LC-MS quantification of rosmarinic acid and ursolic acid in the Ocimum sanctum Linn. leaf extract (Holy Basil, Tulsi)

Journal of Liquid Chromatography and Related Technologies

Volumn 35, Issue 5, 2012, Pages 634-650

  Shanmuga Sundaram, R ^a   Ramanathan, M ^b   Rajesh, R ^c   Satheesh, B ^d   Saravanan, D ^d  

^a JSS COLLEGE OF PHARMACY   (India)

^b PSG COLLEGE OF PHARMACY   (India)

^c Bioequivalence Centre   (India)

^d NATIONAL COLLEGE   (India)

Author keywords

Holy basil; Indian traditional herb; LC MS; Ocimum sanctum; Rosmarinic acid; Tulsi; Ursolic acid

Indexed keywords

HOLY BASIL; INDIAN TRADITIONAL HERB; LC-MS; OCIMUM SANCTUM; ROSMARINIC ACID; TULSI; URSOLIC ACID;

AMMONIUM COMPOUNDS; ATMOSPHERIC PRESSURE; ETHANOL; FORMIC ACID;

ACETONITRILE;

ACETONITRILE; ALCOHOL; AMMONIUM ACETATE; FORMIC ACID; OCIMUM SANCTUM EXTRACT; ROSMARINIC ACID; URSOLIC ACID;

ACCURACY; ARTICLE; DRUG DETERMINATION; FLOW RATE; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; MASS SPECTROMETRY; NONHUMAN; PLANT LEAF; QUANTITATIVE ANALYSIS; ROOM TEMPERATURE; SENSITIVITY AND SPECIFICITY;

OCIMUM TENUIFLORUM;

EID: 84859721208     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826076.2011.606583     Document Type: Article

References (28)

1. Jha, N. K.; Pandey, I. K.; Jha, A. K. Ocimum sanctum: Tulsi. Phytopharm. 2005, 6, 3-18.
2. Uma Devi, P. Radioprotective, Anticarcinogenic and Antioxidant Properties of the Indian Holy Basil, Ocimum sanctum (Tulasi). Ind. J. Exp. Biol. 2001, 39, 185-190. (Pubitemid 32223010)
3. Varier, N. V. K. Ocimum tenuiflorum Linn. In Indian Medicinal Plants: A Compendium of 500 Species; Orient Longman Ltd.: Madras, India, 1997; Collect. Vol. 4, 168-196.
4. Singh, S.; Majumdar, D. K.; Rehan, H. M. Evaluation of Anti-Inflammatory Potential of Fixed Oil of Ocimum sanctum (Holy Basil) and Its Possible Mechanism of Action. J. Ethnopharmacol. 1996, 54, 19-26. (Pubitemid 26379872)
5. Maulik, G.; Maulik, N.; Bhandari, V.; Kagan, V. E.; Pakrashi, S.; Das, D. K. Evaluation of Antioxidant Effectiveness of Few Herbal Plants. Free Radic. Res. 1997, 27, 221-228. (Pubitemid 27514052)
6. Mediratta, P. K.; Sharma, K. K.; Singh, S. Evaluation of Immunomodulatory Potential of Ocimum sanctum Seed Oil and Its Possible Mechanism of Action. J. Ethnopharmacol. 2002, 80, 15-20. (Pubitemid 34226635)
7. Sen, P.; Maiti, P. C.; Puri, S.; Ray, A.; Audulov, N. A.; Valdman, A. V. Mechanism of Antistress Activity of Ocimum sanctum Linn, Eugenol and Tinospora malabarica in Experimental Animals. Ind. J. Exp. Biol. 1992, 30, 592-596.
8. Devi, P. U.; Ganasoundari, A.; Vrinda, B.; Srinivasan, K. K.; Unnikrishnan, M. K. Radiation Protection by Ocimum Flavonoids Orientin and Vicenin: Mechanism of Action. Radiat. Res. 2000, 154, 455-460.
9. Yanpallewar, S. U.; Rai, S.; Kumar, M.; Acharya, S. B. Evaluation of Antioxidant and Neuroprotective Effect of Ocimum sanctum on Transient Cerebral Ischemia and Long-Term Cerebral Hypoperfusion. Pharmacol. Biochem. Behav. 2004, 79, 155-164. (Pubitemid 39237714)
10. Aruoma, O. I.; Halliwell, B.; Aeschbach, R.; Loliger, J. Antioxidant and Pro-Oxidant Properties of Active Rosemary Constituents: Carnosol and Carnosic Acid. Xenobiotica 1992, 22, 257-268.
11. Petersen, M.; Simmonds, M. S. J. Molecules of Interest. Rosmarinic Acid. Phytochem. 2003, 62, 121-125.
12. Venkateswara Rao, K. N.; Gopalakrishnan, V.; Kishore, B. V. S. H.; Rajendra Prasad, K.; Srikanth, P. Occurrence, Distribution and Biological Activity of Ursolic Acid: A Review. Ind. Drugs 2000, 38, 216-224.
13. Gupta, S. K.; Prakash, J.; Sushma, S. Validation of Traditional Claim of Tulsi, Ocimum sanctum Linn. As a Medicinal Plant. Ind. J. Exp. Biol. 2002, 40, 765-773.
14. Takeda, H.; Tsuji, M.; Inazu, M.; Egashira, T.; Matsumiya, T. Rosmarinic Acid and Caffeic Acid Produce Antidepressive-Like Effect in the Forced Swimming Test in Mice. Eur. J. Pharmacol. 2002, 449, 261-267.
15. Takeda, H.; Tsuji, M.; Miyamoto, J.; Matsumiya, T. Rosmarinic Acid and Caffeic Acid Reduce the Defensive Freezing Behavior of Mice Exposed to Conditioned Fear Stress. Psychopharmacol. 2002, 164, 233-235. (Pubitemid 36015589)
16. Pereira, P.; Tysca, D.; Oliveira, P.; Silva Brum, L. F.; Picada, J. N.; Ardenghi, P. Neurobehavioral and Genotoxic Aspects of Rosmarinic Acid. Pharmacol. Res. 2005, 52, 199-203. (Pubitemid 40981918)
17. Liu, J. Pharmacology of Oleanolic Acid and Ursolic Acid. J. Ethnopharmacol. 1995, 49, 57-68. (Pubitemid 26010740)
18. Liu, J. Oleanolic Acid and Ursolic Acid: Research Perspectives. J. Ethnopharmacol. 2005, 100, 92-94. (Pubitemid 41073528)
19. Janicsák, G.; Máthé, I. Densitometric Method for Parallel Measurement of Rosmarinic Acid and Caffeic Acid in Plant Samples. Acta Pharm. Hung 1998, 68, 259-262. (Pubitemid 28471902)
20. Verma, J. K.; Joshi, A. V. HPTLC Method for the Determination of Ursolic Acid from Ocimum sanctum Linn. (Tulsi) Leaves and Its Formulations. Ind. Drugs 2005, 42, 650-653.
21. Trute, A.; Nahrstedt, A. Separation of Rosmarinic Acid Enantiomers by Three Different Chromatographic Methods (HPLC, CE, GC) and Determination of Rosmarinic Acid in Hedera helix L. Phytochem. Anal. 1986, 7, 204-208.
22. Liu, A. H.; Li, L.; Xu, M.; Lin, Y. H.; Guo, H. Z.; Guo, D. A. Simultaneous Quantification of Six Major Phenolic Acids in the Roots of Salvia miltiorrhiza and Four Related Traditional Chinese Medicinal Preparations by HPLC-DAD Method. J. Pharm. Bio. Med. Anal. 2006, 41, 48-56.
23. Wang, H.; Provan, G. J.; Helliwell, K. Determination of Rosmarinic Acid and Caffeic Acid in Aromatic Herbs by HPLC. Food Chem. 2004, 87, 307-311. (Pubitemid 38581908)
24. Liao, Q.; Yang, W.; Jia, Y.; Chen, X.; Gao, Q.; Bi, K. LC-MS Determination and Pharmacokinetic Studies of Ursolic Acid in Rat Plasma After Administration of Traditional Chinese Medicinal Preparation Li-Ying Extract. Yakugaku Zasshi 2005, 125, 509-515. (Pubitemid 40874445)
25. Anandjiwala, S.; Kalola, J.; Rajani, M. Quantification of Eugenol, Luteolin, Ursolic Acid and Oleanolic Acid in Black (Krishna Tulasi) and Green (Sri Tulasi) Varieties of Ocimum sanctum Linn. Using HPTLC. J. AOAC Int. 2006, 89, 1467-1474. (Pubitemid 46009901)
26. Hakkim, F. L.; Shankar, C. G.; Girija, S. Chemical Composition and Antioxidant Property of Holy Basil (Ocimum sanctum Linn) Leaves, Stems and Inflorescence and Their In Vitro Callus Cultures. J. Agric. Food Chem. 2007, 55, 9109-9117. (Pubitemid 350105563)
27. International Conference on Harmonization. Regulatory Acceptance of Analytical Procedures and/or Acceptance Criteria (RAAPAC) Q4B; ICH: Geneva, 2006.
28. http://mathcentral.uregina.ca/QQ/database/QQ.09.01/carmen1.html (Accessed, July 10 2009).