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Volumn 18, Issue 5, 2012, Pages 1075-1090

In vitro reconstitution of yeast splicing with U4 snRNA reveals multiple roles for the 3′ stem-loop

Author keywords

Di snRNP formation; In vitro reconstitution; Pre mRNA splicing; Protein RNA interaction; Spliceosome activation; Splicing complementation; U4 snRNA

Indexed keywords

SMALL NUCLEAR RIBONUCLEOPROTEIN; SMALL NUCLEAR RNA;

EID: 84859952475     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.031757.111     Document Type: Article
Times cited : (6)

References (38)
  • 1
    • 0029146556 scopus 로고
    • SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing
    • Ansari A, Schwer B. 1995. SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing. EMBO J 14: 4001-4009.
    • (1995) EMBO J , vol.14 , pp. 4001-4009
    • Ansari, A.1    Schwer, B.2
  • 3
    • 0031016867 scopus 로고    scopus 로고
    • Mutations within the yeast U4/U6 snRNP protein Prp4 affect a late stage of spliceosome assembly
    • Ayadi L, Miller M, Banroques J. 1997. Mutations within the yeast U4/ U6 snRNP protein Prp4 affect a late stage of spliceosome assembly. RNA 3: 197-209. (Pubitemid 27074573)
    • (1997) RNA , vol.3 , Issue.2 , pp. 197-209
    • Ayadi, L.1    Miller, M.2    Banroques, J.3
  • 4
    • 0023058996 scopus 로고
    • U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation
    • Berget SM, Robberson BL. 1986. U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation. Cell 46: 691-696.
    • (1986) Cell , vol.46 , pp. 691-696
    • Berget, S.M.1    Robberson, B.L.2
  • 5
    • 0025336899 scopus 로고
    • Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo
    • Bordonné R, Banroques J, Abelson J, Guthrie C. 1990. Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo. Genes Dev 4: 1185-1196.
    • (1990) Genes Dev , vol.4 , pp. 1185-1196
    • Bordonné, R.1    Banroques, J.2    Abelson, J.3    Guthrie, C.4
  • 6
    • 33646173207 scopus 로고    scopus 로고
    • Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain
    • Brenner TJ, Guthrie C. 2006. Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. RNA 12: 862-871.
    • (2006) RNA , vol.12 , pp. 862-871
    • Brenner, T.J.1    Guthrie, C.2
  • 7
    • 0023782352 scopus 로고
    • Spliceosomal RNA U6 is remarkably conserved from yeast to mammals
    • Brow DA, Guthrie C. 1988. Spliceosomal RNA U6 is remarkably conserved from yeast to mammals. Nature 334: 213-218.
    • (1988) Nature , vol.334 , pp. 213-218
    • Brow, D.A.1    Guthrie, C.2
  • 8
    • 0023449728 scopus 로고
    • Spliceosome assembly in yeast
    • Cheng SC, Abelson J. 1987. Spliceosome assembly in yeast. Genes Dev 1: 1014-1027.
    • (1987) Genes Dev , vol.1 , pp. 1014-1027
    • Cheng, S.C.1    Abelson, J.2
  • 9
    • 0024792715 scopus 로고
    • In vitro assembly of yeast U6 snRNP: A functional assay
    • Fabrizio P, McPheeters DS, Abelson J. 1989. In vitro assembly of yeast U6 snRNP: a functional assay. Genes Dev 3: 2137-2150. (Pubitemid 20023238)
    • (1989) Genes and Development , vol.3 , Issue.12 B , pp. 2137-2150
    • Fabrizio, P.1    McPheeters, D.S.2    Abelson, J.3
  • 10
    • 0029285869 scopus 로고
    • Specificity of Prp24 binding to RNA: A role for Prp24 in the dynamic interaction of U4 and U6 snRNAs
    • Ghetti A, Company M, Abelson J. 1995. Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs. RNA 1: 132-145.
    • (1995) RNA , vol.1 , pp. 132-145
    • Ghetti, A.1    Company, M.2    Abelson, J.3
  • 13
    • 0027153257 scopus 로고
    • A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae
    • Horowitz DS, Abelson J. 1993. A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Mol Cell Biol 13: 2959-2970. (Pubitemid 23133967)
    • (1993) Molecular and Cellular Biology , vol.13 , Issue.5 , pp. 2959-2970
    • Horowitz, D.S.1    Abelson, J.2
  • 14
    • 0029447484 scopus 로고
    • The central region of the yeast U4 snRNA is not important for hammerhead catalysis, but may act as a domain spacer
    • Hu J, Field D, Wang A, Friesen JD. 1995a. The central region of the yeast U4 snRNA is not important for hammerhead catalysis, but may act as a domain spacer. Nucleic Acids Symp Ser 1995: 25-28.
    • (1995) Nucleic Acids Symp Ser , vol.1995 , pp. 25-28
    • Hu, J.1    Field, D.2    Wang, A.3    Friesen, J.D.4
  • 15
    • 0028881597 scopus 로고
    • Mutational analysis of Saccharomyces cerevisiae U4 small nuclear RNA identifies functionally important domains
    • Hu J, Xu D, Schappert K, Xu Y, Friesen JD. 1995b. Mutational analysis of Saccharomyces cerevisiae U4 small nuclear RNA identifies functionally important domains. Mol Cell Biol 15: 1274-1285.
    • (1995) Mol Cell Biol , vol.15 , pp. 1274-1285
    • Hu, J.1    Xu, D.2    Schappert, K.3    Xu, Y.4    Friesen, J.D.5
  • 16
    • 0023672447 scopus 로고
    • Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein
    • Lamond AI, Konarska MM, Grabowski PJ, Sharp PA. 1988. Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein. Proc Natl Acad Sci 85: 411-415.
    • (1988) Proc Natl Acad Sci , vol.85 , pp. 411-415
    • Lamond, A.I.1    Konarska, M.M.2    Grabowski, P.J.3    Sharp, P.A.4
  • 17
    • 79957601559 scopus 로고    scopus 로고
    • Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis
    • Leung AKW, Nagai K, Li J. 2011. Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis. Nature 473: 536-539.
    • (2011) Nature , vol.473 , pp. 536-539
    • Leung, A.K.W.1    Nagai, K.2    Li, J.3
  • 18
    • 33744937919 scopus 로고    scopus 로고
    • Exon ligation is proofread by the DExD/H-box ATPase Prp22p
    • DOI 10.1038/nsmb1093, PII N1093
    • Mayas RM, Maita H, Staley JP. 2006. Exon ligation is proofread by the DExD/H-box ATPase Prp22p. Nat Struct Mol Biol 13: 482-490. (Pubitemid 43848902)
    • (2006) Nature Structural and Molecular Biology , vol.13 , Issue.6 , pp. 482-490
    • Mayas, R.M.1    Maita, H.2    Staley, J.P.3
  • 19
    • 39449114521 scopus 로고    scopus 로고
    • The U1, U2 and U5 snRNAs crosslink to the 59 exon during yeast pre-mRNA splicing
    • DOI 10.1093/nar/gkm1098
    • McGrail JC, O'Keefe RT. 2008. The U1, U2 and U5 snRNAs crosslink to the 59 exon during yeast pre-mRNA splicing. Nucleic Acids Res 36: 814-825. (Pubitemid 351267238)
    • (2008) Nucleic Acids Research , vol.36 , Issue.3 , pp. 814-825
    • McGrail, J.C.1    O'Keefe, R.T.2
  • 20
    • 33748029810 scopus 로고    scopus 로고
    • Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing
    • DOI 10.1038/sj.emboj.7601258, PII 7601258
    • McGrail JC, Tatum EM, O'Keefe RT. 2006. Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing. EMBO J 25: 3813-3822. (Pubitemid 44300267)
    • (2006) EMBO Journal , vol.25 , Issue.16 , pp. 3813-3822
    • McGrail, J.C.1    Tatum, E.M.2    O'Keefe, R.T.3
  • 21
    • 0024788534 scopus 로고
    • In vitro reconstitution of functional yeast U2 snRNPs
    • McPheeters DS, Fabrizio P, Abelson J. 1989. In vitro reconstitution of functional yeast U2 snRNPs. Genes Dev 3: 2124-2136. (Pubitemid 20023237)
    • (1989) Genes and Development , vol.3 , Issue.12 B , pp. 2124-2136
    • McPheeters, D.S.1    Fabrizio, P.2    Abelson, J.3
  • 22
    • 0026013119 scopus 로고
    • A phylogenetic study of U4 snRNA reveals the existence of an evolutionarily conserved secondary structure corresponding to ''free'' U4 snRNA
    • Myslinski E, Branlant C. 1991. A phylogenetic study of U4 snRNA reveals the existence of an evolutionarily conserved secondary structure corresponding to ''free'' U4 snRNA. Biochimie 73: 17-28.
    • (1991) Biochimie , vol.73 , pp. 17-28
    • Myslinski, E.1    Branlant, C.2
  • 23
    • 0030598839 scopus 로고    scopus 로고
    • The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast
    • DOI 10.1016/S0092-8674(00)80140-3
    • O'Keefe RT, Norman C, Newman AJ. 1996. The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of premRNA splicing in yeast. Cell 86: 679-689. (Pubitemid 26299498)
    • (1996) Cell , vol.86 , Issue.4 , pp. 679-689
    • O'Keefe, R.T.1    Norman, C.2    Newman, A.J.3
  • 25
    • 0032488860 scopus 로고    scopus 로고
    • A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles
    • DOI 10.1126/science.279.5352.857
    • Raghunathan PL, Guthrie C. 1998a. A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles. Science 279: 857-860. (Pubitemid 28106278)
    • (1998) Science , vol.279 , Issue.5352 , pp. 857-860
    • Raghunathan, P.L.1    Guthrie, C.2
  • 26
    • 0032537739 scopus 로고    scopus 로고
    • RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
    • Raghunathan PL, Guthrie C. 1998b. RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr Biol 8: 847-855. (Pubitemid 28356043)
    • (1998) Current Biology , vol.8 , Issue.15 , pp. 847-855
    • Raghunathan, P.L.1    Guthrie, C.2
  • 27
    • 0029124901 scopus 로고
    • In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs
    • Ségault V, Will CL, Sproat BS, Lührmann R. 1995. In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs. EMBO J 14: 4010-4021.
    • (1995) EMBO J , vol.14 , pp. 4010-4021
    • Ségault, V.1    Will, C.L.2    Sproat, B.S.3    Lührmann, R.4
  • 28
    • 0036850385 scopus 로고    scopus 로고
    • Domains of human U4atac snRNA required for U12-dependent splicing in vivo
    • Shukla GC, Cole AJ, Dietrich RC, Padgett RA. 2002. Domains of human U4atac snRNA required for U12-dependent splicing in vivo. Nucleic Acids Res 30: 4650-4657. (Pubitemid 35331746)
    • (2002) Nucleic Acids Research , vol.30 , Issue.21 , pp. 4650-4657
    • Shukla, G.C.1    Cole, A.J.2    Dietrich, R.C.3    Padgett, R.A.4
  • 29
    • 0025007677 scopus 로고
    • Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes
    • Vankan P, McGuigan C, Mattaj IW. 1990. Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes. EMBO J 9: 3397-3404.
    • (1990) EMBO J , vol.9 , pp. 3397-3404
    • Vankan, P.1    McGuigan, C.2    Mattaj, I.W.3
  • 30
    • 0026574985 scopus 로고
    • Roles of U4 and U6 snRNAs in the assembly of splicing complexes
    • Vankan P, McGuigan C, Mattaj IW. 1992. Roles of U4 and U6 snRNAs in the assembly of splicing complexes. EMBO J 11: 335-343.
    • (1992) EMBO J , vol.11 , pp. 335-343
    • Vankan, P.1    McGuigan, C.2    Mattaj, I.W.3
  • 31
    • 0022747370 scopus 로고
    • Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome
    • Vijayraghavan U, Parker R, Tamm J, Iimura Y, Rossi J, Abelson J, Guthrie C. 1986. Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome. EMBO J 5: 1683-1695.
    • (1986) EMBO J , vol.5 , pp. 1683-1695
    • Vijayraghavan, U.1    Parker, R.2    Tamm, J.3    Iimura, Y.4    Rossi, J.5    Abelson, J.6    Guthrie, C.7
  • 32
    • 0025037427 scopus 로고
    • Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly
    • Wersig C, Bindereif A. 1990. Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly. Nucleic Acids Res 18: 6223-6229.
    • (1990) Nucleic Acids Res , vol.18 , pp. 6223-6229
    • Wersig, C.1    Bindereif, A.2
  • 33
    • 0026582386 scopus 로고
    • Reconstitution of functional mammalian U4 small nuclear ribonucleoprotein: Sm protein binding is not essential for splicing in vitro
    • Wersig C, Bindereif A. 1992. Reconstitution of functional mammalian U4 small nuclear ribonucleoprotein: Sm protein binding is not essential for splicing in vitro. Mol Cell Biol 12: 1460-1468.
    • (1992) Mol Cell Biol , vol.12 , pp. 1460-1468
    • Wersig, C.1    Bindereif, A.2
  • 34
    • 0026693309 scopus 로고
    • Assembly and nuclear transport of the U4 and U4/U6 snRNPs
    • Wersig C, Guddat U, Pieler T, Bindereif A. 1992. Assembly and nuclear transport of the U4 and U4/U6 snRNPs. Exp Cell Res 199: 373-377.
    • (1992) Exp Cell Res , vol.199 , pp. 373-377
    • Wersig, C.1    Guddat, U.2    Pieler, T.3    Bindereif, A.4
  • 35
    • 0029847221 scopus 로고    scopus 로고
    • In vitro reconstitution of mammalian U1 snRNPs active in splicing: The U1-C protein enhances the formation of early (E) spliceosomal complexes
    • Will CL, Rümpler S, Klein Gunnewiek J, van Venrooij WJ, Lührmann R. 1996. In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes. Nucleic Acids Res 24: 4614-4623. (Pubitemid 26413336)
    • (1996) Nucleic Acids Research , vol.24 , Issue.23 , pp. 4614-4623
    • Will, C.L.1    Rumpler, S.2    Gunnewiek, J.K.3    Van Venrooij, W.J.4    Luhrmann, R.5
  • 36
    • 0026500367 scopus 로고
    • Reconstituted mammalian U4/U6 snRNP complements splicing: A mutational analysis
    • Wolff T, Bindereif A. 1992. Reconstituted mammalian U4/U6 snRNP complements splicing: a mutational analysis. EMBO J 11: 345-359.
    • (1992) EMBO J , vol.11 , pp. 345-359
    • Wolff, T.1    Bindereif, A.2
  • 37
    • 0025177036 scopus 로고
    • The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5′ portion of the U4 small nuclear RNA
    • Xu Y, Petersen-Bjørn S, Friesen JD. 1990. The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5′ portion of the U4 small nuclear RNA. Mol Cell Biol 10: 1217-1225.
    • (1990) Mol Cell Biol , vol.10 , pp. 1217-1225
    • Xu, Y.1    Petersen-Bjørn, S.2    Friesen, J.D.3
  • 38
    • 0025941556 scopus 로고
    • U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction
    • Yean SL, Lin RJ. 1991. U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction. Mol Cell Biol 11: 5571-5577.
    • (1991) Mol Cell Biol , vol.11 , pp. 5571-5577
    • Yean, S.L.1    Lin, R.J.2


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