Characterization of isolates of Citrus tristeza virus by sequential analyses of enzyme immunoassays and capillary electrophoresis-single-strand conformation polymorphisms

Journal of Virological Methods

Volumn 181, Issue 2, 2012, Pages 139-147

  Licciardello, G ^a   Raspagliesi, D ^a   Bar Joseph, M ^b   Catara, A ^a  

^a Science and Technology Park of Sicily scpa   (Italy)

^b GimlaoTec   (Israel)

Author keywords

Capillary electrophoresis; Citrus; CTV; Immunoassay; Single strands conformation analysis; Typing

Indexed keywords

COMPLEMENTARY DNA; PROTEIN P18; PROTEIN P23; PROTEIN P27; VIRUS RNA;

ARTICLE; CAPILLARY ELECTROPHORESIS; CITRUS; CITRUS TRISTEZA VIRUS INFECTION; CITRUS TRISTEZAVIRUS; CONTROLLED STUDY; DNA SYNTHESIS; DOT HYBRIDIZATION; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; GENE TARGETING; GENETIC VARIABILITY; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT DISEASE; PLANT LEAF; PLANT VIRUS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; RNA EXTRACTION; RNA ISOLATION; RNA VIRUS; SENSITIVITY AND SPECIFICITY; SEQUENTIAL ANALYSIS; SINGLE STRAND CONFORMATION POLYMORPHISM; VIRUS DETECTION; VIRUS GENE; VIRUS INFECTION; VIRUS ISOLATION; VIRUS MORPHOLOGY; VIRUS TRANSMISSION;

CITRUS; CLOSTEROVIRUS; ELECTROPHORESIS, CAPILLARY; IMMUNOBLOTTING; IMMUNOENZYME TECHNIQUES; PLANT DISEASES; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RNA, VIRAL; SICILY; VIROLOGY;

CITRUS; CITRUS TRISTEZA VIRUS;

EID: 84859427534     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.01.015     Document Type: Article

References (50)

1. Albiach-Martí M.R., Mawassi M., Gowda S., Satyanarayana T., Hilf M.E., Shanker S., Almira E.C., Vives M.C., López C., Guerri J., Flores R., Moreno P., Garnsey S.M., Dawson W.O. Sequences of Citrus tristeza virus separated in time and space are essentially identical. J. Virol. 2000, 74:6856-6865.
2. Ayllón M.A., Rubio L., Moya A., Guerri J., Moreno P. The haplotype distribution of two genes of Citrus tristeza virus is altered after host change or aphid transmission. Virology 1999, 255:32-39.
3. Bar-Joseph M., Dawson W.O. Citrus tristeza virus. Encyclopedia of Plant and Fungal Viruses 2008, 520-525. Elsevier, Oxford, UK. B.W.J. Mahy, M.H.V. Regenmortel (Eds.).
4. Bar-Joseph M., Garnsey S.M., Gonsalves D., Moscovitz M., Purcifull D.E., Clark M.F., Loebenstein G. The use of enzyme-linked immunosorbent assay for detection of Citrus tristeza virus. Phytopathology 1979, 69:190-194.
5. Bar-Joseph M., Marcus R., Lee R.F. The continuous challenge of Citrus tristeza virus control. Annu. Rev. Phytopathol. 1989, 27:291-316.
6. Bederski K., Roistacher C.N., Müller G.W. Cross protection against the severe Citrus tristeza virus: stem pitting in Peru. Proc. 16th Conf. Int. Org. Citrus Virol. 2005, 117-126.
7. Bertolini E., Moreno A., Copote N., Olmos A., De Luis A., Vidal E., Pérez-Panadés J., Cambra M. Quantitative detection of Citrus tristeza virus in plant tissues and single aphid by real-time RT-PCR. J. Plant Pathol. 2008, 120:177-188.
8. Broadbent P., Bevington K.B., Coote B.G. Control of stem pitting of grapefruit in Australia by mild strain cross protection. Proc. 11th Conf. Int. Org. Citrus Virol. 1991, 64-70.
9. Cambra M., Gorris M.T., Román M.P., Terrada E., Garnsey S.M., Camarasa E., Olmos A., Colomer M. Routine detection of Citrus tristeza virus by direct immunoprinting - ELISA method using specific monoclonal and recombinant antibodies. Proc. 14th Conf. Int. Org. Citrus Virol. 2000, 34-41.
10. Catara A., Barbagallo S., Saponari M. Il caso " tristeza" degli agrumi. I Georgofili-Quaderni 2008, 6:123-138.
11. Catara A., Davino M. Il virus della tristezza degli agrumi in Sicilia. Frutticoltura 2006, 1:18-23.
12. Catara A., Lombardo A., Nobile G., Rizza S. Characterization of additional Citrus tristeza virus isolates in a highly infected citrus area of Sicily. Proc. 17th Conf. Int. Org. Citrus Virol. 2010, pp. 80-83.
13. Costa A.S., Müller G.W. Tristeza control by cross-protection: US-Brazil cooperative success. Plant Dis. 1980, 64:538-541.
14. Dallot S., Boeglin M., Labonne G. Spatial pattern and genetic structure of PPV-M in a delimited area of stone fruit orchards in southern France. Acta Hort. 2008, 781:235-242.
15. Dawson W.O. Molecular genetics of Citrus tristeza virus. Citrus tristeza virus Complex and Tristeza Diseases 2010, 53-72. APS Press, The American Phytopathological Society, St. Paul, MN. A.V. Karasev, M.E. Hilf (Eds.).
16. D'Urso F., Sambade A., Moya A., Guerri J., Moreno P. Variation of haplotype distributions of two genomic regions of Citrus tristeza virus populations from eastern Spain. Mol. Ecol. 2003, 12:517-526.
17. Febres V.J., Ashoulin L., Mawassi M., Frank A., Bar-Joseph M., Manjunath K.L., Lee R.F., Niblett C.L. The p27 protein is present at one end of Citrus tristeza particles. Phytopathology 1996, 86:1331-1335.
18. Folimonova S.Y., Robertson C.J., Shilts T., Folimonov A.S., Hilf M.E., Garnsey S.M., Dawson W.O. Infection with strains of Citrus tristeza virus does not exclude superinfection by other strains of the virus. J. Virol. 2010, 84:1314-1325.
19. Garnsey S.M., Civerolo E.L., Gumpf D.J., Paul C., Hilf M.E., Lee R.F., Brlansky R.H., Yokomi R.K., Hartung J.S. Biological characterization of an international collection of Citrus tristeza virus (CTV) isolates. Proc. 16th Conf. Int. Org. Citrus Virol. 2005, 75-93.
20. Gillings M., Broadbent P., Indsto J., Lee R. Characterization of isolates and strains of Citrus tristeza closterovirus using restriction analysis of the coat protein gene amplified by the polymerase chain reaction. J. Virol. Methods 1993, 44:305-317.
21. Grant T.J., Higgins R.P. Occurrence of mixtures of tristeza virus strains in citrus. Phytopathology 1957, 47:272-276.
22. Harju V.A., Skelton A., Clover G.R.G., Ratti C., Boonham N., Henry C.M., Mumford R.A. The use of real-time RT-PCR TaqMan (R) and post-ELISA virus release for the detection of beet necrotic yellow vein virus types containing RNA 5 and its comparison with conventional RT-PCR. J. Virol. Methods 2005, 123:73-80.
23. Harper S.J., Dawson T.E., Pearson M.N. Isolates of Citrus tristeza virus that overcome Poncirus trifoliata resistance comprise a novel strain. Arch. Virol. 2010, 155:471-480.
24. Hilf M.E., Mavrodieva V.A., Garnsey S.M. Genetic marker analysis of a global collection of isolates of Citrus tristeza virus: characterization and distribution of CTV genotypes and association with symptoms. Phytopathology 2005, 95:909-917.
25. Jespersgaard C., Larsen L.A., Baba S., Kukita Y., Tahira T., Christiansen M., Vuust J., Hayashi K., Andersen P.S. Optimization of capillary array electrophoresis single-strand conformation polymorphism analysis for routine molecular diagnostics. Electrophoresis 2006, 27:3816-3822.
26. Karasev A.V., Boyko V.P., Gowda S., Nikolaeva O.V., Hilf M.E., Koonin E.V., Niblett C.L., Cline K., Gumpf D.J., Lee R.F., Garnsey S.M., Dawson W.O. Complete sequence of the Citrus tristeza virus RNA genome. Virology 1995, 208:511-520.
27. Karasev A.V., Hilf M.E. Citrus tristeza virus Complex and Tristeza Diseases 2010, APS Press, The American Phytopathological Society, St. Paul, MN.
28. Kong P., Rubio L., Polek M., Falk B.W. Population structure and genetic diversity within California Citrus tristeza virus (CTV) field isolates. Virus Genes 2000, 21:139-145.
29. Larsen L.A., Jespersgaard C., Andersen P.S. Single-strand conformation polymorphism analysis using capillary electrophoresis for large-scale mutation detection. Nat. Protoc. 2007, 2:1458-1466.
30. Lu R., Folimonov A., Shintaku M., Li W.X., Falk B.W., Dawson W.O., Ding S.W. Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc. Natl. Acad. Sci. U.S.A. 2004, 101:15742-15747.
31. Mawassi M., Mietkiewska E., Gofman R., Yang G., Bar-Joseph M. Unusual sequence relationships between two isolates of Citrus tristeza virus. J. Gen. Virol. 1996, 77:2359-2364.
32. Moreno P., Ambrós S., Albiach-Martí M.R., Guerri J., Peña L. Citrus tristeza virus: a pathogen that changed the course of the citrus industry. Mol. Plant Pathol. 2008, 9:251-268.
33. OEPP/EPPO Diagnostic protocols for regulated pests/Protocoles de diagnostic pour les organismes réglementés - Citrus tristeza closterovirus. OEPP/EPPO Bull. 2004, 34:155-157.
34. Permar T.A., Garnsey S.M., Gumpf D.J., Lee R.F. A monoclonal antibody which discriminates strains of Citrus tristeza virus. Phytopathology 1990, 80:224-228.
35. Quinto J.D., Wang C.K. A high-throughput single-stranded conformation polymorphism assay for detection, subtyping and genotyping of influenza viruses. Proc. Int. Congr. Ser. 2004, 1263:653-657.
36. Raspagliesi, D., Licciardello, G., Lombardo, A., Rizza, S., Bar-Joseph, M., Catara, A., 2010. Rapid detection and characterization of Citrus tristeza virus isolates by sequential ELISA/CE-SSCP. In: Proc. 18th Conf. Int. Org. Citrus Virol. IOCV, Riverside, CA., in press.
37. Raspagliesi D., Licciardello G., Rizza S., Lombardo A., Catara A. Quick characterization of Citrus tristeza virus isolates by capillary electrophoresis-single-strand conformation polymorphism. Acta Hort. 2011, 892:183-188.
38. Rosner A., Bar-Joseph M. Diversity of Citrus tristeza virus strains indicated by hybridization with cloned cDNA sequences. Virology 1984, 139:189-193.
39. Roy A., Ananthakrishnan G., Hartung J.S., Brlansky R.H. Development and application of a multiplex reverse-transcription polymerase chain reaction assay for screening a global collection of Citrus tristeza virus isolates. Phytopathology 2010, 100:1077-1088.
40. Rubio L., Ayllón M.A., Kong P., Fernández A., Polek M.L., Guerri J., Moreno P., Falk B.W. Genetic variation of Citrus tristeza virus isolates from California and Spain: evidence for mixed infections and recombination. J. Virol. 2001, 75:8054-8062.
41. Ruiz-Ruiz S., Moreno P., Guerri J., Ambrós S. The complete nucleotide sequence of a severe stem pitting isolate of Citrus tristeza virus from Spain: comparison with isolates from different origins. Arch. Virol. 2006, 151:387-398.
42. Ruiz-Ruiz S., Moreno P., Guerri J., Ambrós S. Discrimination between mild and severe Citrus tristeza virus isolates with a rapid and highly specific real-time reverse transcription-polymerase chain reaction method using TaqMan LNA probes. Phytopathology 2009, 99:307-315.
43. Sambade A., Lòpez C., Rubio L., Flores R., Guerri J., Moreno P. Polymorphism of a specific region in gene p23 of Citrus tristeza virus allows discrimination between mild and severe isolates. Arch. Virol. 2003, 148:2325-2340.
44. Sambade A., Rubio L., Garnsey S.M., Costa N., Müller G.W., Peyrou M., Guerri J., Moreno P. Comparison of viral RNA populations of pathogenically distinct isolates of Citrus tristeza virus: application to monitoring cross-protection. Plant Pathol. 2002, 51:257-265.
45. Sambrook J., Russell D. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 3rd ed.
46. Sen B., Hamelin J., Bru-Adan V., Godon J.-J., Chandra T.S. Structural divergence of bacterial communities from functionally similar laboratory-scale vermicomposts assessed by PCR-CE-SSCP. J. Appl. Microbiol. 2008, 105:2123-2132.
47. Vives M.C., Rubio L., López C., Navas-Castillo J., Albiach-Martí M.R., Dawson W.O., Guerri J., Flores R., Moreno P. The complete genome sequence of the major component of a mild Citrus tristeza virus isolate. J. Gen. Virol. 1999, 80:811-816.
48. Weng Z., Barthelson R., Gowda S., Hilf M.E., Dawson W.O., Galbraith D.W., Xiong Z. Persistent infection and promiscuous recombination of multiple genotypes of an RNA virus within a single host generate extensive diversity. PLoS One 2007, 2:e917.
49. Yang N., Mathews D.M., Dodds J.A., Mirkov T.E. Molecular characterization of an isolate of Citrus tristeza virus that causes severe symptoms in sweet orange. Virus Genes 1999, 19:131-142.
50. Yokomi R.K., Saponari M., Sieburth P.J. Rapid differentiation and identification of potential severe strains of Citrus tristeza virus by real-time reverse transcription-polymerase chain reaction assays. Phytopathology 2010, 100:319-327.