Evaluation of ocular surface disorders: A new diagnostic tool based on impression cytology and confocal laser scanning microscopy

British Journal of Ophthalmology

Volumn 94, Issue 7, 2010, Pages 926-932

  Barbaro, Vanessa ^a   Ferrari, Stefano ^a   Fasolo, Adriano ^a   Pedrotti, Emilio ^b   Marchini, Giorgio ^b   Sbabo, Arianna ^b   Nettis, Nicola ^a   Ponzin, Diego ^a   Di Iorio, Enzo ^a  

^a Ospedale Civile SS Giovanni e Paolo   (Italy)

^b UNIVERSITY OF VERONA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; CYTOKERATIN 12; CYTOKERATIN 19; CYTOKERATIN 3; MUCIN 1;

ADULT; AGED; ARTICLE; CHILD; CLINICAL ARTICLE; CONFOCAL LASER MICROSCOPY; CONJUNCTIVA; CONTROLLED STUDY; CORNEA LIMBUS; CYTODIAGNOSIS; CYTOLOGY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; EYE DISEASE; FLUORESCENCE; GENE EXPRESSION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; PRESCHOOL CHILD; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY;

ADULT; AGED; BIOLOGICAL MARKERS; CHILD, PRESCHOOL; CONJUNCTIVA; CONJUNCTIVAL DISEASES; CORNEA; CORNEAL DISEASES; CYTODIAGNOSIS; DIAGNOSTIC TECHNIQUES, OPHTHALMOLOGICAL; EYE PROTEINS; HUMANS; KERATINS; LIMBUS CORNEAE; MICROSCOPY, CONFOCAL; MIDDLE AGED; MUCIN-1;

EID: 77954253355     PISSN: 00071161     EISSN: 14682079     Source Type: Journal    
DOI: 10.1136/bjo.2009.164152     Document Type: Article

References (25)

1. Holly FJ, Lemp MA. Tear physiology and dry eye. Surv Ophthalmol 1977;22:69-87.
2. Gipson IK. Anatomy of the conjunctiva, cornea, and limbus. In: Smolin G, Thoft RA, eds. The cornea. Boston: Little, Brown, 1994;3-24.
3. Akpek EK, Foster CS. Limbal stem cells transplantation. Int Ophthalmol Clin 1999;39:71-81.
4. Kruse FE, Chen JJY, Tsai RJ, et al. Conjunctival transdifferentiation is due to the incomplete rmoval of limbal basal epithelium. Invest Ophthalmol Visual Sci 1990;31:1903-13.
5. Huang AJW, Tseng SCG. Corneal epithelial wound healing in the absence of limbal epithelium. Invest Ophthalmol Visual Sci 1991;32:96-105.
6. Dua HS, Azuara-Blanco A. Allo-limbal transplantation in patients with limbal stem cell deficiency. Br J Ophthalmol 1999;83:414-19. (Pubitemid 29161181)
7. Haamann P, Jensem OM, Schimidt P. Limbal autograft transplantation. Acta Ophthalmol Scand 1998;76:117-18.
8. Schermer A, Galvin S, Sun TT. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 1986;103:49-62. (Pubitemid 16066991)
9. Dua HS. The conjuntival in corneal epithelial wound healing. Br J Ophthalmol 1998;82:1407-11.
10. Tseng SC. Concept and application of limbal stem cells. Eye 1989;3:141-57.
11. Tseng SCG, Sun TT. Stem cells: ocular surface maintenance. In: Corneal surgery: theory, technique, and brightbill FS tissue. St Louis, Missouri: Mosby-Year Book, Inc, 2000:9-18.
12. Kenyon KR, Tseng SCG. Limbal autograft transplanatation for ocular surface disorders. Ophthalmology 1989;96:709-23.
13. Pellegrini G, Traverso CE, Franzi AT, et al. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 1997;349:990-3.
14. Rama P, Bonini S, Lambiase A, et al. Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation 2001;72:1478-85.
15. Donisi PM, Rama P, Fasolo A, et al. Analysis of limbal stem cell deficiency by corneal impression cytology. Cornea 2003;22:533-8.
16. Yoshida S, Shimmura S, Kawakita T, et al. Cytokeratin 15 can be used to identify the limbal phenotype in normal and diseased ocular surface. Invest Ophthalmol Vis Sci 2006;47:4780-6.
17. Chen WY, Mui MM, Kao WW, et al. Conjunctival epithelial cells do not transdifferentiate in organotypic cultures: expression of K12 keratin is restricted to corneal epithelium. Curr Eye Res 1994;13:765-78.
18. Di Iorio E, Barbaro V, Ruzza A, et al. Isoforms of ΔNp63 and the migration of ocular limbal cells in human corneal regeneration. Proc Natl Acad Sci 2005;102:9523-8.
19. Di Iorio E, Barbaro V, Ferrari S, et al. Quantification of fluorescent immunohistochemistry to analyse p63 isoforms and cell cycle phases in human limbal stem cells. Microsc Res Tech 2006;69:983-91.
20. Engelmann K, Kinlough CL, Müller S, et al. Transmembrane and secreted MUC1 probes show trafficking-dependent changes in O-glycan core profiles. Glycobiology 2005;15:1111-24.
21. Imbert Y, Darling DS, Jumblatt MM, et al. MUC1 splice variants in human ocular surface tissues: possible differences between dry eye patients and normal controls. Exp Eye Res 2006;83:493-501. (Pubitemid 43902771)
22. Inatomi T, SpurrpMichaud SJ, Tisdale AS, et al. Human corneal and conjunctival epithelia express MUC1 mucin. Invest Ophthalmol Vis Sci 1995;36:1818-27.
23. Gipson IK, Argüeso P. Role of mucins in the function of the corneal and conjunctival epithelia. Int Rev Cytol 2003;231:1-49. (Pubitemid 37549254)
24. Egbert PR, Laube S, Maurice DM. A simple conjunctival biopsy. Am J Ophthalmol 1977;84:798-801.
25. Kardon R, Price RE, Julian J, et al. Bacterial conjunctivitis in Muc1 null mice. Invest Ophthalmol Vis Sci 1999;40:1328-35. (Pubitemid 29252322)