Optimizing saccharomyces cerevisiae induction regimes

Methods in Molecular Biology

Volumn 866, Issue , 2012, Pages 191-195

  Drew, David ^a   Kim, Hyun ^b  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b Seoul National University   (South Korea)

Author keywords

Membrane protein; Overproduction; Saccharomyces cerevisiae

Indexed keywords

MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

MEMBRANE PROTEINS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

SACCHAROMYCES CEREVISIAE;

EID: 84859859308     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-770-5_16     Document Type: Article

References (9)

1. Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK (2003) Global analysis of protein localization in budding yeast. Nature 425:686-691 (Pubitemid 37314307)
2. Campbell SG, Ashe MP (2007) An approach to studying the localization and dynamics of eukaryotic translation factors in live yeast cells. Methods Enzymol 431:33-45
3. Greene LE, Park YN, Masison DC, Eisenberg E (2009) Application of GFP-labeling to study prions in yeast. Protein Pept Lett 16:635-641
4. Guo Y, Au WC, Shakoury-Elizeh M, Protchenko O, Basrai M, Prinz WA, Philpott CC (2010) Phosphatidylserine is involved in the ferrichrome-induced plasma membrane traffi cking of Arn1 in Saccharomyces cerevisiae . J Biol Chem 285:39564-39573
5. Newstead S, Kim H, von Heijne G, Iwata S, Drew D (2007) High-throughput fl uorescentbased optimization of eukaryotic membrane protein overexpression and purifi cation in Saccharomyces cerevisiae . Proc Natl Acad Sci USA 104:13936-13941 (Pubitemid 350003316)
6. André N, Cherouati N, Prual C, Steffan T, Zeder-Lutz G, Magnin T, Pattus F, Michel H, Wagner R, Reinhart C (2006) Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci 15:1115-1126
7. Figler RA, Omote H, Nakamoto RK, Al-Shawi MK (2000) Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: high-yield expression and purifi cation of human P-glycoprotein. Arch Biochem Biophys 376:34-46 (Pubitemid 30205112)
8. Sonoda Y, Cameron A, Newstead S, Omote H, Moriyama Y, Kasahara M, Iwata S, Drew D (2010) Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. FEBS Lett 584:2539-2547
9. Kota J, Gilstring CF, Ljungdahl PO (2007) Membrane chaperone Shr3 assists in folding amino acid permeases preventing precocious ERAD. J Cell Biol 176:617-628 (Pubitemid 46333977)