Protocol for effective differentiation of 3T3-L1 cells to adipocytes

Analytical Biochemistry

Volumn 425, Issue 1, 2012, Pages 88-90

  Zebisch, Katja ^a   Voigt, Valerie ^a   Wabitsch, Martin ^b   Brandsch, Matthias ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b UNIVERSITY OF ULM   (Germany)

Author keywords

3T3 L1 cells; Adipocyte differentiation; Rosiglitazone

Indexed keywords

CELL CULTURE;

ADIPOCYTE DIFFERENTIATION; ADIPOCYTES; ADIPOGENESIS; AMERICAN TYPE CULTURE COLLECTIONS; CELL DIFFERENTIATION; ROSIGLITAZONE;

CELLS;

DEXAMETHASONE; DIMETHYL SULFOXIDE; INSULIN; ISOBUTYLMETHYLXANTHINE; ROSIGLITAZONE;

ADIPOCYTE; ADIPOGENESIS; ARTICLE; BIOCHEMISTRY; CELL CULTURE; CELL DIFFERENTIATION; CELL STRAIN 3T3; FIBROBLAST; HUMAN; PRIORITY JOURNAL;

EID: 84859739268     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.03.005     Document Type: Article

References (16)

1. H. Green, and O. Kehinde Sublines of mouse 3T3 cells that accumulate lipid Cell 1 1974 113 116
2. S.P. Poulos, M.V. Dodson, and G.J. Hausman Cell line models for differentiation: preadipocytes and adipocytes Exp. Biol. Med. 235 2010 1185 1193
3. H. Green, and O. Kehinde An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion Cell 5 1975 19 27
4. C.S. Rubin, A. Hirsch, C. Fung, and O.M. Rosen Development of hormone receptors and hormonal responsiveness in vitro: insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells J. Biol. Chem. 253 1978 7570 7578
5. 2α and 1-methyl-3-isobutyl xanthine Proc. Natl. Acad. Sci. USA 73 1976 4516 4520
6. Health Protection Agency, Health Protection Agency culture collections, < http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp? refId=86052701&collection=ecacc-gc >.
7. A. Mehra, I. Macdonald, and T.S. Pillay Variability in 3T3-L1 adipocyte differentiation depending on cell culture dish Anal. Biochem. 362 2007 281 283
8. American Type Culture Collection, Additional information about this cell line: ATCC CL-173, < http://www.lgcstandards-atcc.org/Attachments/2269.pdf >.
9. M. Wabitsch, R.E. Brenner, I. Melzner, M. Braun, P. Möller, E. Heinze, K.M. Debatin, and H. Hauner Characterization of a human preadipocyte cell strain with high capacity for adipose differentiation Int. J. Obes. Relat. Metab. Disord. 25 2001 8 15
10. P. Fischer-Posovszky, F.S. Newell, M. Wabitsch, and H.E. Tornqvist Human SGBS cells: a unique tool for studies of human fat cell biology Obes. Facts 1 2008 184 189
11. P. Tontonoz, S. Singer, B.M. Forman, P. Sarraf, J.A. Fletcher, C.D.M. Fletcher, R.P. Brun, E. Mueller, S. Altiok, H. Oppenheim, R.M. Evans, and B.M. Spiegelman Terminal differentiation of human liposarcoma cells induced by ligands for peroxisome proliferator-activated receptor γ and the retinoid X receptor Proc. Natl. Acad. Sci. USA 94 1997 237 241
12. E.D. Rosen, and O.A. MacDougald Adipocyte differentiation from the inside out Nat. Rev. Mol. Cell Biol. 7 2006 885 896
13. N.D. Oakes, C.J. Kennedy, A.B. Jenkins, D.R. Laybutt, D.J. Chisholm, and E.W. Kraegen A new antidiabetic agent, BRL 49653, reduces lipid availability and improves insulin action and glucoregulation in the rat Diabetes 43 1994 1203 1210
14. K.W. Lee, Y.H. Ku, M. Kim, B.Y. Ahn, S.S. Chung, and K.S. Park Effects of sulfonylureas on peroxisome proliferator-activated receptor γ activity and on glucose uptake by thiazolidinediones Diabetes Metab. J. 35 2011 340 347
15. H.H. Hwang, P.G. Moon, J.E. Lee, J.G. Kim, W. Lee, S.H. Ryu, and M.C. Baek Identification of the target proteins of rosiglitazone in 3T3-L1 adipocytes through proteomic analysis of cytosolic and secreted proteins Mol. Cells 31 2011 239 246
16. J.L. Ramírez-Zacarías, F. Castro-Muñozledo, and W. Kuri-Harcuch Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil Red O Histochemistry 97 1992 493 497