Humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A

Veterinary Parasitology

Volumn 186, Issue 3-4, 2012, Pages 216-221

  Cunha, Ivo Alexandre Leme da ^a   Zulpo, Dauton Luiz ^a   Bogado, Alexey Leon Gomel ^a   de Barros, Luiz Daniel ^a   Taroda, Alessandra ^a   Igarashi, Michelle ^b   Navarro, Italmar Teodorico ^a   Garcia, João Luis ^a  

^a STATE UNIVERSITY OF LONDRINA   (Brazil)

^b UNIVERSITY OF CUIABÁ   (Brazil)

Author keywords

Mucosal immunity; Pigs; Rhoptry; Toxoplasma gondii

Indexed keywords

IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; PROTOZOAL PROTEIN; QUIL A; RHOPTRY PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; CELLULAR IMMUNITY; CONTROLLED STUDY; DRUG EFFECT; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMORAL IMMUNITY; IMMUNOGLOBULIN BLOOD LEVEL; IMMUNOSTIMULATION; INFECTION PREVENTION; LYMPHOCYTE PROLIFERATION; MALE; MOUSE; NONHUMAN; SWINE; TOXOPLASMA GONDII; TOXOPLASMOSIS;

ADJUVANTS, IMMUNOLOGIC; ADMINISTRATION, INTRANASAL; ANIMALS; BRAIN; CELL PROLIFERATION; IMMUNITY, CELLULAR; IMMUNITY, HUMORAL; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; LYMPHOCYTES; MICE; PROTOZOAN PROTEINS; PROTOZOAN VACCINES; SAPONINS; SWINE; SWINE DISEASES; TOXOPLASMA; TOXOPLASMOSIS, ANIMAL;

SUIDAE; TOXOPLASMA GONDII;

EID: 84860229316     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2011.11.034     Document Type: Article

References (28)

1. Camargo M.E. Introdução às técnicas de imunofluorescência. Rev. Bras. Patol. Clin. 1974, 10:143-171.
2. Chardés T., Bout D. Mucosal immune response in toxoplasmosis. Res. Immunol. 1993, 144:57-60.
3. Cox J.C., Coulter A.R. Adjuvants-a classification and review of their modes of action. Vaccine 1997, 15:246-256.
4. Dawson H.D., Beshah E., Nishi S., Solano-Aguilar G., Morimoto M., Zhao A., Madden K.B., Ledbetter T.K., Dubey J.P., Shea-Donohue T., Lunney J.K., Urban J.F. Localized multigene expression patterns support an evolving th1/th2-like paradigm in response to infections with Toxoplasma gondii and Ascaris suum. Infect. Immun. 2005, 73:1116-1128.
5. Dubey J.P., Frenkel J.K. Cyst-induced toxoplasmosis in cats. J. Protozool. 1972, 19:155-177.
6. Dubey J.P., Urban J.F., Davis S.W. Protective immunity to toxoplasmosis in pigs vaccinated with nonpersistent strain of Toxoplasma gondii. Am. J. Vet. Res. 1991, 52:1316-1319.
7. Dubey J.P., Baker D.G., Davis S.W., Urban J.F., Shen S.K. Persistence of immunity to toxoplasmosis in pigs vaccinated with a nonpersitent strain of Toxoplasma gondii. Am. J. Vet. Res. 1994, 55:982-987.
8. Dubey J.P. Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues. Vet. Parasitol. 1998, 74:75-77.
9. Dubey J.P., Lunney J.K., Shen S.K., Kwok O.C.H. Immunity to toxoplasmosis in pigs fed irradiated Toxoplasma gondii oocysts. J. Parasitol. 1998, 84:749-752.
10. Dubey J.P. Toxoplasmosis in pigs-the last 20 years. Vet. Parasitol. 2009, 164:89-103.
11. Freire R.L., Navarro I.T., Bracarense A.P.F.R.L. Vaccination of pigs with Toxoplasma gondii antigens incorporated in immunostimulating complexes (iscoms). Arq. Bras. Med. Vet. Zoot. 2003, 55:388-396.
12. Frenkel J.K., Pfefferkorn E.R., Smith D.D., Fishback J.L. Prospective vaccine prepared from a new mutant of Toxoplasma gondii for use in cats. Am. J. Vet. Res. 1991, 52:759-763.
13. Frenkel J.K., Smith D.D. Immunization of cats against shedding of Toxoplasma oocysts. J. Parasitol. 1982, 38:744-748.
14. Garcia J.L., Gennari S.M., Navarro I.T., Machado R.Z., Sinhorini I.L. Toxoplasma gondii: isolation of tachyzoites rhoptries and incorporation into Iscom. Exp. Parasitol. 2004, 108:40-46.
15. Garcia J.L., Gennari S.M., Navarro I.T., Machado R.Z., Sinhorini I.L., Freire R.L., Marana E.R., Tsutsui V., Contente A.P., Begale L.P. Partial protection against tissue cysts formation in pigs vaccinated with crude rhoptry proteins of Toxoplasma gondii. Vet. Parasitol. 2005, 129:209-217.
16. Garcia J.L., Navarro I.T., Vidotto O., Gennari S.M., Machado R.Z., da Luz Pereira A.B., Sinhorini I.L. Toxoplasma gondii: comparison of a rhoptry-ELISA with IFAT and MAT for antibody detection in sera of experimentally infected pigs. Exp. Parasitol. 2006, 113:100-105.
17. Garcia J.L. Vaccination concepts against Toxoplasma gondii. Exp. Rev. Vaccines 2009, 6:215-225.
18. Jongert V., Melkebeek S., De Craeye J., Dewit J., Verhelst D., Cox E. An enhanced Gra1-Gra7 cocktail DNA vaccine primes anti-Toxoplasma immune responses in pigs. Vaccine 2008, 26:1025-1031.
19. Jungersen G., Jensen L., Riber U., Heegaard P.M., Petersen E., Poulsen J.S., Bille-Hansen V., Lind P. Pathogenicity of selected Toxoplasma gondii isolates in young pigs. Int. J. Parasitol. 1999, 29:1307-1319.
20. Kashala O., Amador R., Valero M.V., Moreno A., Barbosa A., Nickel B., Daubenberger C.A., Guzman F., Pluschke G., Patarroyo M.E. Safety, tolerability and immunogenicity of new formulations of the Plasmodium falciparum malaria peptide vaccine SPf66 combined with the immunological adjuvant QS-21. Vaccine 2002, 20:2263-2277.
21. Neumann E.J., Grinberg A., Bonistalli K.N., Mack H.J., Lehrbach P.R., Gibson N. Safety of a live attenuated Erysipelothrix rhusiopathiae vaccine for swine. Vet. Microbiol. 2009, 135:297-303.
22. Siev D. Estimating vaccine efficacy in prospective studies. Prev. Vet. Med. 1994, 20:279-296.
23. Smith R.E., Donachie A.M., Mowat A.M. Immune stimulating complexes as mucosal vaccines. Immunol. Cell Biol. 1998, 76:263-269.
24. Solano-Aguilar G.I., Vengroski K.G., Beshah E., Vengroski K.G., Lunney J.K. Isolation and purification of lymphocyte subsets from gut-associated lymphoid tissue in neonatal swine. J. Immunol. Methods 2000, 241:185-199.
25. Solano Aguilar G.I., Beshah E., Vengroski K.G., Zarlenga D., Jauregui L., Cosio M., Douglass L.W., Dubey J.P., Lunney J.K. Cytokine and lymphocyte profiles in miniature swine after oral infection with Toxoplasma gondii oocysts. Int. J. Parasitol. 2001, 31:187-195.
26. Supply P., Sutton P., Coughlan S.N., Bilo K., Saman E., Trees A.J., Cesbraun-Delauw M.F., Locht C. Immunogenicity of recombinant BCG producing the GRA1 antigen from Toxoplasma gondii. Vaccine 1999, 17:705-714.
27. Yokomizo Y., Watanabe F., Imada Y., Inumaru S., Yanaka T., Tsuji T. Mucosal immunoadjuvant activity of the log toxic recombinant Escherichia coli heat-labile enterotoxin produced by Bacillus brevis for the bacterial subunit or component vaccine in pigs and cattle. Vet. Immunol. Immunopathol. 2002, 87:291-300.
28. Zhang L., Tian X., Zhou F. Intranasal administration of CpG oligonucleotides induces mucosaland systemic Type 1 immune responses and adjuvant activity to porcine reproductive and respiratory syndrome killed virus vaccine in piglets in vivo. Int. Immunopharmacol. 2007, 7:1732-1740.