Development and Validation of a Simple GC-MS Method for the Simultaneous Determination of 11 Anticholinesterase Pesticides in Blood-Clinical and Forensic Toxicology Applications

Journal of Forensic Sciences

Volumn 57, Issue 3, 2012, Pages 806-812

  Papoutsis, Ioannis ^a   Mendonis, Marcela ^a   Nikolaou, Panagiota ^a   Athanaselis, Sotirios ^a   Pistos, Constantinos ^a   Maravelias, Constantinos ^a   Spiliopoulou, Chara ^a  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

Author keywords

Anticholinesterase pesticides; Blood; Clinical and forensic toxicology; Forensic science; Gas chromatography mass spectrometry; Liquid liquid extraction

Indexed keywords

CHOLINESTERASE INHIBITOR; PESTICIDE; SOLVENT;

ADULT; ARTICLE; BLOOD; FORENSIC TOXICOLOGY; HUMAN; LIMIT OF DETECTION; MALE; MASS FRAGMENTOGRAPHY; METHODOLOGY; MIDDLE AGED; PRESCHOOL CHILD; VALIDATION STUDY;

ADULT; CHILD, PRESCHOOL; CHOLINESTERASE INHIBITORS; FORENSIC TOXICOLOGY; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; LIMIT OF DETECTION; MALE; MIDDLE AGED; PESTICIDES; SOLVENTS;

EID: 84860134406     PISSN: 00221198     EISSN: 15564029     Source Type: Journal    
DOI: 10.1111/j.1556-4029.2011.02031.x     Document Type: Article

References (39)

1. Lacassie E, Marquet P, Gaulier JM, Dreyfuss MF, Lachatre G. Sensitive and specific multiresidue methods for the determination of pesticides of various classes in clinical and forensic toxicology. Forensic Sci Int 2001;121:116-25.
2. Barr DB, Needham LL. Analytical methods for biological monitoring of exposure to pesticides: a review. J Chromatogr B 2002;778:5-29.
3. Olsson AO, Nguyen JV, Sadowski MA, Barr DB. A liquid chromatography/electrospray ionization-tandem mass spectrometry method for quantification of specific organophosphorus pesticide biomarkers in human urine. Anal Bioanal Chem 2003;376:808-15.
4. Barr DB, Barr JR, Maggio VL, Whitehead RD, Sadowski MA, Whyatt RM, et al. A multi-analyte method for the quantification of contemporary pesticides in human serum and plasma using high-resolution mass spectrometry. J Chromatogr B 2002;778:99-111.
5. Pitarch E, Serrano R, Lopez FZ, Hernandez F. Rapid multiresidue determination of organochlorine and organophosphorus compounds in human serum by solid-phase extraction and gas chromatography coupled to tandem mass spectrometry. Anal Bioanal Chem 2003;376:189-97.
6. Tutudaki M, Tsatsakis A. Pesticide hair analysis: development of a GC-NCI-MS method to assess chronic exposure to diazinon in rats. J Anal Toxicol 2005;29:805-9.
7. Tarbah FA, Kardel B, Pier S, Daldrup T. Acute poisoning with phosphamidon: determination of dimethyl phosphate (DMP) as a stable metabolite in a case of organophosphate insecticide intoxication. J Anal Toxicol 2004;28:198-203.
8. Sorensen FW, Gregersen M. Rapid lethal intoxication caused by the herbicide glyphospate-trimesium (Touchdown). Hum Exp Toxicol 1999;18:735-7.
9. Jamil H. Acute poisoning-a review of 1900 cases. J Pak Med Assoc 1990;40:131-3.
10. Casey P, Vale JA. Deaths from pesticides poisoning in England and Wales (1945-1989). Hum Exp Toxicol 1994;13:95-101.
11. Lifshitz M, Shahak E, Bolotin A, Sofer S. Carbamate and organophosphate poisoning in young children. Pediatr Emerg Care 1999;15(2):102-3.
12. Meyer E, Barrey D, Lambert W, Van Peteghem C, Pielte M, Leenheer A. Analysis of fenthion in postmortem samples by HPLC with diode-array detection and GC/MS using solid-phase extraction. J Anal Toxicol 1998;22:248-52.
13. Estela G, Marques P. Acute intoxication by azinphos-ethyl. J Anal Toxicol 1990;14:243-6.
14. Kintz P, Jamey C, Doray S, Ludes B. Acute fatal poisoning with dichlorophen. Int J Legal Med 1997;110:95-6.
15. Cocker J, Mason HJ, Garfitt SJ, Jones K. Biological monitoring of exposure to organophosphate pesticides. Toxicol Lett 2002;134:97-103.
16. Hernandez F, Sancho JV, Pozo OJ. An estimation of the exposure to organophosphorus pesticides through the simultaneous determination of their main metabolites in urine by liquid chromatography-tandem mass spectrometry. J Chromatogr B 2004;808:229-39.
17. Tarbah FA, Mahler H, Temme O, Daldrup T. An analytical method for the rapid screening of organophosphate pesticides in human biological samples and foodstuffs. Forensic Sci Int 2001;121:126-33.
18. Thompson TS, Treble RG, Magliocco A, Rottger JR. Case study: fatal poisoning by malathion. Forensic Sci Int 1998;95:89-98.
19. Liu S, Pleil JD. Human blood and environmental media screening method for pesticides and polychlorinated biphenyl compounds using liquid extraction and gas chromatography-mass spectrometry analysis. J Chromatogr B 2002;769:155-67.
20. Cho Y, Matsuoka N, Kamiya A. Determination of organophosphorus pesticides in biological samples of acute poisoning by HPLC with diode-array-detector. Chem Pharm Bull 1997;45(4):737-40.
21. Sharma VK, Jadhav RK, Rao GL, Saraf AK, Chandra H. High performance liquid chromatographic method for the analysis of organophosphorus and carbamates pesticides. Forensic Sci Int 1990;48:21-5.
22. Kojima T, Yashiki M, Ohtani M, Chikasue F, Miyazaki T. Determination of dimethoate in blood and hemoperfusion cartridge following ingestion of formothion: a case study. Forensic Sci Int 1990;48:79-88.
23. Musshoff F, Junker H, Madea B. Rapid analysis of parathion in biological samples using headspace solid-phase micro-extraction (HS-SPME) and gas chromatography/mass spectrometry. Clin Chem Lab Med 1999;37(6):639-42.
24. Vasilic Z, Bozena S, Drevenkar V. Dimethylphosphorus metabolites in serum and urine of persons poisoned by malathion or thiometon. Chem Biol Interactions 1999;119:479-87.
25. Possecion N, Ostrea E, Bielawski D, Corrion M, Seagraves J, Jin Y. Detection of exposure to environmental pesticides during pregnancy by the analysis of maternal hair using GC-MS. Chromatographia 2006;64(11-12):681-7.
26. Dmitrovic J, Chan SC, Chan SHY. Analysis of pesticides and PCB congeners in serum by GC/MS with SPE sample cleanup. Toxicol Lett 2002;134:253-8.
27. Frenzel T, Sochor H, Speer K, Uihleim M. Rapid multimethod for verification and determination of toxic pesticides in whole blood by means of capillary GC-MS. J Anal Toxicol 2000;24:365-71.
28. Medina CM, Pitarch E, Portlés T, López FJ, Hernández F. GC-MS/MS multi-residue method for the determination of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in human breast tissues. J Sep Sci 2009;32:2090-102.
29. Bravo R, Driskell WJ, Whitehead RD, Needham LL, Barr DB. Quantitation of dialkyl phosphate metabolites of organophosphate pesticides in human urine using GC-MS-MS with isotopic internal standards. J Anal Toxicol 2002;26:245-52.
30. Vidal JLM, Frías MM, Frenich AG, Olea-Serrano F, Olea N. Determination of endocrine-disrupting pesticides and polychlorinated biphenyls in human serum by GC-ECD and GC-MS-MS and evaluation of contributors to the uncertainty of the results. Anal Bioanal Chem 2002;372:766-75.
31. Oglobline AN, Elimelakh H, Tattam B, Geyer R, O'Donnell GE, Holder G. Negative ion chemical ionization GC/MS-MS analysis of dialkylphosphate metabolites of organophosphoshate pesticides in urine of non-occupationally exposed subjects. Analyst 2001;126(7):1037-41.
32. Kawasaki S, Ueda H, Itoh H, Tadano J. Screening of organophosphorus pesticides using liquid-chromatography/mass spectrometry. J Chromatogr 1992;595(1-2):193-202.
33. Itoh H, Kawasaki S, Tadano J. Application of liquid-chromatography/mass spectrometry to pesticide analysis. J Chromatogr A 1996;754:61-76.
34. Inoue S, Saito T, Mase H, Suzuki Y, Takazawa K, Yamamoto I, et al. Rapid simultaneous determination for organophosphorus pesticides in human serum by LC-MS. J Pharm Biomed Anal 2007;44:258-64.
35. John H, Eddleston M, Clutton RE, Worek F, Thiermann H. Simultaneous quantification of the organophosphorus pesticides dimethoate and omethoate in porcine plasma and urine by LC-ESI-MS/MS and flow-injection-ESI-MS/MS. J Chromatogr B 2010;878:1234-45.
36. Ramesh A, Ravi PE. Electron ionization gas chromatography-mass spectrometric determination of residues of thirteen pyrethroid insecticides in whole blood. J Chromatogr B 2004;802:371-6.
37. Food and Drug Administration. Guidance for industry: bioanalytical method validation. Rockville, MD: US Department of Health and Human Services, FDA, Center for Drug Evaluation and Research, 2001.
38. ICH Expert Working Group, International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use. ICH harmonized tripartite guideline. Validation of analytical procedures: text and methodology Q2 (R1). Step 4 version, 2005, (accessed December 27, 2010).
39. Ageda S, Fuke C, Ihama Y, Miyazaki T. The stability of organophosphorus insecticides in fresh blood. Leg Med 2006;8:144-9.