The first performance report for the Bio-Rad Dx CT/NG/MG assay for simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in urogenital samples

Journal of Microbiological Methods

Volumn 89, Issue 3, 2012, Pages 193-197

  Le Roy, Chloé ^a,b   Le Hen, Isabelle ^d   Clerc, Maïthé ^a,b   Arfel, Véronique ^d   Normandin, Françoise ^d   Bébéar, Cécile ^a,b,c   de Barbeyrac, Bertille ^a,b,c  

^a UNIVERSITÉ DE BORDEAUX   (France)

^b Univ Victor Segalen Bordeaux 2   (France)

^c BORDEAUX UNIVERSITY HOSPITAL   (France)

^d Maison Départementale de la Santé   (France)

Author keywords

C. trachomatis; M. genitalium; N. gonorrhoeae; Real time PCR

Indexed keywords

ARTICLE; ASYMPTOMATIC INFECTION; BACTERIUM DETECTION; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DNA DETERMINATION; FALSE POSITIVE RESULT; FEMALE; GONORRHEA; HUMAN; INTERMETHOD COMPARISON; MALE; MYCOPLASMA GENITALIUM; MYCOPLASMOSIS; NEISSERIA GONORRHOEAE; NONHUMAN; NUCLEIC ACID AMPLIFICATION; PREDICTIVE VALUE; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UROGENITAL TRACT INFECTION;

ADOLESCENT; ADULT; BACTERIOLOGICAL TECHNIQUES; CHLAMYDIA TRACHOMATIS; FEMALE; FRANCE; GENITALIA, FEMALE; GENITALIA, MALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOPLASMA GENITALIUM; NEISSERIA GONORRHOEAE; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; SEXUALLY TRANSMITTED DISEASES, BACTERIAL; URINE; YOUNG ADULT;

CHLAMYDIA TRACHOMATIS; MYCOPLASMA GENITALIUM; NEISSERIA GONORRHOEAE;

EID: 84860360603     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.03.009     Document Type: Article

References (29)

1. Alloba F., Alawattegama A., Jones C. Use of nucleic acid amplification tests for the detection of Neisseria gonorrhoeae: survey of genitourinary medicine clinics in England. Int. J. STD AIDS 2007, 18:652-653.
2. Bébéar C., de Barbeyrac B. Genital Chlamydia trachomatis infections. Clin. Microbiol. Infect. 2009, 15:4-10.
3. Falk L., Fredlund H., Jensen J.S. Tetracycline treatment does not eradicate Mycoplasma genitalium. Sex. Transm. Infect. 2003, 79:318-319.
4. Falk L., Fredlund H., Jensen J.S. Signs and symptoms of urethritis and cervicitis among women with or without Mycoplasma genitalium or Chlamydia trachomatis infection. Sex. Transm. Infect. 2005, 81:73-78.
5. Gaydos C.A., Cartwright C.P., Colaninno P., Welsch J., Holden J., Ho S.Y., Webb E.M., Anderson C., Bertuzis R., Zhang L., Miller T., Leckie G., Abravaya K., Robinson J. Performance of the Abbott RealTime CT/NG for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 2010, 48:3236-3243.
6. Haggerty C.L. Evidence for a role of Mycoplasma genitalium in pelvic inflammatory disease. Curr. Opin. Infect. Dis. 2008, 21:65-69.
7. Hjorth S.V., Bjornelius E., Lidbrink P., Falk L., Dohn B., Berthelsen L., Ma L., Martin D.H., Jensen J.S. Sequence-based typing of Mycoplasma genitalium reveals sexual transmission. J. Clin. Microbiol. 2006, 44:2078-2083.
8. Hopkins M.J., Ashton L.J., Alloba F., Alawattegama A., Hart I.J. Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine. Sex. Transm. Infect. 2010, 86:207-211.
9. Jensen J.S., Bjornelius E., Dohn B., Lidbrink P. Use of TaqMan 5' nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J. Clin. Microbiol. 2004, 42:683-692.
10. Jensen J.S., Bjornelius E., Dohn B., Lidbrink P. Comparison of first void urine and urogenital swab specimens for detection of Mycoplasma genitalium and Chlamydia trachomatis by polymerase chain reaction in patients attending a sexually transmitted disease clinic. Sex. Transm. Dis. 2004, 31:499-507.
11. Kerndt P.R., Ferrero D.V., Aynalem G., Monga D., Wang S., Zhang N., Wong C., Liggins M., Meng Q. First report of the performance of the VERSANT(R) CT/GC DNA 1.0 assay (kPCR) for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 2011, 49:1347-1353.
12. Lee, S.J., Park, D.C., Lee, D.S., Choe, H.S. and Cho, Y.H., in press. Evaluation of Seeplex((R)) STD6 ACE detection kit for the diagnosis of six bacterial sexually transmitted infections. J. Infect. Chemother. doi:. doi:10-1007/S10156-011-0362-07.
13. Levett P.N., Brandt K., Olenius K., Brown C., Montgomery K., Horsman G.B. Evaluation of three automated nucleic acid amplification systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine specimens. J. Clin. Microbiol. 2008, 46:2109-2111.
14. Manavi K. A review on infection with Chlamydia trachomatis. Best Pract. Res. Clin. Obstet. Gynaecol. 2006, 20:941-951.
15. Manhart L.E., Holmes K.K., Hughes J.P., Houston L.S., Totten P.A. Mycoplasma genitalium among young adults in the United States: an emerging sexually transmitted infection. Am. J. Public Health 2007, 97:1118-1125.
16. Manhart L.E., Broad J.M., Golden M.R. Mycoplasma genitalium: should we treat and how ?. Clin. Infect. Dis. 2011, 53(Suppl. 3):S129-S142.
17. Mena L.A., Mroczkowski T.F., Nsuami M., Martin D.H. A randomized comparison of azithromycin and doxycycline for the treatment of Mycoplasma genitalium-positive urethritis in men. Clin. Infect. Dis. 2009, 48:1649-1654.
18. Ness R.B., Soper D.E., Holley R.L., Peipert J., Randall H., Sweet R.L., Sondheimer S.J., Hendrix S.L., Amortegui A., Trucco G., Songer T., Lave J.R., Hillier S.L., Bass D.C., Kelsey S.F. Effectiveness of inpatient and outpatient treatment strategies for women with pelvic inflammatory disease: results from the Pelvic Inflammatory Disease Evaluation and Clinical Health (PEACH) Randomized Trial. Am. J. Obstet. Gynecol. 2002, 186:929-937.
19. Oakeshott P., Aghaizu A., Hay P., Reid F., Kerry S., Atherton H., Simms I., Taylor-Robinson D., Dohn B., Jensen J.S. Is Mycoplasma genitalium in women the "New Chlamydia?" A community-based prospective cohort study. Clin. Infect. Dis. 2010, 51:1160-1166.
20. Peipert J.F. Clinical practice. Genital chlamydial infections. N. Engl. J. Med. 2003, 349:2424-2430.
21. Rockett R., Goire N., Limnios A., Turra M., Higgens G., Lambert S.B., Bletchly C., Nissen M.D., Sloots T.P., Whiley D.M. Evaluation of the cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae. Sex. Transm. Infect. 2010, 86:470-473.
22. Ross J.D., Brown L., Saunders P., Alexander S. Mycoplasma genitalium in asymptomatic patients: implications for screening. Sex. Transm. Infect. 2009, 85:436-437.
23. Schwebke J.R., Rompalo A., Taylor S., Sena A.C., Martin D.H., Lopez L.M., Lensing S., Lee J.Y. Re-evaluating the treatment of nongonococcal urethritis: emphasizing emerging pathogens-a randomized clinical trial. Clin. Infect. Dis. 2011, 52:163-170.
24. Semeniuk H., Zentner A., Read R., Church D. Evaluation of sequential testing strategies using non-amplified and amplified methods for detection of Chlamydia trachomatis in endocervical and urine specimens from women. Diagn. Microbiol. Infect. Dis. 2002, 42:43-51.
25. Shipitsyna E., Savicheva A., Solokovskiy E., Ballard R.C., Domeika M., Unemo M., Jensen J.S. Guidelines for the laboratory diagnosis of Mycoplasma genitalium infections in East European countries. Acta Derm. Venereol. 2010, 90:461-467.
26. Simel D.L., Samsa G.P., Matchar D.B. Likelihood ratios with confidence: sample size estimation for diagnostic test studies. J. Clin. Epidemiol. 1991, 44:763-770.
27. Skidmore S., Horner P., Herring A., Sell J., Paul I., Thomas J., Caul E.O., Egger M., McCarthy A., Sanford E., Salisbury C., Macleod J., Sterne J.A., Low N. Vulvovaginal-swab or first-catch urine specimen to detect Chlamydia trachomatis in women in a community setting?. J. Clin. Microbiol. 2006, 44:4389-4394.
28. Taylor-Robinson D., Jensen J.S. Mycoplasma genitalium: from Chrysalis to multicolored butterfly. Clin. Microbiol. Rev. 2011, 24:498-514.
29. Walker J., Fairley C.K., Bradshaw C.S., Tabrizi S.N., Chen M.Y., Twin J., Taylor N., Donovan B., Kaldor J.K., McNamee K., Urban E., Walker S., Currie M., Birden H., Bowden F., Gunn J., Pirotta M., Gurrin L., Harindra V., Garland S., Hocking J.S. The difference in determinants of Chlamydia trachomatis and Mycoplasma genitalium in a sample of young Australian women. BMC Infect. Dis. 2011, 11:35.