Highly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization

Molecular and Cellular Probes

Volumn 26, Issue 3, 2012, Pages 113-115

  Ma, Changbei ^a,b  

^a CENTRAL SOUTH UNIVERSITY   (China)

^b IOWA STATE UNIVERSITY   (United States)

Author keywords

Alkaline phosphatase; Inhibitor; Molecular beacon

Indexed keywords

ALKALINE PHOSPHATASE; OKADAIC ACID;

ARTICLE; FEASIBILITY STUDY; LIMIT OF DETECTION; MOLECULAR BEACON; PRIORITY JOURNAL; PROTEIN POLYMERIZATION;

ALKALINE PHOSPHATASE; MOLECULAR PROBES; OKADAIC ACID; POLYMERIZATION;

EID: 84859984717     PISSN: 08908508     EISSN: 10961194     Source Type: Journal    
DOI: 10.1016/j.mcp.2012.03.005     Document Type: Article

References (22)

1. Bronstein I., Martin C.S., Fortin J.J., Olesen C.E.M., Voyta J.C. Chemiluminescence: sensitive detection technology for reporter gene assays. Clin Chem 1996, 42:1542-1546.
2. Benez A., Geiselhart A., Handgretinger R., Schiebel U., Fierlbeck G. Detection of circulating melanoma cells by immunomagnetic cell sorting. J Clin Lab Anal 1999, 13:229-233.
3. Fishman W.H. Clinical and biological significance of an isozyme tumor marker-PLAP. Clin Biochem 1987, 20:387-392.
4. Ximenes V.F., Campa A., Baader W.J., Catalani L.H. Facile chemiluminescent method for alkaline phosphatase determination. Anal Chim Acta 1999, 402:99-104.
5. Kokado A., Arakawa H., Maeda M. Chemiluminescence assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin. Luminescence 2002, 17:5-10.
6. -14 mole. Biochem Biophys Res Commun 1962, 9:430-435.
7. Zhu X.J., Jiang C.Q. 8-Quinolyl phosphate as a substrate for the fluorimetric determination of alkaline phosphatase. Clinica Chim Acta 2007, 377:150-153.
8. Radi A.E., Montornes J.M., O'Sullivan C.K. Reagentless detection of alkaline phosphatase using electrochemically grafted films of aromatic diazonium salts. J Electroanal Chem 2006, 587:140-147.
9. Robert E.G., Andrea B., Geza N., Michael R.N., Erno L. Amperometric microcells for alkaline phosphatase assay. Analyst 2002, 127:235-240.
10. Serra B., Morales M.D., Reviejo A.J., Hall E.H., Pingarron J.M. Rapid and highly sensitively electrochemical determination of alkaline phosphatase using a composite tyrosinase biosensor. Anal Biochem 2005, 336:289-294.
11. Robert K., Dominika O., Stanislaw G. Potentiometric assay for acid and alkaline phosphatase. Anal Chim Acta 2005, 53:257-261.
12. Ruan C.M., Wang W., Gu B.H. Detection of alkaline phosphatase using surface-enhanced Raman spectroscopy. Anal Chem 2006, 78:3339-3384.
13. Tyagi S., Kramer F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotech 1996, 14:303-308.
14. Tyagi S., Bratu D.P., Kramer F.R. Multicolor molecular beacons for allele discrimination. Nat Biotech 1998, 16:49-53.
15. Tan W., Wang K., Drake T.J. Molecular beacons. Curr Opin Chem Biol 2004, 8:547-553.
16. Tang Z., Wang K., Tan W., Ma C., Li J., Liu L., et al. Real-time investigation of nucleic acids phosphorylation process using molecular beacon. Nucleic Acids Res 2005, 33:e97.
17. Tang Z., Wang K., Tan W., Li J., Liu L., Guo Q., et al. Real-time monitoring of nucleic acid ligation in homogenous solution using molecular beacon. Nucleic Acids Res 2003, 31:e148.
18. Ma C., Tang Z., Wang K., Tan W., Li J., Li W., et al. Real-time monitoring of DNA polymerase activity using molecular beacon. Anal Biochem 2006, 353:141-143.
19. Ma C., Tang Z., Wang K., Tan W., Yang X., Li W., et al. Real-time monitoring of restriction endonuclease activity using molecular beacon. Anal Biochem 2007, 363:294-296.
20. Limoges B., Degrand C. Ferrocenylethyl phosphate: an improved substrate for the detection of alkaline phosphatase by cathodic stripping ion-exchange voltammetry. Application to the electrochemical enzyme affinity assay of avidin. Anal Chem 1996, 68:4141-4148.
21. Mestrovic V., Pavela-Vrancic M. Inhibition of alkaline phosphatase activity by okadaic acid, a protein phosphatase inhibitor. Biochimie 2003, 85:647-650.
22. Fujiki H., Suganuma M. Tumor promotion by inhibitors of protein phosphatases 1 and 2A: the okadaic acid class of compounds. Adv Cancer Res 1993, 61:143-194.