, Y., Schumaker, K.S., & Zhu, J.K. EMS mutagenesis of Arabidopsis. Methods in molecular biology. 323, 101-103, doi:10.1385/1-59745-003-0:101 (2006).
4. Maple, J. & Moller, S.G. Mutagenesis in Arabidopsis. Methods in molecular biology. 362, 197-206 (2007).
5. Greene, E.A., et al. Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics. 164, 731-740 (2003).
6. Krieg, D.R. Ethyl methanesulfonate-induced reversion of bacteriophage T4rII mutants. Genetics. 48, 561-580 (1963).
7. Kovalchuk, I., Kovalchuk, O., & Hohn, B. Genome-wide variation of the somatic mutation frequency in transgenic plants. The EMBO journal. 19, 4431-4438, doi:10.1093/emboj/19.17.4431 (2000).
8. Boulaflous, A., Faso, C., & Brandizzi, F. Deciphering the Golgi apparatus: from imaging to genes. Traffic. 9, 1613-1617, doi:10.1111/j.1600-0854.2008.00769.x (2008).
9. Borevitz, J. Genotyping and mapping with high-density oligonucleotide arrays. Methods in molecular biology. 323, 137-145, doi:10.1385/1-59745-003-0:137 (2006).
10. Konieczny, A. & Ausubel, F.M. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. The Plant journal: for cell and molecular biology. 4, 403-410 (1993).
11. Bell, C.J. & Ecker, J.R. Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics. 19, 137-144, doi:10.1006/geno.1994.1023 (1994).
12. Hazen, S.P. & Kay, S.A. Gene arrays are not just for measuring gene expression. Trends in Plant Science. 8, 413-416 (2003).
13. Hazen, S.P., et al. Rapid array mapping of circadian clock and developmental mutations in Arabidopsis. Plant Physiology. 138, 990-997, doi:10.1104/pp.105.061408 (2005).
14. Bentley, D.R., et al. Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 456, 53-59, doi:10.1038/nature07517 (2008).
15. Weigel, D., & Glazebrook, J. Arabidopsis. A Laboratory Manual. Cold Spring Harbor Laboratory Press., Cold Spring Harbor, New York, (2002).
16. Voelkerding, K.V., Dames, S., & Durtschi, J.D. Next generation sequencing for clinical diagnostics-principles and application to targeted resequencing for hypertrophic cardiomyopathy: a paper from the 2009 William Beaumont Hospital Symposium on Molecular Pathology. J. Mol. Diagn. 12, 539-551, doi:10.2353/jmoldx.2010.100043 (2010).