Non-specific binding of antibodies in immunohistochemistry: Fallacies and facts

Scientific Reports

Volumn 1, Issue , 2011, Pages

  Buchwalow, Igor ^a   Samoilova, Vera ^a   Boecker, Werner ^a   Tiemann, Markus ^a  

^a Institute for Hematopathology   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; FC RECEPTOR;

ANIMAL; ANTIBODY COMBINING SITE; ARTICLE; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; MOUSE; RABBIT; RAT;

ANIMALS; ANTIBODIES; BINDING SITES, ANTIBODY; IMMUNOHISTOCHEMISTRY; MICE; RABBITS; RATS; RECEPTORS, FC;

EID: 84860140893     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep00028     Document Type: Article

References (23)

1. Ravetch, J. V. & Kinet, J. P. Fc Receptors. Annu. Rev. Immunol. 9, 457-492 (1991).
2. Cruse, J. M. & Lewis, R. E. Atlas of Immunology (Springer, Heidelberg, 2000).
3. Zhu, X. et al. MHC class I-related neonatal Fc receptor for IgG is functionally expressed in monocytes, intestinal macrophages, and dendritic cells. J. Immunol. 166, 3266-3276 (2001). (Pubitemid 33117326)
4. Bussolati, G. & Leonardo, E. Technical pitfalls potentially affecting diagnoses in immunohistochemistry. J. Clin. Pathol. 61, 1184-1192 (2008).
5. Daneshtalab, N., Dore, J. J. & Smeda, J. S. Troubleshooting tissue specificity and antibody selection: Procedures in immunohistochemical studies. J. Pharmacol. Toxicol. Methods 61, 127-135 (2010).
6. Fritschy, J. M. Is my antibody-staining specific? How to deal with pitfalls of immunohistochemistry. Eur. J. Neurosci. 28, 2365-2370 (2008).
7. Ramos-Vara, J. A. Technical aspects of immunohistochemistry. Vet. Pathol. 42, 405-426 (2005). (Pubitemid 40993852)
8. Alexander, E. L. & Sanders, S. K. F(ab9)2 reagents are not required if goat, rather than rabbit, antibodies are used to detect human surface immunoglobulin. J. Immunol. 119, 1084-1088 (1977). (Pubitemid 8202978)
9. Boenisch, T. Handbook on Immunohistochemical Staining Methods (DAKO Corporation, Carpinteria, , CA., 2001).
10. Lu, Q. L. & Partridge, T. A. A new blocking method for application of murine monoclonal antibody to mouse tissue sections. J. Histochem. Cytochem. 46, 977-984 (1998).
11. Brandon, C. Improved immunocytochemical staining through the use of Fab fragments of primary antibody, Fab-specific second antibody, and Fabhorseradish peroxidase. J. Histochem. Cytochem. 33, 715-719 (1985). (Pubitemid 15049530)
12. Kim, S. H. et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining. J. Histochem. Cytochem. 51, 129-132 (2003). (Pubitemid 36133495)
13. Renshaw, S. Immunohistochemistry (Scion Publishing Ltd, Cambridge, 2007).
14. Hurley, I. P., Coleman, R. C., Ireland, H. E., & Williams, J. H. Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration. J. Dairy Sci. 87, 543-549 (2004). (Pubitemid 44151741)
15. Pino, R. M. Binding of Fab-horseradish peroxidase conjugates by charge and not by immunospecificity. J. Histochem. Cytochem. 33, 55-58 (1985). (Pubitemid 15189492)
16. Juhl, B. R., Norgaard, T. & Bjerrum, O. J. The effect of Tween 20 on indirect immunoperoxidase staining of blood group antigen A in human urothelium. J. Histochem. Cytochem. 32, 935-941 (1984). (Pubitemid 14054999)
17. Richards, J. M. & Witkin, S. S. Non-immune IgG binding to the surface of spermatozoa by disulphide rearrangement. Clin. Exp. Immunol. 58, 493-501 (1984). (Pubitemid 15201873)
18. Rogers, A. B., Cormier, K. S. & Fox, J. G. Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistry. Lab. Invest. 86, 526-533 (2006).
19. Elias, J. M. Immunohistochemical methods in Immunohistopathology. in A Practical Approach to Diagnosis (ed. Elias, J. M. ) 1-110 (ASCP Press, Chicago, IL, 2003).
20. Larsson, L. I. Immunocytochemistry: Theory and Practice (CRC Press, Boca Raton, , FL, 1988).
21. Weston, P. D. & Poole, A. R. Antibodies to enzymes and their use, with specific reference to cathepsin D and other lysosomal enzymes in Lysosomes in Biology and Pathology (ed. Dingle, J. T. ) 425-464 (North Holland Publishing, Amsterdam, 1973).
22. Miller, R. T. Technical Immunohistochemistry: Achieving Reliability and Reproducibility of Immunostains. Society for Applied Immunohistochemistry, 2001 Annual Meeting. 1-56 (2011). Full text available on the Website, http://www.ihcworld.com/-books/Miller-handout. pdf.
23. Buchwalow, I. B. & Boecker, W. Immunohistochemistry: Basics and Methods (Springer, Heidelberg, Dordrecht, London, New York, 2010).