Neurite outgrowth assessment using high content analysis methodology

Methods in Molecular Biology

Volumn 846, Issue , 2012, Pages 247-260

  Radio, Nicholas M ^a  

^a Thermo Fisher Scientific   (United States)

Author keywords

High content analysis; High content screening; Immunofluorescence; In vitro neurotoxicity; Neurite outgrowth; Neuronal differentiation; PC12 cells

Indexed keywords

ANIMAL; ARTICLE; CULTURE TECHNIQUE; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; METHODOLOGY; NEURITE; PC12 CELL; PHENOTYPE; PHYSIOLOGY; RAT; TISSUE MICROARRAY; ULTRASTRUCTURE;

ANIMALS; CELL CULTURE TECHNIQUES; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; NEURITES; PC12 CELLS; PHENOTYPE; RATS; TISSUE ARRAY ANALYSIS;

EID: 84859837157     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-536-7_22     Document Type: Article

References (15)

1. Radio N, Breier J, Shafer T, Mundy W (2008) Assessment of chemical effects on neurite outgrowth in PC12 cells using high content screening. Tox Sci 105, 106-118
2. Craig A, Banker G (1994) Neuronal polarity. Annu Rev Neurosci 17, 267-310 (Pubitemid 24117032)
3. Webb S, Monk C, Nelson C (2001) Mechanisms of postnatal neurobiological development: Implication for human development. Dev Neuropsychol 19, 147-171 (Pubitemid 32845926)
4. Ramakers G (2002) Rho proteins, mental retardation and the cellular basis of cognition. Trends Neurosci 25, 191-199 (Pubitemid 34615536)
5. Radio N, Mundy W (2008). Developmental neurotoxicity testing in vitro: models for assessing neurite outgrowth. Neurotoxicology 29, 361-376
6. Vaundry D, Stork P, Lazarovici P, Eiden L (2002) Signaling pathways for PC12 cell differentiation: Making the right connections. Science 296, 1648-1649 (Pubitemid 34579153)
7. Greene L (1977) A quantitative bioassay for nerve growth factor (NGF) activity employing a clonal pheochromocytoma cell line. Brain Res 133, 350-353 (Pubitemid 8171232)
8. Das K, Barone S (1999) Neuronal differentiation in PC12 cells is inhibited by chlorpyrifos and its metabolites: Is acetylcholinesterase inhibition the site of action? Toxicol Appl Pharmacol 160, 217-230 (Pubitemid 29536370)
9. Parran D, Mundy W, Barone S Jr (2001) Effects of methylmercury and mercuric chloride on differentiation and cell viability in PC12 cells. Toxicol Sci 59, 278-290 (Pubitemid 32149559)
10. Smith C, Eisenstein M (2005) Automated imaging: Data as far as the eye can see. Nat Methods 2, 547-555 (Pubitemid 41131161)
11. Dragunow M (2008) High-content analysis in neuroscience. Nat Rev Neurosci 9, 779-788
12. Giuliano K, Gough A, Taylor D, Vernetti L, Johnston P (2010) Early safety assessment using cellular systems biology yields insights into mechanisms of action. J Biomol Screen 15, 783-797
13. Neely M, Nicholls J (1995) Electrical activity, growth cone motility and the cytoskeleton. J Exp Biol 198, 1433-1446
14. Harrill J, Robinette B, Mundy W (2011) Use of high content image analysis to detect chemicalinduced changes in synaptogenesis in vitro. Toxicol In Vitro 25, 368-387
15. Heumann R, Valet G, Maison D, Kemper J, Reiser G, Hamprecht B (1977) Infl uence of the time in culture on cellular and neuronal properties of neuroblastoma x glioma hydrid cells. J Cell Sci 27, 141-155