Engineering characteristics of a single-use stirred bioreactor at bench-scale: The Mobius CellReady 3L bioreactor as a case study

Engineering in Life Sciences

Volumn 11, Issue 4, 2011, Pages 359-368

  Kaiser, Stephan C ^a   Eibl, Regine ^a   Eibl, Dieter ^a  

^a ZURICH UNIVERSITY OF APPLIED SCIENCES   (Switzerland)

Author keywords

CFD; Engineering characteristics; Single use bioreactor

Indexed keywords

ANIMAL CELLS; BIOPHARMACEUTICALS; CELL EXPANSION; ENGINEERING CHARACTERISTICS; ENGINEERING PARAMETERS; MIXING TIME; MULTI-PHASE FLUID FLOW; OPERATION CONDITIONS; OXYGEN MASS TRANSFER; POWER INPUT; PROTEIN PRODUCTION; SINGLE-USE BIOREACTOR; STIRRED BIOREACTORS;

ANIMAL CELL CULTURE; ANIMALS; BIOCONVERSION; COMPUTATIONAL FLUID DYNAMICS; FLOW OF FLUIDS; GROWTH KINETICS; STAINLESS STEEL;

BIOREACTORS;

BIOREACTOR; COMPUTATIONAL FLUID DYNAMICS; MASS TRANSFER; NUMERICAL MODEL; OXYGEN;

ARTICLE; CELL EXPANSION; CELL GROWTH; FLOW RATE; FLUID FLOW; NONHUMAN; SIMULATION; STIRRED REACTOR; VELOCITY;

ANIMALIA;

EID: 84860410382     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201000171     Document Type: Article

References (43)

1. Eibl, R., Kaiser, S., Lombriser, R., Eibl, D., Disposable bioreactors: the current state-of-the-art and recommended applications in biotechnology. Appl. Microbiol. Biotechnol. 2010, 86, 41-49.
2. Brecht, R., Disposable Bioreactors: Maturation into Pharmaceutical Glycoprotein Manufacturing. In Eibl, R., Eibl D. (Eds.), Disposable Bioreactors. Springer Series Advances in Biochemical Engineering/Biotechnology, Vol. 115, Springer, Berlin 2009, pp. 1-31.
3. Rao, G., Moreira, A., Brorson, K., Disposable bioprocessing: the future has arrived. Biotechnol. Bioeng. 2009, 102, 348-356.
4. DeWilde, D., Noack, U., Kahlert, W., Barbaroux, M., Greller, G., Bridging the gap from reusable to single-use manufacturing with stirred, single-use bioreactors. BioProcess Int. 2009, 7, 36-41.
5. Eibl, R., Werner, S., Eibl, D., Disposable bioreactors for plant liquid cultures at L-scale: review. Eng. Life Sci. 2009, 9, 156-164.
6. Eibl, R., Eibl, D., Application of Disposable Bag Bioreactors in Tissue Engineering and for the Production of Therapeutic Agents, Kasper, G., van Griensven, M., Pörtner, R. (Eds.), Bioreactor Systems for Tissue Engineering. Springer Series Advances in Biochemical Engineering/Biotechnology, Vol. 112, Springer, Berlin 2009, pp. 183-207.
7. Löffelholz, C., Kaiser, S. C., Werner, S., Eibl, D., CFD as a Tool to Characterize Single-use Bioreactors, Eibl, R., Eibl, D. (Eds.), Single-use Technology in Biopharmaceutical Manufacture, Wiley, NJ 2011.
8. User's Manual to FLUENT 6.3, Centerra Resource Park, 10 Cavendish Court, Lebanon, USA, Fluent Inc., 2006.
9. Paschedag A. R., CFD in der Verfahrenstechnik allgemeine Grundlagen und mehrphasige Anwendungen, Wiley, Weinheim 2004.
10. Launder, B., Spalding, D., The numerical computation of turbulent flows. Comp. Meth. Appl. Mech. Eng. 1974, 3, 269-289.
11. Armenante, P., Chou, C.-C., Velocity profiles in a baffled vessel with single or double pitched-blade turbines. AIChE J. 1996, 42, 42-54.
12. Ishii, M., Zuber, N., Drag coefficient and relative velocity in bubbly, droplet or particulate flows. AIChE J. 1979, 25, 843-855.
13. Kerdouss, F., Bannari, A., Proulx, P., Bannari, R. et al., Two-phase mass transfer coefficient prediction in stirred vessel with a CFD model. Comp. Chem. Eng. 2008, 32, 1943-1955.
14. Rizk, M. A., Elgobashi, S. E., A two-equation turbulence model for dispersed dilute confined two-phase flows. Int. J. Multiphase Flow 1989, 15, 119-133.
15. Naude, I., Xuereb, C., Bewand, J., Direct prediction of the flows induced by a propeller in an agitated vessel using an unstructured mesh. Can. J. Chem. Eng. 1998, 76, 631-640.
16. Lu, Z. Y., Liao, Y., Qian, D. Y., Large eddy simulations of a stirred tank using the Lattice Boltzmann method on a nonuniform grid. J. Comput. Phys. 2002, 181, 675-704.
17. Hiraoka, S., Kato, Y., Tada, Y., Ozaki, N. et al., Power consumption and mixing time in an agitated vessel with double impeller. Chem. Eng. Res. Des. 2001, 79, 805-810.
18. Schumpe, A., Adler, I., Deckwer, W.-D., Solubility of oxygen in electrolyte solutions. Biotechnol. Bioeng. 1978, 20, 145-150.
19. Hockey, R. M., Nouri, J., Turbulent flow in a baffled vessel stirred by a 60° pitched blade impeller. Chem. Eng. Sci. 1996, 51, 4405-4421.
20. Xing, Z., Kenty, B. M., Li, Z. J., Lee, S. S., Scale-up analysis for a CHO cell culture process in large-scale bioreactors. Biotechnol. Bioeng. 2009, 103, 733-746.
21. Lane, G. L., Koh, P. T. L., CFD Simulation of a Rushton turbine in a baffled tank, International Conference on CFD in Mineral and Metal Processing and Power Generation CSIRO 1997, 377-385.
22. Patwardhan, A., Prediction of flow characteristics and energy balance for a variety of downflow impellers. Ind. Eng. Chem. Res. 2001, 40, 3806-3816.
23. Liepe, F., Sperling, R., Jembere, S., Rührwerke - Theoretische Grundlagen, Auslegung und Bewertung. Eigenverlag FH Anhalt, Köthen 1998.
24. Nienow, A. W., On impeller circulation and mixing effectiveness in the turbulent flow regime. Chem. Eng. Sci. 1997, 52, 2557-2565.
25. Jaworski, Z., Bujalski, W., Otomo, N., Nienow, A., CFD study of homogenization with dual rushton turbines - comparison with experimental results, Part I: initial studies. Chem. Eng. Res. Des. 2000, 78, 327-333.
26. Min, J., Gao, Z., Large eddy simulations of mixing time in a stirred tank. Chin. J. Chem. Eng. 2006, 14, 1-7.
27. Zadghaffari, R., Moghaddas, J., Revstedt, J., A mixing study in a double-Rushton stirred tank. Comp. Chem. Eng. 2009, 33, 1240-1246.
28. Jahoda, M., Mostek, M., Kukuková, A., Machon, V., CFD modelling of liquid homogenization in stirred tanks with one and two impellers using large eddy simulation. Chem. Eng. Res. Des. 2007, 85, 616-625.
29. Van't Riet, K., Review of measuring methods and results in nonviscous gas-liquid mass transfer in stirred vessels. Ind. Eng. Chem. Process Des. Dev. 1979, 18, 357-364.
30. Henzler, H.-J., Kauling, D. J., Oxygenation of cell cultures. Bioprocess Biosys. Eng. 1993, 9, 61-75.
31. Langheinrich, C., Nienow, A. W., Eddleston, T., Stevenson, N. C. et al., Oxygen transfer in stirred bioreactors under animal cell culture conditions. Food Bioprod. Process. 2002, 80, 39-44.
32. Zhang, S., Handa-Corrigan, A., Spier, R. E., Oxygen transfer properties of bubbles in animal cell culture media. Biotechnol. Bioeng. 1992, 40, 252-259.
33. Nehring, D., Czermak, P., Vorlop, J., Lübben, H., Experimental study of a ceramic microsparging aeration system in a pilot-scale animal cell culture. Biotechnol. Prog. 2004, 20, 1710-1717.
34. Nienow, A. W., Reactor engineering in large scale animal cell culture. Cytotechnology 2006, 50, 9-33.
35. Chisti, Y., Animal-cell damage in sparged bioreactors. Trends Biotechnol. 2000, 18, 420-432.
36. Linek, V., Vacek, V., Benes, P., A critical review and experimental verification of the correct use of the dynamic method for the determination of oxygen transfer in aerated agitated vessels to water, electrolyte solutions and viscous liquids. Chem. Eng. J. 1987, 34, 11-34.
37. Gimbun, J., Rielly, C., Nagy, Z., Modelling of mass transfer in gas-liquid stirred tanks agitated by Rushton turbine and CD-6 impeller: a scale-up study. Chem. Eng. Res. Des. 2009, 87, 437-451.
38. Alves, S., Vasconcelos, J., Orvalho, S., Mass transfer to clean bubbles at low turbulent energy dissipation. Chem. Eng. Sci. 2006, 61, 1334-1337.
39. Kerdouss, F., Bannari, A., Proulx, P., CFD modeling of gas dispersion and bubble size in a double turbine stirred tank. Chem. Eng. Sci. 2006, 61, 3313-3322.
40. Laakkonen, M., Alopaeus, V., Aittamassa, J., Validation of bubble breakage, coalescence and mass transfer models for gas-liquid dispersion in agitated vessel. Chem. Eng. Sci. 2006, 61, 218-228.
41. Lane, G. L., Schwarz, M. P., Evans, G. M., Numerical modeling of gas-liquid flow in stirred tank. Chem. Eng. Sci. 2005, 60, 2203-2214.
42. Dhanasekharan, K., Sanyal, J., Jain, A., Haidari, A., A generalized approach to model oxygen transfer in bioreactors using population balances and computational fluid dynamics. Chem. Eng. Sci. 2005, 60, 213-218.
43. Zhang, H., Zhang, K., Fan, S., CFD simulation coupled with population balance equations for aerated stirred bioreactors. Eng. Life Sci. 2009, 9, 421-430.