In situ assay of fatty acid β-oxidation by metabolite profiling following permeabilization of cell membranes

Journal of Lipid Research

Volumn 53, Issue 5, 2012, Pages 1012-1020

  Ensenauer, Regina ^a   Fingerhut, Ralph ^b,d   Schriever, Sonja C ^a   Fink, Barbara ^a   Becker, Marc ^a   Sellerer, Nina C ^a   Pagel, Philipp ^c   Kirschner, Andreas ^c   Dame, Torsten ^b   Olgemöller, Bernhard ^b   Röschinger, Wulf ^b   Roscher, Adelbert A ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b Becker and Colleagues Laboratory   (Germany)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^d UNIVERSITY CHILDREN S HOSPITAL   (Switzerland)

Author keywords

oxidation function; Acylcarnitines; Digitonin; Drug discovery; Fatty acid oxidation; Insulin resistance; Mitochondria; Mode of action; Obesity; Tandem mass spectrometry

Indexed keywords

ACYLCARNITINE; BEZAFIBRATE; CITRATE SYNTHASE; DIGITONIN; ETOMOXIR;

ARTICLE; CELL ISOLATION; CELL MEMBRANE PERMEABILITY; CHILD; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; FATTY ACID ANALYSIS; FATTY ACID OXIDATION; FIBROBLAST; HUMAN; HUMAN CELL; INCUBATION TIME; INTERMETHOD COMPARISON; MITOCHONDRIAL MEMBRANE; NORMAL HUMAN; PRIMARY CELL CULTURE; PRIORITY JOURNAL; PROCESS MONITORING; PROTEIN ISOLATION; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SOLID PHASE EXTRACTION; WHOLE CELL;

CELL LINE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CHILD; FATTY ACIDS; FIBROBLASTS; HUMANS; INFANT, NEWBORN; METABOLOMICS; MITOCHONDRIA; OXIDATION-REDUCTION; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TIME FACTORS;

EID: 84860299763     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D022608     Document Type: Article

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