Long-term effectiveness of autologous cultured limbal stem cell grafts in patients with limbal stem cell deficiency due to chemical burns

Clinical and Experimental Ophthalmology

Volumn 40, Issue 3, 2012, Pages 255-267

  Marchini, Giorgio ^a   Pedrotti, Emilio ^a   Pedrotti, Massimo ^b   Barbaro, Vanessa ^c   Di Iorio, Enzo ^c   Ferrari, Stefano ^c   Bertolin, Marina ^c   Ferrari, Barbara ^c   Passilongo, Mattia ^a   Fasolo, Adriano ^c   Ponzin, Diego ^c  

^a UNIVERSITY OF VERONA   (Italy)

^b SAN BORTOLO HOSPITAL   (Italy)

^c The Veneto Eye Bank Foundation   (Italy)

Author keywords

Cell therapy; Cornea; Limbal stem cell deficiency; Limbus; Penetrating keratoplasty

Indexed keywords

CORTICOSTEROID; CYTOKERATIN 12; CYTOKERATIN 19; CYTOKERATIN 3; FIBRIN GLUE; MUCIN 1; PROTEIN P63;

ADULT; AGED; ANALGESIA; ARTICLE; CHEMICAL BURN; CLINICAL ARTICLE; CLINICAL EFFECTIVENESS; CONJUNCTIVA; CORNEA DISEASE; CORNEA EPITHELIUM; CORNEA PERFORATION; CORNEA VASCULARIZATION; CYTOLOGY; EX VIVO STUDY; EYE PAIN; FEMALE; FOLLOW UP; GRAFT FAILURE; HISTOLOGY; HUMAN; INFLAMMATION; INTRAOCULAR PRESSURE ABNORMALITY; KERATECTOMY; KERATITIS; KERATOMYCOSIS; KERATOPLASTY; LIMBAL STEM CELL DEFICIENCY; LIMBAL STEM CELL TRANSPLANTATION; MALE; PHOTOPHOBIA; POSTOPERATIVE CARE; PROSPECTIVE STUDY; PROTEIN EXPRESSION; SURGICAL TECHNIQUE; TREATMENT OUTCOME; VISUAL ACUITY;

ADULT; AGED; BURNS, CHEMICAL; CELLS, CULTURED; CORNEAL DISEASES; EPITHELIUM, CORNEAL; EYE BURNS; FEMALE; FOLLOW-UP STUDIES; HUMANS; KERATOPLASTY, PENETRATING; LIMBUS CORNEAE; MALE; MIDDLE AGED; PROSPECTIVE STUDIES; STEM CELL TRANSPLANTATION; STEM CELLS; TRANSPLANTATION, AUTOLOGOUS; TREATMENT OUTCOME; VISUAL ACUITY; YOUNG ADULT;

EID: 84859697819     PISSN: 14426404     EISSN: 14429071     Source Type: Journal    
DOI: 10.1111/j.1442-9071.2011.02609.x     Document Type: Article

References (39)

1. Dua HS, Saini JS, Azuara-Blanco A, Gupta P. Limbal stem cell deficiency: concept, aetiology, clinical presentation, diagnosis and management. Indian J Ophthalmol 2000; 48: 83-92.
2. Kenyon KR, Tseng SC. Limbal autograft transplantation for ocular surface disorders. Ophthalmology 1989; 96: 709-22.
3. Chen JJ, Tseng SC. Corneal epithelial wound healing in partial limbal deficiency. Invest Ophthalmol Vis Sci 1990; 31: 1301-134.
4. Shi W, Gao H, Wang T, Xie L. Combined penetrating keratoplasty and keratolimbal allograft transplantation in comparison with corneoscleral transplantation in the treatment of severe eye burns. Clin Experiment Ophthalmol 2008; 36: 501-7.
5. Solomon A, Ellies P, Anderson DF etal. Long-term outcome of keratolimbal allograft with or without penetrating keratoplasty for total limbal stem cell deficiency. Ophthalmology 2002; 109: 1159-66.
6. Gallico GG III, O'Connor NE, Compton CC, Kehinde O, Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 1984; 311: 448-51.
7. Schermer A, Galvin S, Sun TT. Differentiation-related expression of a major corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 1986; 103: 49-62.
8. Ebato B, Friend J, Thoft RA. Comparison of central and peripheral human corneal epithelium in tissue culture. Invest Ophthalmol Vis Sci 1987; 28: 1450-6.
9. Dua HS, Azuara-Blanco A. Limbal stem cells of the corneal epithelium. Surv Ophthalmol 2000; 44: 415-25.
10. Pellegrini G, Traverso CE, Franzi AT, Zingirian M, Cancedda R, De Luca M. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 1997; 349: 990-3.
11. Pellegrini G, Golisano O, Paterna P etal. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J Cell Biol 1999; 145: 769-82.
12. Rama P, Bonini S, Lambiase A etal. Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation 2001; 72: 1478-85.
13. Shortt AJ, Secker GA, Notara MD etal. Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results. Surv Ophthalmol 2007; 52: 483-502.
14. Rama P, Matuska S, Paganoni G, Spinelli A, De Luca M, Pellegrini G. Limbal stem-cell therapy and long-term corneal regeneration. N Engl J Med 2010; 363: 147-55.
15. Nakamura T, Inatomi T, Sotozono C, Amemiya T, Kanamura N, Kinoshita S. Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders. Br J Ophthalmol 2004; 88: 1280-4.
16. Nishida K, Yamato M, Hayashida Y etal. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N Engl J Med 2004; 351: 1187-96.
17. Schwartz GS, Gomes JAP, Holland EJ. Preoperative staging of disease severity. In: Holland EJ, Mannis MJ, eds. Ocular Surface Disease: Medical and Surgical Management. New York, NY: Springer-Verlag, 2002; 158-67.
18. De Luca M, Pellegrini G, Green H. Regeneration of squamous epithelia from stem cells of cultured grafts. Regen Med 2006; 1: 45-57.
19. Harkin DG, Barnard Z, Gillies P, Ainscough SL, Apel AJ. Analysis of p63 and cytokeratin expression in a cultivated limbal autograft used in the treatment of limbal stem cell deficiency. Br J Ophthalmol 2004; 88: 1154-8.
20. Decreto 5 Dicembre. Gazzetta ufficiale della repubblica italiana. Serie Generale - n 2006; 57: 10-11.
21. Donisi PM, Rama P, Fasolo A, Ponzin D. Analysis of limbal stem cell deficiency by corneal impression cytology. Cornea 2003; 22: 533-8.
22. Dellambra E, Golisano O, Bondanza S etal. Downregulation of 14-3-3sigma prevents clonal evolution and leads to immortalization of primary human keratinocytes. J Cell Biol 2000; 149: 1117-30.
23. Di Iorio E, Barbaro V, Ruzza A, Ponzin D, Pellegrini G, De Luca M. Isoforms of deltaNp63 and the migration of ocular limbal cells in human corneal regeneration. Proc Natl Acad Sci U S A 2005; 102: 9523-8.
24. Kanayama S, Nishida K, Yamato M etal. Analysis of angiogenesis induced by cultured corneal and oral mucosal epithelial cell sheets in vitro. Exp Eye Res 2007; 85: 772-81.
25. Kanayama S, Nishida K, Yamato M etal. Analysis of soluble vascular endothelial growth factor receptor-1 secreted from cultured corneal and oral mucosal epithelial cell sheets in vitro. Br J Ophthalmol 2009; 93: 263-7.
26. Sekiyama E, Nakamura T, Kawasaki S, Sogabe H, Kinoshita S Different expression of angiogenesis-related factors between human cultivated corneal and oral epithelial sheets. Exp Eye Res 2006; 83: 741-6.
27. Lescai F, Conti L, Bartolozzi M etal. Genotype of inflammatory cytokines in limbal stem cell graft in Italian patients. Biochem Biophys Res Commun 2005; 332: 95-100.
28. Balasubramanian S, Jasty S, Sitalakshmi G, Madhavan HN, Krishnakumar S. Influence of feeder layer on the expression of stem cell markers in cultured limbal corneal epithelial cells. Indian J Med Res 2008; 128: 616-22.
29. Ronfard V, Rives JM, Neveux Y, Carsin H, Barrandon Y. Long-term regeneration of human epidermis on third degree burns transplanted with autologous cultured epithelium grown on a fibrin matrix. Transplantation 2000; 70: 1588-98.
30. Di Iorio E, Ferrari S, Fasolo A, Böhm E, Ponzin D, Barbaro V. Techniques for culture and assessment of limbal stem cell grafts. The Ocular Surface 2010; 8: 146-53.
31. Martin MJ, Muotri A, Gage F, Varki A. Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat Med 2005; 11: 228-32.
32. Shortt AJ, Secker GA, Rajan MS etal. Ex vivo expansion and transplantation of limbal epithelial stem cells. Ophthalmology 2008; 115: 1989-97.
33. Kolli S, Ahmad S, Lako M, Figueiredo F. Successful clinical implementation of corneal epithelial stem cell therapy for treatment of unilateral limbal stem cell deficiency. Stem Cells 2010; 28: 597-610.
34. Barbaro V, Ferrari S, Fasolo A etal. Evaluation of ocular surface disorders: a new diagnostic tool based on impression cytology and confocal laser scanning microscopy. Br J Ophthalmol 2010; 94: 926-32.
35. Colabelli Gisoldi RA, Pocobelli A, Villani CM, Amato D, Pellegrini G. Evaluation of molecular markers in corneal regeneration by means of autologous cultures of limbal cells and keratoplasty. Cornea 2010; 29: 715-22.
36. Di Iorio E, Barbaro V, Ferrari S, Ortolani C, De Luca M, Pellegrini G. Q-FIHC: quantification of fluorescence immunohistochemistry to analyse p63 isoforms and cell cycle phases in human limbal stem cells. Microsc Res Tech 2006; 69: 983-91.
37. Pellegrini G, Dellambra E, Golisano O etal. P63 identifies keratinocyte stem cells. Proc Natl Acad Sci U S A 2001; 98: 3156-61.
38. Inatomi T, Spurr-Michaud S, Tisdale AS, Gipson IK. Human corneal and conjunctival epithelia express MUC1 mucin. Invest Ophthalmol Vis Sci 1995; 36: 1818-27.
39. Chen WY, Mui MM, Kao WW, Liu CY, Tseng SC. Conjunctival epithelial cells do not transdifferentiate in organotypic cultures: expression of K12 keratin is restricted to corneal epithelium. Curr Eye Res 1994; 13: 765-78.