Protein analysis through Western blot of cells excised individually from human brain and muscle tissue

Analytical Biochemistry

Volumn 425, Issue 2, 2012, Pages 120-124

  Koob, A O ^a   Bruns, L ^a   Prassler, C ^a   Masliah, E ^b   Klopstock, T ^a   Bender, A ^a,c  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Therapiezentrum Burgau   (Germany)

Author keywords

Cytochrome oxidase; Laser capture microdissection; Single cell proteomics; Substantia nigra; Tyrosine hydroxylase

Indexed keywords

AMINO ACIDS; CYTOLOGY; ELECTRON TRANSPORT PROPERTIES; MICRODISSECTION; MUSCLE; PROTEINS; PROTEOMICS; TISSUE ENGINEERING;

CYTOCHROME OXIDASE; LASER CAPTURE MICRODISSECTION; SINGLE CELLS; SUBSTANTIA NIGRA; TYROSINE HYDROXYLASE;

CELLS;

CYTOCHROME C OXIDASE; MELANIN; TYROSINE 3 MONOOXYGENASE;

ARTICLE; ASTROCYTE; BRAIN CELL; CELL LEVEL; CELL SPECIFICITY; CONTROLLED STUDY; ELECTRON TRANSPORT; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; LASER CAPTURE MICRODISSECTION; MITOCHONDRIAL RESPIRATION; MUSCLE CELL; NERVE CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SINGLE CELL ANALYSIS; SUBSTANTIA NIGRA; WESTERN BLOTTING;

EID: 84859931352     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.02.034     Document Type: Article

References (29)

1. B.J. Xu Combining laser capture microdissection and proteomics: methodologies and clinical applications Proteomics Clin. Appl. 4 2010 116 123
2. M.R. Emmert-Buck, R.F. Bonner, P.D. Smith, R.F. Chuaqui, Z. Zhuang, S.R. Goldstein, R.A. Weiss, and L.A. Liotta Laser capture microdissection Science 274 1996 998 1001 (Pubitemid 26398425)
3. R.F. Bonner, M. Emmert-Buck, K. Cole, T. Pohida, R. Chuaqui, S. Goldstein, and L.A. Liotta Laser capture microdissection: molecular analysis of tissue Science 278 1997 1481 1483
4. N.L. Simone, R.F. Bonner, J.W. Gillespie, M.R. Emmert-Buck, and L.A. Liotta Laser-capture microdissection: opening the microscopic frontier to molecular analysis Trends Genet. 14 1998 272 276 (Pubitemid 28341788)
5. N.L. Simone, A.T. Remaley, L. Charboneau, E.F. Petricoin 3rd, J.W. Glickman, M.R. Emmert-Buck, T.A. Fleisher, and L.A. Liotta Sensitive immunoassay of tissue cell proteins procured by laser capture microdissection Am. J. Pathol. 156 2000 445 452 (Pubitemid 30643800)
6. N.L. Simone, C.P. Paweletz, L. Charboneau, E.F. Petricoin 3rd, and L.A. Liotta Laser capture microdissection: beyond functional genomics to proteomics Mol. Diagn. 5 2000 301 307 (Pubitemid 32622202)
7. N. Murugesan, J.A. Macdonald, Q. Lu, S.L. Wu, W.S. Hancock, and J.S. Pachter Analysis of mouse brain microvascular endothelium using laser capture microdissection coupled with proteomics Methods Mol. Biol. 686 2011 297 311
8. F. Fend, M.R. Emmert-Buck, R. Chuaqui, K. Cole, J. Lee, L.A. Liotta, and M. Raffeld Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis Am. J. Pathol. 154 1999 61 66 (Pubitemid 29031612)
9. L. Mouledous, S. Hunt, R. Harcourt, J.L. Harry, K.L. Williams, and H.B. Gutstein Proteomic analysis of immunostained, laser-capture microdissected brain samples Electrophoresis 24 2003 296 302 (Pubitemid 36175879)
10. R.B. Braakman, T.M. Luider, J.W. Martens, J.A. Foekens, and A. Umar Laser capture microdissection applications in breast cancer proteomics Methods Mol. Biol. 755 2011 143 154
11. H. He, G. Sun, and F. Ping Laser-capture microdissection and protein extraction for protein fingerprint of OSCC and OLK Artif. Cells Blood Substit. Immobil. Biotechnol. 37 2009 208 213
12. L. Mouledous, S. Hunt, R. Harcourt, J. Harry, K.L. Williams, and H.B. Gutstein Navigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samples Proteomics 3 2003 610 615 (Pubitemid 36570252)
13. D. Zhang, S.G. Lim, and E.S. Koay Proteomic identification of down-regulation of oncoprotein DJ-1 and proteasome activator subunit 1 in hepatitis B virus-infected well-differentiated hepatocellular carcinoma Int. J. Oncol. 31 2007 577 584
14. A.S. Rodriguez, B.H. Espina, V. Espina, and L.A. Liotta Automated laser capture microdissection for tissue proteomics Methods Mol. Biol. 441 2008 71 90
15. D. Zhang, and E.S. Koay Analysis of laser capture microdissected cells by 2-dimensional gel electrophoresis Methods Mol. Biol. 428 2008 77 91
16. D.K. Ornstein, J.W. Gillespie, C.P. Paweletz, P.H. Duray, J. Herring, C.D. Vocke, S.L. Topalian, D.G. Bostwick, W.M. Linehan, E.F. Petricoin 3rd, and M.R. Emmert-Buck Proteomic analysis of laser capture microdissected human prostate cancer and in vitro prostate cell lines Electrophoresis 21 2000 2235 2242 (Pubitemid 30435823)
17. W. Martinet, V. Abbeloos, N. Van Acker, G.R. De Meyer, A.G. Herman, and M.M. Kockx Western blot analysis of a limited number of cells: a valuable adjunct to proteome analysis of paraffin wax-embedded, alcohol-fixed tissue after laser capture microdissection J. Pathol. 202 2004 382 388 (Pubitemid 38325249)
18. W.G. Tatton, and C.E. Greenwood Rescue of dying neurons - a new action for deprenyl in Mptp parkinsonism J. Neurosci. Res. 30 1991 666 672
19. R.W. Taylor, M.J. Barron, G.M. Borthwick, A. Gospel, P.F. Chinnery, D.C. Samuels, G.A. Taylor, S.M. Plusa, S.J. Needham, L.C. Greaves, T.B.L. Kirkwood, and D.M. Turnbull Mitochondrial DNA mutations in human colonic crypt stem cells J. Clin. Invest. 112 2003 1351 1360 (Pubitemid 38057689)
20. S.L. Old, and M.A. Johnson Methods of microphotometric assay of succinate-dehydrogenase and cytochrome-c oxidase activities for use on human skeletal-muscle Histochem. J. 21 1989 545 555 (Pubitemid 19259801)
21. A. Bender, K.J. Krishnan, C.M. Morris, G.A. Taylor, A.K. Reeve, R.H. Perry, E. Jaros, J.S. Hersheson, J. Betts, T. Klopstock, R.W. Taylor, and D.M. Turnbull High levels of mitochondrial DNA deletions in substantia nigra neurons in aging and Parkinson disease Nat. Genet. 38 2006 515 517
22. A. Bender, R.M. Schwarzkopf, A. McMillan, K.J. Krishnan, G. Rieder, M. Neumann, M. Elstner, D.M. Turnbull, and T. Klopstock Dopaminergic midbrain neurons are the prime target for mitochondrial DNA deletions J. Neurol. 255 2008 1231 1235
23. G.M. Kunz Jr., and D.W. Chan The use of laser capture microscopy in proteomics research - a review Dis. Markers 20 2004 155 160 (Pubitemid 39314345)
24. S. Viswanathan, M. Unlu, and J.S. Minden Two-dimensional difference gel electrophoresis Nat. Protoc. 1 2006 1351 1358
25. T. Kondo Tissue proteomics for cancer biomarker development: laser microdissection and 2D-DIGE BMB Rep. 41 2008 626 634
26. S.M. Cowherd, V.A. Espina, E.F. Petricoin 3rd, and L.A. Liotta Proteomic analysis of human breast cancer tissue with laser-capture microdissection and reverse-phase protein microarrays Clin. Breast Cancer 5 2004 385 392 (Pubitemid 40109647)
27. O. Greengauz-Roberts, H. Stoppler, S. Nomura, H. Yamaguchi, J.R. Goldenring, R.H. Podolsky, J.R. Lee, and W.S. Dynan Saturation labeling with cysteine-reactive cyanine fluorescent dyes provides increased sensitivity for protein expression profiling of laser-microdissected clinical specimens Proteomics 5 2005 1746 1757 (Pubitemid 40686220)
28. H. Baker, V. Patel, A.A. Molinolo, E.J. Shillitoe, J.F. Ensley, G.H. Yoo, A. Meneses-Garcia, J.N. Myers, A.K. El-Naggar, J.S. Gutkind, and W.S. Hancock Proteome-wide analysis of head and neck squamous cell carcinomas using laser-capture microdissection and tandem mass spectrometry Oral Oncol. 41 2005 183 199 (Pubitemid 40202885)
29. D.E. Palmer-Toy, D.A. Sarracino, D. Sgroi, R. LeVangie, and P.E. Leopold Direct acquisition of matrix-assisted laser desorption/ionization time-of-flight mass spectra from laser capture microdissected tissues Clin. Chem. 46 2000 1513 1516